Cationic liposome–DNA complexes as gene delivery vectors: Development and behaviour towards bone-like cells

Induction of genuine autophagy by cationic lipids in mammalian cells

“…3 Compared to viral vectors, nonviral delivery has the advantages of relatively high safety and ease of use. 4,5 A variety of nonviral vectors, including those based on calcium phosphate, 6 cationic lipids, 7,8 peptides 9 and polymers, 10 have been developed over the years. Among them, cationic lipids represent one of the most widely used classes of vectors, and many transfection products based on cationic lipids are commercially available.…”

Carrier mediated protein and peptide stabilization

“…They observed that the DNA encapsulated into both plain and chitosan-coated liposomes was protected against degradation by DNase I. In a similar study, Oliveira et al (2009) also prepared liposomes with 1, 2-dioleoyl-3-trimethylammonium propane (DOTAP)-2-dioleoyl-Sn-glycero-3-phosphatidylethanolamine (DOPE) and DOTAP-cholesterol; and observed DNA protection capacity. Their results showed that the resistance of cationic liposome/DNA complexes to DNase I was proportional to the increase in lipid/DNA charge ratio of the complexes (Figure 1).…”

The Mechanical Agitation Method of Gene Transfer for Ex-Vivo Gene Therapy

“…An exogenous gene in the lipoplexes would not have a chance to be released into the cytoplasm for gene expression if the endosomes are stable. Therefore, helper lipids are added to form lipoplexes to facilitate the endosomal escape of the exogenous gene (Herringson et al, 2009a(Herringson et al, , 2009bSavva et al, 2005 (Segura & Shea, 2001), cationic peptides consisting of poly-L-Lysine (D'Haeze et al, 2007;Mullen et al, 2000;Niidome et al, 1997), or other types of cationic proteins (De Lima et al, 1999;Jean et al, 2009;Lam et al, 2004;Lee et al, 2003;Oliveira et al, 2009;Vighi et al, 2007). These approaches produce DNA carrying complexes that are more stable.…”