Caveolins and flotillin-2 are present in the blood stages of Plasmodium vivax

Guzmán-Bracho, Carmen; Dunia, I.; Romano, Mirtha; Raposo, Graça; Rosa, Mercedes de la; Benedetti, E.L.; Pérez, H.

doi:10.1007/s00436-006-0139-6

Cited by 13 publications

(8 citation statements)

References 39 publications

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“…32 However, we consider it unlikely that host or parasitic caveolins are involved in the formation of these "caveolae," because we could not detect caveolein-1 in reticulocytes or in the P vivax IRBCs (supplemental Figure 2A-B). Although this contradicts the findings of Bracho et al, 57 we could not find genes encoding caveolins or flotillins in the P vivax genome, 58 nor records of caveolin in human red cells.…”

Section: Discussioncontrasting

confidence: 99%

Plasmodium vivax: restricted tropism and rapid remodeling of CD71-positive reticulocytes

Malleret

Li²,

Zhang

et al. 2015

Blood

172

209

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Key Points• Plasmodium vivax merozoites preferentially infect a subgroup of reticulocytes generally restricted to the bone marrow.• Accelerated "maturation" of infected reticulocytes.Plasmodium vivax merozoites only invade reticulocytes, a minor though heterogeneous population of red blood cell precursors that can be graded by levels of transferrin receptor (CD71) expression. The development of a protocol that allows sorting reticulocytes into defined developmental stages and a robust ex vivo P vivax invasion assay has made it possible for the first time to investigate the fine-scale invasion preference of P vivax merozoites. Surprisingly, it was the immature reticulocytes (CD71 1) that are generally restricted to the bone marrow that were preferentially invaded, whereas older reticulocytes (CD71 2 ), principally found in the peripheral blood, were rarely invaded. Invasion assays based on the CD71 1 reticulocyte fraction revealed substantial postinvasion modification. Thus, 3 to 6 hours after invasion, the initially biomechanically rigid CD71 1 reticulocytes convert into a highly deformable CD71 2 infected red blood cell devoid of host reticular matter, a process that normally spans 24 hours for uninfected reticulocytes. Concurrent with these changes, clathrin pits disappear by 3 hours postinvasion, replaced by distinctive caveolae nanostructures. These 2 hitherto unsuspected features of P vivax invasion, a narrow preference for immature reticulocytes and a rapid remodeling of the host cell, provide important insights pertinent to the pathobiology of the P vivax infection. (Blood. 2015;125(8):1314-1324

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Section: Discussioncontrasting

confidence: 99%

Plasmodium vivax: restricted tropism and rapid remodeling of CD71-positive reticulocytes

Malleret

Li²,

Zhang

et al. 2015

Blood

172

209

View full text Add to dashboard Cite

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“…This study is one of only a few since 1975 to investigate the structure and molecular make-up of Plasmodium CVCs (Aikawa et al , 1975; Barnwell et al , 1990; Barnwell, 1986; Aikawa et al , 1977; Atkinson et al , 1990; Bracho et al , 2006; Udagama et al , 1988; Matsumoto et al , 1988; Matsumoto et al , 1986) and the first to present these structures in 3-D. The ET imaging results presented here corroborate previous TEM reports showing numerous CVCs interspersed along the surface of the P. vivax and P. cynomolgi iRBCs with their caveolae open to the surface (Aikawa et al , 1975).…”

Section: Discussionmentioning

confidence: 99%

A 95 kDa protein of Plasmodium vivax and P. cynomolgi visualized by three‐dimensional tomography in the caveola–vesicle complexes (Schüffner's dots) of infected erythrocytes is a member of the PHIST family

Akinyi

Hanssen

Meyer

et al. 2012

Molecular Microbiology

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Summary Plasmodium vivax and P. cynomolgi produce numerous caveolae-vesicle complex (CVC) structures within the surface of the infected erythrocyte membrane. These contrast with the electron-dense knob protrusions expressed at the surface of P. falciparum infected erythrocytes. Here we investigate the 3-dimensional structure of the CVCs and the identity of a predominantly expressed 95 kDa CVC protein. Liquid chromatography - tandem mass spectrometry analysis of immunoprecipitates by monoclonal antibodies from P. cynomolgi extracts identified this protein as a member of the Plasmodium helical interspersed sub-telomeric (PHIST) superfamily with a calculated mass of 81 kDa. We named the orthologous proteins PvPHIST/CVC-8195 and PcyPHIST/CVC-8195, analyzed their structural features, including a PEXEL motif, repeated sequences and a C-terminal PHIST domain, and show that PHIST/CVC-8195 is most highly expressed in trophozoites. We generated images of CVCs in 3-D using electron tomography, and used immuno-Electron Tomography (ET) to show PHIST/CVC-8195 localizes to the cytoplasmic side of the CVC tubular extensions. Targeted gene disruptions were attempted in vivo. The pcyphist/cvc-8195 gene was not disrupted, but parasites containing episomes with the tgdhfr selection cassette were retrieved by selection with pyrimethamine. This suggests that PHIST/CVC-8195 is essential for survival of these malaria parasites.

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“…The tethers presumably provide a physical connection to the surrounding membranes, a suggestion that is consistent with the fact that the Maurer's clefts remain associated with the RBC membrane when an infected RBC is lysed. They do not appear to open onto the surface of the RBC as is the case for the caveoli observed in Plasmodium vivax‐ infected RBCs (Matsumoto et al ., 1988; Barnwell et al ., 1990; Bracho et al ., 2006).…”

Section: Discussionmentioning

confidence: 99%

Electron tomography of the Maurer's cleft organelles of Plasmodium falciparum‐infected erythrocytes reveals novel structural features

Hanssen

Sougrat

Frankland³

et al. 2007

Molecular Microbiology

121

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SummaryDuring intraerythrocytic development, the human malaria parasite, Plasmodium falciparum, establishes membrane-bound compartments, known as Maurer's clefts, outside the confines of its own plasma membrane. The Maurer's compartments are thought to be a crucial component of the machinery for protein sorting and trafficking; however, their ultrastructure is only partly defined. We have used electron tomography to image Maurer's clefts of 3D7 strain parasites. The compartments are revealed as flattened structures with a translucent lumen and a more electron-dense coat. They display a complex and convoluted morphology, and some regions are modified with surface nodules, each with a circular cross-section of~25 nm. Individual 25 nm vesicle-like structures are also seen in the erythrocyte cytoplasm and associated with the red blood cell membrane. The Maurer's clefts are connected to the red blood cell membrane by regions with extended stalk-like profiles. Immunogold labelling with specific antibodies confirms differential labelling of the Maurer's clefts and the parasitophorous vacuole and erythrocyte membranes. Spot fluorescence photobleaching was used to demonstrate the absence of a lipid continuum between the Maurer's clefts and parasite membranes and the host plasma membrane.

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Caveolins and flotillin-2 are present in the blood stages of Plasmodium vivax

Cited by 13 publications

References 39 publications

Plasmodium vivax: restricted tropism and rapid remodeling of CD71-positive reticulocytes

Plasmodium vivax: restricted tropism and rapid remodeling of CD71-positive reticulocytes

A 95 kDa protein of Plasmodium vivax and P. cynomolgi visualized by three‐dimensional tomography in the caveola–vesicle complexes (Schüffner's dots) of infected erythrocytes is a member of the PHIST family

Electron tomography of the Maurer's cleft organelles of Plasmodium falciparum‐infected erythrocytes reveals novel structural features

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