CD30 on stimulated CD4⁺ T lymphocytes in newborns regarding atopic heredity

Abstract

CD30 was initially described as Ki-1 Ag on Reed-Sternberg cells of Hodgkin's lymphoma and its and CD30L(+) expression on T cells in placenta were equally frequent in the atopic and non-atopic women. In this article we present a study of CD30 mean fluorescence intensity (MFI) on CB T CD4(+) cells. We tested the hypothesis that in newborns with atopy family history there is a changed CB T cells response after antigen stimulation comparing with those without atopy family history. The study population consisted of 31 newborn babies (29-breastfed, two non-breastfed) and their mothers. Eleven of them had positive and 20 had negative atopy family history. Performed tests included cord blood, which was a subject to flowcytometry analysis and was cultured for 24 h, cytokine production was measured (IFN- gamma, IL-4 and IL-12). Secondly, we measured total maternal and cord blood IgE levels. We studied CD30 MFI as in our studies in larger group of newborns, CD30 expression on CD4(+) T cells appeared to be very low. MFI of CD4(+) CD30(+) after PHA-stimulation (213.55: range: 41.77-434.51) was significantly increased compared to MFI of CD4(+) CD30(+) before PHA-stimulation (43.63: range 28.67-134.67)(p </= 0.001). Newborns with and without atopy family history were analyzed. We found no difference between MFI of CD4(+) CD30(+) on non-stimulated T cells in non-atopic (43.80: range 28.66-134.66) and atopic (43.30: range 29.12-80.92) (p > 0.05). After PHA stimulation MFI of CD4(+) CD30(+) in non-atopic (273.05 (range: 42.9-434.51) was significantly increased compared with the atopic newborns to MFI of 87.1 (range: 41.78-241.42) (p = 0.00). We have not found any correlation between MFI of CD4(+) CD30(+) and total maternal and total CB IgE levels. The role of CD30 in immunological response needs further research studies.

CD30 expression in relation to atopic heredity and allergic disease during childhood

“…In their article (1), Stencel‐Gabriel et al. repeatedly claim that their findings are similar to the results in one of our previously published articles (4), where we investigated surface expression of CD30 and CD30L in placenta in relation to maternal atopy.…”

“…In the December issue of Pediatric Allergy and Immunology , Stencel‐Gabriel et al. describe how surface levels of CD30 on cord blood‐derived CD4 + T cells correlate with atopic heredity and IgE levels in plasma of mothers and newborns (1). CD30 and its ligand (CD30L) were suggested to influence Th1/Th2 responses several years ago (2) and their role in allergic disorders has been implicated [reviewed in Bengtsson (3)].…”

Asthma and pregnancy: emerging evidence of epigenetic interactions in utero

“…A number of other novel markers have been identified in relation to allergic risk (maternal allergy) or subsequent allergic disease. This includes eotaxin, which was noted to be higher in cord blood of asthmatic women [44], and lower levels of induced CD30 expression on CD4 þ T cells in high-risk neonates [45]. Higher levels of other proallergic markers in cord blood have also been associated with increased development of allergic disease in the first 2 years of life, including Th2-associated macrophage-derived chemokine [MDC (CCL22)] levels [46].…”