2003
DOI: 10.1016/j.exphem.2003.08.010
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CD34+CDw90(Thy-1)+ subset colocated with mesenchymal progenitors in human normal bone marrow hematon units is enriched in colony-forming unit megakaryocytes and long-term culture-initiating cells

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Cited by 19 publications
(30 citation statements)
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References 43 publications
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“…It is generally accepted that BM-MSCs are a highly adhesive fibroblast-like cell type. Our previous results [16] jointly with data from others [17] indicate the existence of a population of non-adherent, small, round cells, with self-renewal and multilineage differentiation potential in adult bone marrow, and those cells are capable of forming CFU-Fs in vitro . Accordingly we have developed the “Pour-off” method to amplify the 1 st BM-MSC.…”
Section: Introductionsupporting
confidence: 70%
“…It is generally accepted that BM-MSCs are a highly adhesive fibroblast-like cell type. Our previous results [16] jointly with data from others [17] indicate the existence of a population of non-adherent, small, round cells, with self-renewal and multilineage differentiation potential in adult bone marrow, and those cells are capable of forming CFU-Fs in vitro . Accordingly we have developed the “Pour-off” method to amplify the 1 st BM-MSC.…”
Section: Introductionsupporting
confidence: 70%
“…38 In our study, cell surface marker expression of BRCs from the subject who underwent cell transplantation in the bone defect showed 65-fold and 5-fold increases in CD90þ and CD105þ, respectively (data not shown). This served as an indication that the BRC product was highly enriched with cells possessing mesenchymal and angiogenic potential and is in accordance with previous reports demonstrating enrichment of theses cell types with this cell-processing protocol.…”
Section: Discussionmentioning
confidence: 97%
“…[9][10][11][12][13][14] Several markers have been proposed for use in identification and positive selection of osteogenic cells from bone marrow, including STRO-1, 15 CD271, 16 CD49a, 17 CD146, 18 and CD105. 19 We have recently reported that the presence of hyaluronan (HA; also known as hyaluronic acid or hyaluronate) on the surface of freshly isolated marrow-derived cells can also be used as a marker for positive selection. 20 HA, is a common, but highly regulated component of extracellular matrix.…”
mentioning
confidence: 99%