1992
DOI: 10.1182/blood.v80.7.1693.bloodjournal8071693
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CD34+ marrow cells, devoid of T and B lymphocytes, reconstitute stable lymphopoiesis and myelopoiesis in lethally irradiated allogeneic baboons

Abstract: CD34+ cells devoid of detectable mature and immature T and B lymphocytes, expressing the CD2, CD10, and CD20 antigens, were isolated from marrows of three pairs of sex-mismatched, mixed lymphocyte culture (MLC) nonreactive, sibling baboons. Reciprocal transplants were performed between members of each pair, using the sex chromosomes, identified by standard cytogenetic techniques, as markers of the transplanted cells. Five animals from these three pairs were transplanted with 0.6 to 2.1 x 10(6)/kg of isolated c… Show more

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Cited by 14 publications
(12 citation statements)
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“…To investigate the expression pattern of SALL4 in normal hematopoiesis, we first sorted normal human BM cells into CD34+ and CD34− subgroups. Previous studies have demonstrated that repopulating human HSCs are contained in the CD34+ fraction, while the CD34− fraction includes more matured blood elements 25,26 . Later studies suggested that the CD34+ fraction could be further subdivided: the HSCs resided in the human CD34+/CD38– population, and the HPCs resided in the CD34+CD38+ population 27 .…”
Section: Resultsmentioning
confidence: 99%
“…To investigate the expression pattern of SALL4 in normal hematopoiesis, we first sorted normal human BM cells into CD34+ and CD34− subgroups. Previous studies have demonstrated that repopulating human HSCs are contained in the CD34+ fraction, while the CD34− fraction includes more matured blood elements 25,26 . Later studies suggested that the CD34+ fraction could be further subdivided: the HSCs resided in the human CD34+/CD38– population, and the HPCs resided in the CD34+CD38+ population 27 .…”
Section: Resultsmentioning
confidence: 99%
“…Although the expression of markers does not necessarily correlate with cell function, varying functional abnormalities of CD34 + cells in AA have been associated with cell phenotype. Scopes et al (1994) demonstrated a reduced frequency of CD34 + CD33 – cells: a subpopulation of cells thought to be part of the group capable of initiating sustained haemopoiesis (Andrews et al , 1992). Maciejewski et al (1994) determined that, in the slightly more mature population of colony forming cells, CD34 + CD33 + were also markedly decreased in AA.…”
Section: Discussionmentioning
confidence: 99%
“…Recurrence of disease could be related to contaminating tumour cells in the autograft or to drug resistance of tumour cells in vivo . Haemopoietic progenitor and stem cells are characterized by the expression of the CD34 antigen and are capable of reconstituting haemopoiesis of lethally irradiated baboons and of patients with malignant diseases following myeloablative high‐dose therapy (Berenson et al , 1991; Andrews et al ,1992; Brugger et al , 1994; Gorin et al , 1995; Schiller et al , 1995; Civin et al , 1996a, b; Bensinger et al , 1996; Hohaus et al , 1997; McQuaker et al , 1997). As a result, methods for large‐scale selection have been developed for the enrichment of CD34 + cells to reduce the proportion of tumour cells in bone marrow or blood‐derived autografts (Berenson et al , 1991; Moss et al , 1992; McNiece et al , 1997).…”
mentioning
confidence: 99%