CDC project on standardizing steroid hormone measurements

Vesper, Hubert W.; Botelho, Julianne Cook; Shacklady, Christopher; Smith, Akimi; Myers, Gary L.

doi:10.1016/j.steroids.2008.09.008

Cited by 57 publications

(26 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the 2/16 OHE ratio is reported to be reproducible during the day and during the menstrual cycle [93, 94], a more uniform and standardized criterion in sample collection would likely help reduce variability and increase precision of the measurement. Ongoing collaborative projects aimed at standardization and improvement of steroid hormone measurements should be extended to include estrogen metabolism studies [106]. …”

Section: Discussionmentioning

confidence: 99%

Urinary 2/16 estrogen metabolite ratio levels in healthy women: A review of the literature

Dallal

Taioli

2010

Mutation Research/Reviews in Mutation Research

View full text Add to dashboard Cite

This is a summary of the published literature on the urinary 2/16 estrogen metabolite ratio in human populations, and a report the observed range of normal values in healthy women. Original research studies that included the measurement of urinary estrogen metabolites in human subjects were identified through an extensive Medline search; 43 distinct studies were indentified, including a total of 6802 healthy women. The range of mean values of the 2/16 ratio measured with the ELISA method varied from 0.98 to 1.74; in studies of pre-menopausal women the range of mean values was 1.5 to 2.74, in studies of post-menopausal women mean values ranged from 1.15 to 2.25. The heterogeneity across studies was highly significant (p-value Q test: <0.0001). In multivariable analyses, only race confirmed its role as an independent predictor of 2/16 ratio (F value: 7.95; p value: 0.009), after adjustment for age and menopausal status. There appears to be a large body of data on the 2/16 urinary ratio in healthy women. However, summary estimates are difficult to perform due to the high variability of the published study-specific values. The data suggests that race may be a contributor to 2/16 urinary ratio levels.

show abstract

Section: Discussionmentioning

confidence: 99%

Urinary 2/16 estrogen metabolite ratio levels in healthy women: A review of the literature

Dallal

Taioli

2010

Mutation Research/Reviews in Mutation Research

View full text Add to dashboard Cite

show abstract

“…Indeed, we are unaware of any clinical investigations evaluating the potential correlates of each of estradiol, estrone, total testosterone, SHBG, proinflammatory cytokines, and CT-quantified regional adiposity in regulating basic lipid fractions (LDL-C, TG, TC, HDL-C and nonHDL-C) in women either across the full adult lifespan or in pre- vs postmenopausal individuals studied identically. Moreover, no investigations to date have employed the criterion-based methods, mass spectrometry, to quantify both sex hormones and SHBG, which is now recognized as crucial for validity, reliability, precision, specificity and sensitivity of sex-steroid measurements [7]. In prior studies by other study groups, specific adipocytokines associated with systemic inflammation (interleukins, TNFα) and/or insulin resistance (adiponectin) have in fact been linked to lipids in PCOS, pregnancy, African women, and women with rheumatoid arthritis [8–11].…”

Section: Introductionmentioning

confidence: 99%

Interleukins 6 and 8 and abdominal fat depots are distinct correlates of lipid moieties in healthy pre- and postmenopausal women

Veldhuis

Dyer

Trushin³

et al. 2016

Endocrine

View full text Add to dashboard Cite

Purpose Available data associate lipids concentrations in men with body mass index (BMI), anabolic steroids, age, and certain cytokines. Data are less clear in women, especially across the full adult lifespan, and when segmented by pre- and postmenopausal status. Methods Subjects: 120 healthy women (60 pre- and 60 postmenopausal) in Olmsted County, MN, USA, a stable well studied clinical population. Dependent variables: measurements of 10-hr fasting high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and triglycerides (TG). Independent variables: testosterone, estrone, estradiol, 5-alpha-dihydrotestosterone (DHT) and sex-hormone binding globulin (SHBG, by mass spectrometry); insulin, glucose, and albumin; abdominal visceral, subcutaneous and total abdominal fat [AVF, SCF, TAF by computerized tomography (CT) scan]; and a panel of cytokines [by enzyme-linked immunosorbent assay (ELISA)]. Multivariate forward-selection linear-regression analysis was applied constrained to P<0.01. Results Lifetime data: HDL-C was correlated jointly with age (P<0.0001, positively), AVF (P<0.0001, negatively), and IL-6 (0.0063, negatively), together explaining 28.1% of its variance (P=2.3 × 10−8). TC was associated positively with multivariate age only (P=6.9 × 10−4, 9.3% of variance). TG’s correlated weakly with SHBG (P=0.0115), and strongly with AVF (P<0.0001), and IL-6 (P=0.0016) all positively (P=1.6 × 10−12, 38.9% of variance). NonHDL-C and LDL-C correlated positively with both TAF and IL-8 (P=2.0 × 10−5, 16.9% of variance; and P=0.0031, 9.4% of variance, respectively). Pre- vs postmenopausal comparisons identified specific relationships that were stronger in pre-than postmenopausal individuals, and vice versa. Age was a stronger correlate of LDL-C; IL-6 of TG and HDL; and both SHBG and TAF of nonHDL-C in pre- than postmenopausal women. Conversely, SHBG, AVF, IL-8, adiponectin were stronger correlates of TG; AVF and testosterone of HDL-C; and age of both nonHDL and LDL in post- than premenopausal women. Conclusion Our data delineate correlations of TAF and IL-8 (both positively) with nonHDL-C and LDL-C in healthy women across the full age range of 21–79 yr along with even more specific associations in pre- and postmenopausal individuals. Whether some of these outcomes reflect causal relationships would require longitudinal and interventional or genetic studies.

show abstract

“…However, the high costs of mass spectrometry equipment may be a possible drawback for routine clinical use. Although LC-MS/MS assays show higher precision and better accuracy than immunoassays, there is still a difference in performance (sensitivity, specificity, and trueness) among LC-MS/MS methods and thus there is a need for standardization (12).…”

Section: Introductionmentioning

confidence: 99%

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Janse

Eijkemans

Goverde

et al. 2011

European Journal of Endocrinology

View full text Add to dashboard Cite

Objective: The measurement of serum testosterone in women is challenging due to lack of trueness, precision, and sensitivity of various available testosterone assays. Accurate assessment of testosterone in women is crucial especially in conditions associated with alleged over-or under-production of testosterone, such as in polycystic ovary syndrome (PCOS) or primary ovarian insufficiency (POI). The aim of this study was to measure and compare androgen concentrations in women with PCOS, POI, and female controls and to evaluate the performance of extraction RIA and liquid chromatographytandem mass spectrometry (LC-MS/MS) in these women. Design: Cross-sectional study. Methods: Carefully phenotyped women with POI (nZ208) or PCOS (nZ200) and 45 healthy, regularly cyclic female controls were included. Method comparison analyses were performed for total testosterone, androstenedione (AD), and DHEA, as measured by LC-MS/MS and extraction RIA. Results: All androgen levels were significantly elevated in women with PCOS compared with POI patients (P!0.05) and controls (P!0.05). Women with POI presented with similar androgen concentrations as controls, except for AD. Compared with measurements by extraction RIA, testosterone, DHEA, and AD concentrations measured by LC-MS/MS were systematically lower. However, using extraction RIA and LC-MS/MS, testosterone, DHEA, and AD measurements were shown to have good agreement as assessed by Bland-Altman analysis and intraclass correlation coefficient: 0.95 (95% confidence interval 0. 94-0.91), 0.83 (0.79-0.86), and 0.96 (0.95-0.97) respectively. Conclusions: LC-MS/MS, compared with a labor-intensive extraction RIA, shows good precision, sensitivity, and high accuracy for measuring female testosterone, DHEA, and AD concentrations under various clinical conditions. LC-MS/MS, therefore, represents a convenient and reliable assay for both clinical and research purposes, where androgen measurement in women is required. European Journal of Endocrinology 165 925-933

show abstract

CDC project on standardizing steroid hormone measurements

Cited by 57 publications

References 23 publications

Urinary 2/16 estrogen metabolite ratio levels in healthy women: A review of the literature

Urinary 2/16 estrogen metabolite ratio levels in healthy women: A review of the literature

Interleukins 6 and 8 and abdominal fat depots are distinct correlates of lipid moieties in healthy pre- and postmenopausal women

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Contact Info

Product

Resources

About