cDNA clones of the auxin-binding protein from corn coleoptiles (Zea mays L): isolation and characterization by immunological methods

Molecular cloning and expression of the hot pepper ERabp1 gene encoding auxin-binding protein

“…This protein is primarily localized in the endoplasmic reticulum (ER), and includes a Lys-Asp-Glu-Leu (KDEL) C-terminal sequence which of may function as a signal for retaining the protein in the lumen of the ER [8]. cDNA ERabpl corresponding to this auxin-binding protein have been isolated from Arabidopsis [10] and maize [4,5,13,15].…”

“…It was reported that the maize ABP1 was . sequences were aligned with amino acid sequences derived from tobacco (EMBL accession number X70903), strawberry (EMBL accession number X91839), Arabidopsis [10] and maize [13]. Asterisks indicate identical amino acid residues in the five ERabpl.…”

RFLP mapping of the abp1 locus in maize (Zea mays L.)

“…The isolation of an ABP cDNA clone (pBS.ABP) is described elsewhere [ 10]. All restriction enzymes and the nick translation kit were purchased from Bethesda Research Laboratory, Bethesda, MD.…”

“…On a Southern blot developed under low stringency conditions, no additional bands specific for the ABP can be detected (data not shown). Of the restriction enzymes used to digest the genomic DNA, only Eco RI cuts the ABP cDNA, yielding two fragments similar in size [10]. When genomic DNA is probed, we detect only one Eco RI restriction fragment which hybridizes to the ABP cDNA (Fig.…”

“…First, the analysis of the cDNA clones isolated by different groups [4,5,10] reveals that the ABP is a soluble protein and lacks transmembrane domains as one would expect for a receptor protein. Second, the publications concerning the ABP cDNA have used ABP to name the isolated clones [5,10], with the exception of Hesse et al [4] who used axr-1. Third, we wish to avoid confusion with auxin resistance genes (,4xr-1) described by Estelle and Sommerville [3].…”