cDNA cloning and expression analysis of insulin-related peptide gene and prohormone convertase gene in the Pacific oyster, &lt;i&gt;Crassostrea gigas&lt;/i&gt;

Hamano, Kaoru; Awaji, Masahiko

doi:10.2331/fishsci.68.sup1_769

Cited by 6 publications

(4 citation statements)

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“…We conducted further phylogenetic analyses on the insulin-like peptides identified previously in the Pacific oyster genome 22 – 24 : cgMIP123 , cgMIP4 , cgMILP7 and cgILP , incorporating sequences from across the Metazoa (Supplementary Data 1 and Fig. 1 ).…”

Section: Resultsmentioning

confidence: 99%

Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals

Marlétaz

Gavriouchkina

et al. 2021

Nat Commun

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Hox and ParaHox genes encode transcription factors with similar expression patterns in divergent animals. The Pdx (Xlox) homeobox gene, for example, is expressed in a sharp spatial domain in the endodermal cell layer of the gut in chordates, echinoderms, annelids and molluscs. The significance of comparable gene expression patterns is unclear because it is not known if downstream transcriptional targets are also conserved. Here, we report evidence indicating that a classic transcriptional target of Pdx1 in vertebrates, the insulin gene, is a likely direct target of Pdx in Pacific oyster adults. We show that one insulin-related gene, cgILP, is co-expressed with cgPdx in oyster digestive tissue. Transcriptomic comparison suggests that this tissue plays a similar role to the vertebrate pancreas. Using ATAC-seq and ChIP, we identify an upstream regulatory element of the cgILP gene which shows binding interaction with cgPdx protein in oyster hepatopancreas and demonstrate, using a cell culture assay, that the oyster Pdx can act as a transcriptional activator through this site, possibly in synergy with NeuroD. These data argue that a classic homeodomain-target gene interaction dates back to the origin of Bilateria.

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Section: Resultsmentioning

confidence: 99%

Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals

Marlétaz

Gavriouchkina

et al. 2021

Nat Commun

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“…For example, the insulin-related peptide was identified in Mytilus edulis [5], Crassostrea gigas [6] and Anodonta cygnea [7], and the insulin receptor-related receptor that showed an association with insulin-like effects on growth was found in C . gigas [8].…”

Section: Introductionmentioning

confidence: 99%

“…Although most of these studies were implemented in vertebrate species, the IGF system has also been increasingly studied in invertebrates, and its members have been characterized in bivalve species. For example, the insulin-related peptide was identified in Mytilus edulis [5] , Crassostrea gigas [6] and Anodonta cygnea [7] , and the insulin receptor-related receptor that showed an association with insulin-like effects on growth was found in C . gigas [8] .…”

Section: Introductionmentioning

confidence: 99%

A Scallop IGF Binding Protein Gene: Molecular Characterization and Association of Variants with Growth Traits

Feng

et al. 2014

PLoS ONE

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BackgroundScallops represent economically important aquaculture shellfish. The identification of genes and genetic variants related to scallop growth could benefit high-yielding scallop breeding. The insulin-like growth factor (IGF) system is essential for growth and development, with IGF binding proteins (IGFBPs) serving as the major regulators of IGF actions. Although an effect of IGF on growth was detected in bivalve, IGFBP has not been reported, and members of the IGF system have not been characterized in scallop.ResultsWe cloned and characterized an IGFBP (PyIGFBP) gene from the aquaculture bivalve species, Yesso scallop (Patinopecten yessoensis, Jay, 1857). Its full-length cDNA sequence was 1,445 bp, with an open reading frame of 378 bp, encoding 125 amino acids, and its genomic sequence was 10,193 bp, consisting of three exons and two introns. The amino acid sequence exhibited the characteristics of IGFBPs, including multiple cysteine residues and relatively conserved motifs in the N-terminal and C-terminal domains. Expression analysis indicated that PyIGFBP was expressed in all the tissues and developmental stages examined, with a significantly higher level in the mantle than in other tissues and a significantly higher level in gastrulae and trochophore larvae than in other stages. Furthermore, three single nucleotide polymorphisms (SNPs) were identified in this gene. SNP c.1054A>G was significantly associated with both shell and soft body traits in two populations, with the highest trait values in GG type scallops and lowest in AG type ones.ConclusionWe cloned and characterized an IGFBP gene in a bivalve, and this report also represents the first characterizing an IGF system gene in scallops. A SNP associated with scallop growth for both the shell and soft body was identified in this gene. In addition to providing a candidate marker for scallop breeding, our results also suggest the role of PyIGFBP in scallop growth.

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“…IMP was able to bind to the mRNA of IGF2 and enhance its stability, and cytoplasmic IMP proteins could control IGF2 biosynthesis during late mammalian development (Nielsen et al, 1999;Nielsen et al, 2002;Szarvas et al, 2012). In bivalves, the IGF ligands (IGF1 and IGF2), IGF receptors and IGF-binding proteins are known to affect growth (Kellner-Cousin et al, 1994;Hamano and Awaji, 2002;Cong et al, 2013;Feng et al, 2014). Here, we found a SNP in PyIMP that was significantly associated with scallop growth, indicating the possible growth-regulating function of this gene, which was further supported by the association of PyIMP expression and the genotypes at this locus.…”

Section: Urochordata Cephalochordatamentioning

confidence: 99%

The scallop IGF2 mRNA-binding protein gene <i>PyIMP</i> and association of a synonymous mutation with growth traits

et al. 2018

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Insulin-like growth factor 2 mRNA-binding proteins (IMPs) function in localization, stability and translational control of their target RNAs. In this study, we identified an IMP gene (PyIMP) from Yesso scallop, Patinopecten yessoensis. The complete DNA sequence of PyIMP was 22,875 bp, consisting of seventeen exons and sixteen introns. The full-length cDNA sequence was 3,293 bp, with an open reading frame of 1,776 bp, encoding 592 amino acids. PyIMP exhibited characters typical of IMPs, namely two RNA recognition motifs and four hnRNP K homology domains. Real-time quantitative reverse transcription PCR analysis indicated that PyIMP was universally expressed, with higher expression levels in the gonad of adult scallops, and in gastrulae and trochophore larvae at developmental stages. A synonymous mutation SNP, c.852A>G, which showed significant associations with growth traits of Yesso scallop, was identified in this gene. Scallops with the AA genotype at this locus had significantly higher trait values than those with the GG genotype for shell length, shell height, body weight, soft tissue weight and striated muscle weight (P < 0.05). Meanwhile, the expression of PyIMP in AA type scallops was significantly higher than that in the GG type, implying a positive effect of PyIMP on scallop growth. PyIMP represents the first mRNA-binding protein gene characterized in mollusks, and SNP c.852A>G will be useful for a better understanding of the role of mRNA-binding proteins in bivalves and for scallop breeding.

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cDNA cloning and expression analysis of insulin-related peptide gene and prohormone convertase gene in the Pacific oyster, <i>Crassostrea gigas</i>

Cited by 6 publications

References 4 publications

Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals

Evidence from oyster suggests an ancient role for Pdx in regulating insulin gene expression in animals

A Scallop IGF Binding Protein Gene: Molecular Characterization and Association of Variants with Growth Traits

The scallop IGF2 mRNA-binding protein gene <i>PyIMP</i> and association of a synonymous mutation with growth traits

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