cDNA cloning, high-level expression, purification, and characterization of an avian Cu,Zn superoxide dismutase from Peking duck

Molecular cloning, identification and functional characterization of a novel intracellular Cu–Zn superoxide dismutase from the freshwater mussel Cristaria plicata

“…The high activity of rCp-icCuZnSOD was probably attributable to the addition of Cu/Zn ions during induction, since the activity of rCp-icCuZnSOD from the same culture without Cu/Zn ions was only 3166 U/mg (data not shown). Sufficient metal ions binding into the expressed SOD protein is beneficial for maintaining the activity [3], and similar observation of high SOD activity was reported in Peking duck by Liu et al [30]. The expression of the cloned gene in E. coli and the fact that the recombinant protein possessed SOD activity in vitro confirmed that the gene we cloned from C. plicata was Fig.…”

“…SODs derived from a wide range of organisms are reported to have higher stability to pH and temperature change, and protease hydrolysis than most other enzymes [24,25,30]. The recombinant human CuZnSOD (rhCuZnSOD) was stable from 25 to 70 C, at pH 5.0e10.5, and resistant to 10 mol/L urea and 8% SDS [32].…”

“…Finally we found that the inducing scheme of prolonged induction (8 h) at 20 C and the addition of 0.5 mM Cu 2þ and 0.1 mM Zn 2þ was optimal. Under this condition, the bulk of the recombinant protein was expressed in a soluble form, the same as other reconstituted SOD from Peking duck produced in E. coli [30].…”

Purification and partial characterization of recombinant Cu, Zn containing superoxide dismutase of Cordyceps militaris in E. coli

“…2(A), lane 4). This is consistent with the observation in Q-Sepharose Fast Flow column by Osatomia et al [23] and Liu et al [24]. An 88.4% of SOD activity was recovered in the flow-through solution with a 3.3-fold increase in specific activity after this step.…”

Superoxide Dismutase from the Silkworm,Bombyx mori: Sequence, Distribution, and Overexpression

“…39) For example, bovine SOD retains most of its original activity after incubation at 70 C for 60 min. 40) On the other hand, Xenopus laevis SOD loses about 40% of its original activity after incubation at 60 C for 60 min, because Cys-150 in the SOD is more sensitive to heat than mammalian SOD. 39,41) The bmSOD was less thermostable than mammalian SOD.…”