Superoxide Dismutase from the Silkworm,<i>Bombyx mori</i>: Sequence, Distribution, and Overexpression

Yamamoto, Kohji; Zhang, Pingbo; Banno, Yutaka; Fujii, Hiroshi; Miake, Fumio; Kashige, Nobuhiro; Aso, Yoichi

doi:10.1271/bbb.69.507

Cited by 31 publications

(35 citation statements)

References 44 publications

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“…This method gave positive SOD signals, which showed no difference in size from tissue to tissue; the existence of isozymes in the tissues was undetectable by the activity staining. Similar results have been obtained when the expression of bmSOD was examined (Yamamoto et al, 2005b). The fat body of the silkworm is a prime site for synthesis of antioxidant enzymes and detoxification enzymes (Yamamoto et al, 2005a, c;Krishnan and Kodrík, 2006), although Krishnan and Kodrík showed, in another lepidopteran insect, Spodoptera littoralis, that the SOD activity was specifically elevated in the midgut tissues of larvae fed a plant diet (Krishnan and Kodrík, 2006).…”

Section: Tissue Distribution Of the Transcript And Enzymesupporting

confidence: 61%

“…The resulting cDNA was used as a template to amplify a DNA fragment by PCR with the following two oligonucleotides primers: 5Ј-ATCTTGCTA-TACTCTAAATATAACA-3Ј (sense) and 5Ј-TATT-GTAGTACCAAGCCCAACATAT-3Ј (antisense). These were designed based on the 5Ј/3Ј UTR sequence of the SOD cDNA of the silkworm, bmSOD (Yamamoto et al, 2005b) (GenBank accession no. AB206971) and results of search for the SilkBase EST database (Bricker et al, 1990).…”

Section: Methodsmentioning

confidence: 99%

“…Spectra were measured as described in "Materials and Methods". Solid line and wavy line indicate the CD spectra of rhcSOD and rbmSOD, respectively; rbmSOD was obtained as described (Yamamoto et al, 2005b) and measured for CD spectra in parallel with rhcSOD. Fig.…”

Section: Characterization Of Hcsod and Comparison Of Its Properties Tmentioning

confidence: 99%

“…pH-stability and thermostability of rhcSOD and rbmSOD. Closed and open squares represent rhcSOD and rbmSOD, respectively; rbmSOD was obtained as described (Yamamoto et al, 2005b) and examined for stability in parallel with rhcSOD. (A) SODs were incubated at various pHs at 4°C for 24 h, and residual activities were determined.…”

Section: Characterization Of Hcsod and Comparison Of Its Properties Tmentioning

confidence: 99%

“…pressed the recombinant enzyme in Escherichia coli and compared its properties with those of the Bombyx mori homolog bmSOD (Yamamoto et al, 2005b). To the authors' knowledge, this report represents the first identification of cytosolic Cu/Zn-SOD protein of the fall webworm.…”

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confidence: 94%

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Characterization of superoxide dismutase from the fall webworm, Hyphantria cunea: Comparison of its properties to superoxide dismutase from the silkworm Bombyx mori

Yamamoto

Kimura

Aso

et al. 2007

Appl. Entomol. Zool.

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Section: Tissue Distribution Of the Transcript And Enzymesupporting

confidence: 61%

Section: Methodsmentioning

confidence: 99%

Section: Characterization Of Hcsod and Comparison Of Its Properties Tmentioning

confidence: 99%

Section: Characterization Of Hcsod and Comparison Of Its Properties Tmentioning

confidence: 99%

mentioning

confidence: 94%

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Characterization of superoxide dismutase from the fall webworm, Hyphantria cunea: Comparison of its properties to superoxide dismutase from the silkworm Bombyx mori

Yamamoto

Kimura

Aso

et al. 2007

Appl. Entomol. Zool.

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Microarray expression profiling of Spodoptera litura in response to oxidative stress

Yun¹,

Hwang²,

Yoon³

et al. 2011

Arch Insect Biochem Physiol

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To examine the expression profile of oxidative stress responsive genes in Spodoptera litura, we constructed a cDNA library from S. litura injected with hydrogen peroxide (H(2)O(2)). Using a microarray chip composed of 2,964 cDNAs, we screened gene expression at 1, 3, 5, 7, and 9 h post H(2)O(2) injection. Data were clustered into 15 groups of genes that behave similarly across each time course. Seventy-three genes were identified as being at least twofold up- or downregulated after treatment with H(2)O(2) in S. litura. We constructed expressed sequence tags (ESTs) for genes that changed at least twofold after treatment with H(2)O(2) . The functional classification of these ESTs based on Gene Ontology showed that the ESTs are rich in genes involved in oxidoreductase activity (5.7%), defense (14.3%), cellular process (22.9%), and development (17.1%).

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ANNOTATION AND EVOLUTION OF THE ANTIOXIDANT GENES IN THE SILKWORM, Bombyx mori

Guo-qing

Zhang

2012

Arch Insect Biochem Physiol

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Antioxidant system, which is composed of multiple gene families, plays a major role in reducing oxidative damage and xenobiotic detoxification in all living organisms. We identified 50 silkworm antioxidant genes from nine gene families based on the assembled genome sequence. A comparative analysis of the antioxidant genes of the silkworm with other order insects Anopheles gambiae, Apis mellifera, Drosophila melanogaster, and Tribolium castaneum, was performed. We found that most of the antioxidant gene families are highly conserved but Catalase (CAT) and heme-containing peroxidase (HPX) families were lineage-specifically expanded in the silkworm. The expression patterns of the silkworm antioxidant genes were investigated with the known ESTs, microarray data, and reverse transcription-polymerase chain reaction (RT-PCR). Forty two of the 50 silkworm antioxidant genes were transcribed and most of the transcribed genes showed tissue-specific expression patterns. More than a half of lineage-specifically expanded BmCATs lacked 15 or more than 15 of the 36 heme-binding residues and might lose catalase activities. However, the genes encoding these BmCATs showed almost a ubiquitous tissue expression pattern, indicating that they might have evolved new functions. In addition, the lineage-specifically expanded BmHPXs could function in maintaining cell homeostasis in the process of the synthesis of large amounts of silk proteins because they were predominantly expressed in silk gland of the silkworm. The lineage-specific expansion of antioxidant gene families in the silkworm provides useful information for understanding evolution and functional versatility of antioxidant genes in the silkworm even Lepidopteran insects.

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Superoxide Dismutase from the Silkworm,Bombyx mori: Sequence, Distribution, and Overexpression

Cited by 31 publications

References 44 publications

Characterization of superoxide dismutase from the fall webworm, Hyphantria cunea: Comparison of its properties to superoxide dismutase from the silkworm Bombyx mori

Characterization of superoxide dismutase from the fall webworm, Hyphantria cunea: Comparison of its properties to superoxide dismutase from the silkworm Bombyx mori

Microarray expression profiling of Spodoptera litura in response to oxidative stress

ANNOTATION AND EVOLUTION OF THE ANTIOXIDANT GENES IN THE SILKWORM, Bombyx mori

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