Cecal Ligation and Puncture as a Model of Sepsis in the Rat: Influence of the Puncture Size on Mortality, Bacteremia, Endotoxemia and Tumor Necrosis Factor Alpha Levels

Otero-Antón, Esteban; González‐Quintela, Arturo; López‐Soto, Alfonso; López‐Ben, Santiago; Llovo, J.; Pérez, L. Fernández-Cruz

doi:10.1159/000049698

Cited by 101 publications

(84 citation statements)

References 4 publications

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“…In present study, similar results were found as pus pocket formation and pus over caecum were more in rats who had larger caecal perforation. Caecal perforation size also seems to influence the systemic toxemia produced in peritonitis as proved by E. 8 The subjects were re-explored on the fifth day. This allowed enough time for the rats to come out of immediate postoperative stress and helped us to observe the short term results of the experimental drugs.…”

Section: Discussionmentioning

confidence: 99%

“…4,5 The organ dysfunction which is caused by peritonitis is worsened by free oxygen radicals those are the result of endotoxic shock. 6,7,8 The Caecal Ligation and Puncture (CLP) technique of peritonitis model in albino rats is good, economical and easily reproducible in experimental model for evaluation of new treatment modality. 9 The present study was carried out to assess the effect of intra-peritoneal Vitamin E and Metronidazole either singly or in combination, in experimentally produced faecal peritonitis model.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Study on Effect of Vitamin E as an Adjuvant to Metronidazole in Experimental Model of peritonitis

Shrivastava¹,

Gharde²,

Sharma

et al. 2013

Int J of Biomed & Adv Res

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Study on Effect of Vitamin E as an Adjuvant to Metronidazole in Experimental Model of peritonitis

Shrivastava¹,

Gharde²,

Sharma

et al. 2013

Int J of Biomed & Adv Res

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“…Sepsis was induced by the CLP model which was performed beforehand in different researches (13,14). After the administration of anesthesia under septic conditions, a 2 cm midline incision was made.…”

Section: Constitution Of Sepsis Modelmentioning

confidence: 99%

Neuromuscular Dysfunction in Experimental Sepsis and Glutamine

et al. 2016

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Background: Electrophysiological studies show that critical illness polyneuromyopathy appears in the early stage of sepsis before the manifestation of clinical findings. The metabolic response observed during sepsis causes glutamine to become a relative essential amino acid. Aims: We aimed to assess the changes in neuromuscular transmission in the early stage of sepsis after glutamine supplementation. Study Design: Animal experimentation. Methods: Twenty male Sprague-Dawley rats were randomized into two groups. Rats in both groups were given normal feeding for one week. In the study group, 1 g/kg/day glutamine was added to normal feeding by feeding tube for one week. Cecal ligation and perforation (CLP) surgery was performed at the end of one week. Before and 24 hours after CLP, compound muscle action potentials were recorded from the gastrocnemius muscle. Results: Latency measurements before and 24 hours after CLP were 0.68±0.05 ms and 0.80±0.09 ms in the control group and 0.69±0.07 ms and 0.73±0.07 ms in the study group (p<0.05). Conclusion: Since enteral glutamine prevented compound muscle action potentials (CMAP) latency prolongation in the early phase of sepsis, it was concluded that enteral glutamine replacement might be promising in the prevention of neuromuscular dysfunction in sepsis; however, further studies are required.

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“…Septik cevapta fizyopatolojik olaylar oldukça karmafl›k olmas›na ra¤men moleküler biyoloji alan›ndaki geliflmeler, sepsiste oluflan patolojik olaylar›n bir ço¤unun anlafl›lmas›n› mümkün k›lm›flt›r. Sepsiste ortaya ç›kan serbest oksijen radikalleri DNA'da hasara, hücresel proteinlerde denaturasyona ve membran lipitlerinde peroksidasyona yol açarak doku hasar›na neden olur (3,4) . Yap›lan çal›flmalarda sepsisde ortaya ç›kan serbest oksijen radikallerinin etkilerini nötralize etmek için uygulanan antioksidan ajanlar›n kullan›m›n sa¤ kal›m› uzatt›¤› gösterilmifltir (5) .…”

Section: G‹r‹fiunclassified

“…Çal›flmam›zda, deneysel sepsis modeli oluflturarak sentetik bir antioksidan ajan olan NAC ile antioksidan özelli¤ine ilaveten antibakteriyel özelli¤i bulunan Erdostein'i, doku hasar›na sebep olan veya fliddetini artt›ran mekanizmalara karfl› antioksidan tedavinin etkinlikleri aç›s›ndan karfl›laflt›rd›k. (9) . Laparotomi sonras› çekum izole edilerek, ç›kan kolon s›vazlanarak, çekum gaita ile doldurulduktan sonra ileoçekal valvin alt›ndan 3/0 ipek ile ba¤lan›p, çekum ön yüzü 18 numara intraket i¤nesi ile iki defa delindi.…”

Section: G‹r‹fiunclassified

Deneysel Sepsis Modelinde Glutatyon, Myeloperoksidaz, Plazma Ve Doku Mda Düzeylerine N-Asetilsistein Ve Erdostein’in Etkilerinin Karşılaştırılması

Ayan¹

2009

jaem

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SUMMARY Objectives:To determine the effects of NAC and erdostein as antioxidant agents on the free oxygen radicals and on their plasma levels. The role of NAC and erdostein on protecting the organ function failure and lung tissue damage due to sepsis was researched. Methods: In this study, 40 Sprague-Dawley rats were used. Rats were randomized to four equal groups. Sepsis was performed by the method of Caecum Ligation Perforation (CLP) on rats. Four groups were performed as sham group, sepsis group, sepsis+NAC (20mg/kg/day) and sepsis + erdostein (20mg/kg/day) NAC was given on 0. , 8. and 16.hours after CLP in NAC group. Erdostein was given on 0. and 12.hours after CLP. Blood samples were taken after 24 hours to determine the leukocyte, erythrocyte GSH, leukocyte MPO and plasma MDA levels. In addition, lung tissue samples were taken for histopathological studies and to determine the level of tissue MDA. Results: Comparing of groups on the level of leukocyte, leukocyte MPO and lung tissue MDA we determined statistically meaningful results in group 3 according to the group 2. But there were no meaningful differences on erythrocyte GSH and plasma MDA in group3 according to group 2(P>0.05). Comparing of groups on the level of erythrocyte GSH, leukocyte MPO plasma MDA and lung tissue MDA we determined statistically meaningful results in group 4 according to the group 2(P<0.05). But there were no meaningful differences on leukocyte in group 4 according to group 2(P>0.05). Conclusion:In this experimental sepsis model; it was determined that low dose NAC and erdostein as antioxidant agents have positive effects on erythrocyte GSH, leukocyte MPO, plasma and tissue MDA levels and the function of lung. Although the treatment with low dose NAC and erdostein has decreased the abnormality of organ function due to sepsis we couldn't show it histopathologically. We need more experimental studies to determine the effects of NAC and erdostein on sepsis therapy. ÖZET Amaç: Deneysel sepsis modelinde antioksidan bir ajan olan N-asetilsistein ve Erdostein'in serbest oksijen radikalleri üzerine olan etkileri ve sepsisin neden oldu¤u organ fonksiyon bozukluklar› ve akci¤er doku hasar›n› önlemedeki rolü araflt›r›ld›. Metod: Çal›flmada Sprague-Dawley cinsi 40 adet rat kullan›ld›. Ratlar randomize 10'arl› gruplara ayr›ld›lar. Ratlarda çekum ligasyon perforasyon yöntemiyle sepsis oluflturuldu. Sham grubu (grup 1), sepsis grubu (grup 2), sepsis + N-asetisistein (grup 3) ve sepsis + Erdostein (grup 4) fleklinde 4 grup oluflturuldu. N-asetilsistein grubunda ilaçlar (20mg/kg/gün), çekum perforasyonundan sonra, 0. 8. ve 16. saatlerde verildi. Erdostein grubunda ise ilaçlar(20mg/kg/gün) 0.ve 12. saatlerde verildi. 24. saatte lökosit, eritrosit glutatyon, lökosit myeloperoksidaz ve plazma malonildialdehit de¤erlerinin tayini için kan örnekleri ve doku incelemeleri için deneklerin ölümünü takiben akci¤erden doku örnekleri al›nd›. Bulgular: Gruplar karfl›laflt›r›ld›¤›nda grup 3'te grup 2'ye göre; lökosit, lökosit myeloperoksidaz ve akci¤er doku mal...

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Cecal Ligation and Puncture as a Model of Sepsis in the Rat: Influence of the Puncture Size on Mortality, Bacteremia, Endotoxemia and Tumor Necrosis Factor Alpha Levels

Cited by 101 publications

References 4 publications

A Study on Effect of Vitamin E as an Adjuvant to Metronidazole in Experimental Model of peritonitis

A Study on Effect of Vitamin E as an Adjuvant to Metronidazole in Experimental Model of peritonitis

Neuromuscular Dysfunction in Experimental Sepsis and Glutamine

Deneysel Sepsis Modelinde Glutatyon, Myeloperoksidaz, Plazma Ve Doku Mda Düzeylerine N-Asetilsistein Ve Erdostein’in Etkilerinin Karşılaştırılması

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