Cell adhesion and proliferation enhancement by gelatin nanofiber scaffolds

Wu, Shih-Ching; Chang, Wei-Hong; Dong, Guo-Chung; Chen, Kuo-Yu; Chen, Yueh-Sheng; Yao, Chun‐Hsu

doi:10.1177/0883911511423563

Cited by 157 publications

(89 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, biomaterial activity is still one key factor in designing an artificial bioscaffold (Kakisis et al 2005). To achieve that aim, numerous methods have been carried out (Ma et al 2005;Gouma et al 2010;Wu et al 2011;Truong et al 2012;Wang et al 2012). Among these methods, loading specific particulates was thought to be a facile one.…”

Section: Introductionmentioning

confidence: 99%

Effect of microcrystal cellulose and cellulose whisker on biocompatibility of cellulose-based electrospun scaffolds

Jia

Yang

et al. 2013

Cellulose

View full text Add to dashboard Cite

To investigate the potential application of microcrystal cellulose (MCC) and cellulose whisker (CW) in the electrospun vascular tissue scaffolds, cellulose acetate (CA) and cellulose composite scaffolds containing MCC and CW were electrospun from CA solutions and deacetylation. Structure and morphology of MCC, CW and the fibrous composite scaffolds were investigated using FT-IR, SEM, TEM and AFM. The wettability of the scaffolds was evaluated by water contact angle analysis. The effect of MCC and CW on the biocompatibility of the scaffolds for vascular smooth muscle cells (VSMC) was assayed by MTT test, fluorescent imaging and SEM. The biocomposite scaffolds displayed multiscaled structure and morphology. The scaffolds containing MCC and CW simultaneously exhibited significantly higher cell viability compared to those with only MCC or CW and no filler. Cell viability and morphology within the scaffolds become better with increasing content of MCC and CW. The composite scaffolds with both micro-and nano-scale organization could mimic the native extracellular matrix more closely, and further produce synergistic enhancement on VSMC viability, adhesion and proliferation. This study provides the potential applications of renewable cellulose-based particulates in biomedical field.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of microcrystal cellulose and cellulose whisker on biocompatibility of cellulose-based electrospun scaffolds

Jia

Yang

et al. 2013

Cellulose

View full text Add to dashboard Cite

show abstract

“…Biopolymer-based materials resembling components of the extracellular matrix (ECM), like collagen or gelatin, are promising candidates for soft tissue regeneration since they provide recognition motifs for cell adhesion and differentiation and are degradable under physiological conditions [1]. Current clinical applications of collagen or chemically modified collagen rest on its advantages of being tissue compatible and available from natural resources [2], but also display drawbacks as immunogenic responses [3], difficulty to tailor mechanical properties [4], and limitations in retaining dimensional stability.…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility and inflammatory response in vitro and in vivo to gelatin-based biomaterials with tailorable elastic properties

et al. 2014

View full text Add to dashboard Cite

Hydrogels prepared from gelatin and lysine diisocyanate ethyl ester provide tailorable elastic properties and degradation behavior. Their interaction with human aortic endothelial cells (HAEC) as well as human macrophages (Mɸ) and granulocytes (Gɸ) were explored. The experiments revealed a good biocompatibility, appropriate cell adhesion, and cell infiltration. Direct contact to hydrogels, but not contact to hydrolytic or enzymatic hydrogel degradation products, resulted in enhanced cyclooxygenase-2 (COX-2) expression in all cell types, indicating a weak inflammatory activation in vitro. Only Mɸ altered their cytokine secretion profile after direct hydrogel contact, indicating a comparably pronounced inflammatory activation. On the other hand, in HAEC the expression of tight junction proteins, as well as cytokine and matrix metalloproteinase secretion were not influenced by the hydrogels, suggesting a maintained endothelial cell function. This was in line with the finding that in HAEC increased thrombomodulin synthesis but no thrombomodulin membrane shedding occurred. First in vivo data obtained after subcutaneous implantation of the materials in immunocompetent mice revealed good integration of implants in the surrounding tissue, no progredient fibrous capsule formation, and no inflammatory tissue reaction in vivo. Overall, the study demonstrates the potential of gelatin-based hydrogels for temporal replacement and functional regeneration of damaged soft tissue.

show abstract

“…It has been reported that cross-linking of gelatin microspheres with GA takes place more rapidly and is more efficient than with genipin. A maximum crosslinking degree of approximately 90 % is achieved in less than 4 hours of exposure to GA, whereas just around 60 % of cross-linking was achieved after 72 h of exposure to genipin [21,22].…”

mentioning

confidence: 99%

Thermal and hydrolytic degradation of electrospun fish gelatin membranes

et al. 2013

View full text Add to dashboard Cite

The thermal and hydrolytic degradation of electrospun gelatin membranes cross-linked with glutaraldehyde in vapor phase has been studied. In vitro degradation of gelatin membranes was evaluated in phosphate buffer saline solution at 37 ºC. After 15 days under these conditions, a weight loss of 68 % was observed, attributed to solvation and depolymerization of the main polymeric chains. Thermal degradation kinetics of the gelatin raw material and as-spun electrospun membranes showed that the electrospinning processing conditions do not influence polymer degradation. However, for cross-linked samples a decrease in the activation energy was observed, associated with the effect of glutaraldehyde cross-linking reaction in the inter-and intra-molecular hydrogen bonds of the protein. It is also shown that the electrospinning process does not affect the formation of the helical structure of gelatin chains. Polymer Testing 32 (5), 995http://dx

show abstract

Cell adhesion and proliferation enhancement by gelatin nanofiber scaffolds

Cited by 157 publications

References 34 publications

Effect of microcrystal cellulose and cellulose whisker on biocompatibility of cellulose-based electrospun scaffolds

Effect of microcrystal cellulose and cellulose whisker on biocompatibility of cellulose-based electrospun scaffolds

Biocompatibility and inflammatory response in vitro and in vivo to gelatin-based biomaterials with tailorable elastic properties

Thermal and hydrolytic degradation of electrospun fish gelatin membranes

Contact Info

Product

Resources

About