Cell and Tissue Instructive Materials for Central Nervous System Repair

Rocha, L.A.; Silva, Deolinda; Barata‐Antunes, Sandra; Cavaleiro, Helena; Gomes, Eduardo D.; Silva, Nuno; Salgado, António J.

doi:10.1002/adfm.201909083

Cited by 23 publications

(13 citation statements)

References 252 publications

(430 reference statements)

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“…Addressing the above problems, one promising approach is to use scaffold materials to generate favorable microenvironments during stem cell implantation (13)(14)(15)(16). These scaffolds can help generate three-dimensional (3D) stem cell assembly, mitigate local inflammation, and establish favorable cell-extracellular matrix (ECM) interactions, typically mediated through focal adhesion kinase (FAK) signaling, which critically regulates neurogenesis and axon elongation (17)(18)(19)(20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

Hybrid SMART spheroids to enhance stem cell therapy for CNS injuries

et al. 2021

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Although stem cell therapy holds enormous potential for treating debilitating injuries and diseases in the central nervous system, low survival and inefficient differentiation have restricted its clinical applications. Recently, 3D cell culture methods, such as stem cell-based spheroids and organoids, have demonstrated advantages by incorporating tissue-mimetic 3D cell-cell interactions. However, a lack of drug and nutrient diffusion, insufficient cell-matrix interactions, and tedious fabrication procedures have compromised their therapeutic effects in vivo. To address these issues, we developed a biodegradable nanomaterial-templated 3D cell assembly method that enables the formation of hybrid stem cell spheroids with deep drug delivery capabilities and homogeneous incorporation of 3D cell-matrix interactions. Hence, high survival rates, controlled differentiation, and functional recovery were demonstrated in a spinal cord injury animal model. Overall, our hybrid stem cell spheroids represent a substantial development of material-facilitated 3D cell culture systems and can pave the way for stem cell-based treatment of CNS injuries.

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Section: Introductionmentioning

confidence: 99%

Hybrid SMART spheroids to enhance stem cell therapy for CNS injuries

et al. 2021

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“…The molecular chemical formula of aspirin is C9H8O4, the molecular structure formula is CH3COO-C6H4COOH, and the molecular relative mass is 180. 16.…”

Section: Methodsmentioning

confidence: 99%

“…Floium ginkgo extract and tertram ethypyrazine sodium chloride injection contain ginkgo biloba extract and ligustrazine phosphate, which is a compound preparation. This study observed the effect of floium ginkgo extract and tertram ethypyrazine sodium chloride injection in the treatment of cerebral infarction (13)(14)(15)(16)(17)(18)(19)(20)(21).…”

Section: Introductionmentioning

confidence: 99%

Effects of Floium ginkgo extract and tertram ethypyrazine sodium chloride injection on expression of inflammatory cytokines and cerebral infarction

Zhang¹,

Yuan²,

Liu³

et al. 2020

Cell Mol Biol (Noisy-le-grand)

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This experiment was carried out to observe and analyze the effect of floium ginkgo extract and tertram ethypyrazine sodium chloride injection in patients with cerebral infarction. A total of 200 patients diagnosed with cerebral infarction were enrolled in the study. They were randomly divided into a research group and control group, each containing 100 patients. The control group was given routine treatment measures while the research group was given floium ginkgo extract and tertram ethypyrazine sodium chloride injection on the basis of routine treatment. The therapeutic effects of the two groups were observed and compared. After implementing different treatment schemes, the levels of MMP-9, SOD, CBV and CBF in the research group were significantly higher than those in the control group, p<0.05. The research group was lower in hs-CRP, MDA, MTT, TTP and TNF-α as compared with the control group, p<0.05. In terms of the quality of life of the two groups after six months of treatment, the scores of various indicators in the research group were all significantly superior, p<0.05. Conclusion: The treatment of cerebral infarction patients with floium ginkgo extract and tertram ethypyrazine sodium chloride injection can significantly improve the therapeutic effect, which is a relatively ideal treatment.

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“…In order to uncover disease mechanisms and develop therapeutic strategies for brain damages and related neurological diseases, developing efficient neural regeneration models is significant and urgent. [ 1–5 ] As developed neural cells are no longer regenerative and proliferative cells, it is necessary to develop an induced cell line for neural regeneration. Induced pluripotent stem cells (IPS cells) are frequently used while obviating the necessity to obtain the tissue from the human brain or take the ethical issues destroying embryos.…”

Section: Introductionmentioning

confidence: 99%

Multivalent Polyanionic 2D Nanosheets Functionalized Nanofibrous Stem Cell‐based Neural Scaffolds

Xia

Yang

et al. 2021

Adv Funct Materials

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Because developed neural cells are no longer regenerative and proliferative, achieving neural regenerations by using induced pluripotent stem cells (IPS cells) for nerve diseases have recently attracted much attention. Since the IPS cells’ growth and differentiation can be manipulated by different physical and chemicals cues, scaffolds combining the beneficial nanostructures and extracellular matrix may become an ideal interface to promote IPS cells’ neural differentiation. In this work, a biocompatible and multivalent polyanion, hyperbranched polyglycerol sulfate, is used to modify the graphene oxide to obtain bio‐adhesive 2D nanosheets. After coating electrospinning nanofibers, the 2D nanosheets‐functionalized nanofibrous scaffolds are applied to mediate the proliferation, lineage specification, and neural differentiation of IPS cells. The results suggest that the modified scaffolds can improve the adhesion and proliferation of IPS cells combined with high efficiency in maintaining their stemness. During the neural differentiation process, the scaffolds can promote neural differentiation and their maturity, meanwhile decreasing the lineage specification toward astrocyte. Overall, this study not only provides new multivalent/bio‐adhesive nanofibrous scaffolds that integrate the chemical and physical cues to facilitate the targeted neural differentiation of IPS cells but also presents a novel pathway for the fabrication of carbon nanomaterials‐based biocomposites in regenerative therapies.

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Cell and Tissue Instructive Materials for Central Nervous System Repair

Cited by 23 publications

References 252 publications

Hybrid SMART spheroids to enhance stem cell therapy for CNS injuries

Hybrid SMART spheroids to enhance stem cell therapy for CNS injuries

Effects of Floium ginkgo extract and tertram ethypyrazine sodium chloride injection on expression of inflammatory cytokines and cerebral infarction

Multivalent Polyanionic 2D Nanosheets Functionalized Nanofibrous Stem Cell‐based Neural Scaffolds

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