Cell attachment and growth on films prepared from poly(depsipeptide‐co‐lactide) having various functional groups

Abstract

In tissue engineering related to the regeneration of damaged or lost tissue, excellent biodegradable materials are desired as temporary scaffolds to support cell growth and then disappear with the progress of tissue regeneration. We previously synthesized biodegradable poly(depsipeptide-co-lactide), poly[(Glc-Asp)-co-LA], and poly[(Glc-Lys)-co-LA] with reactive side-chain groups. In this study, to evaluate the utility of these copolymers as functional scaffolds for tissue regeneration, the effects of reactive and ionic side-chain groups on cell attachment and growth were investigated using copolymer films with various numbers of carboxyl or amino groups. Poly[(Glc-Lys)-co-LA] and poly[(Glc-Asp)-co-LA] films having appropriate positive or negative charges exhibited higher cell attachment ability than did poly-L-lactide. Good cell growth was observed on the copolymer films. During cell culture, the copolymer films exhibited higher degradation rates related to the depsipeptide content. Biodegradable polymer matrices with reactive surfaces for cell growth successfully were prepared using copolymers with various numbers of depsipeptide units. Varying the depsipeptide unit numbers in the copolymer could change the degradation rate of these matrices.

3-dimensional composite scaffolds consisting of apatite-PLGA-atelocollagen for bone tissue engineering

“…Cell attachment and proliferation are affected by the hydrophilic property, structure, and chemical composition of the scaffold surface. Also, the amount of cell adhesive proteins in aAC, such as fibronectin and vibronectin, subsequently dictate cell adhesion behavior 22,23) , because most cells attach to appropriately modified surfaces far better than to hydrophobic surfaces, including those with fewer proteins [24][25][26][27][28] . Moreover, collagen, specifically type I, enhances the adhesion and proliferation of cells, which is due to specific interactions with adhesion proteins 6,7) .…”

“…Our results demonstrated that PL-aAC had an excellent initial response toward soft tissues as compared with PLGA. The latter crumbled in the initial stage, because it did not have sufficient mechanical strength to resist the pressure 27) . In addition, PLGA degraded to acidic products and caused pH changes, which resulted in crumbling scaffolds and acidic products of PLGA lead to inflammation in vivo 8) .…”

Preparation of Plla Membranes With Different Morphologies for Culture of Ligament Cells

“…Protein modification of biomaterials has been reported to improve cell adhesion [5][6][7][8] and control the subsequent cellular response to material surfaces [9][10]. Current strategies in improving cell attachment and augmenting subsequent cellular response include pre-coating these surfaces with molecules such as laminin, fibronectin (Fn), collagen, and so on.…”

Synthesis of depsipeptides from L-amino acids and lactones

“…Many efforts had developed to introduce L-amino acids into polymer backbone for the depsipeptide [23], such as the synthesis of poly(depsipeptide-co-lactide)-g-poly(ethylene glycol) copolymers [24], poly(depsipeptides)-b-poly(ethylene glycol)-b-poly(depsipeptides) [25] and poly(depsipeptides) [26][27][28]. Ohya et al [29] had synthesized poly[(glutamic acid-aspartic)-colactic acid] with depsipeptide units. In cell culture, the copolymer films exhibited higher degradation rate which was related to the depsipeptide units and changed with varying depsipeptide content.…”