Cell-Based Biosensors in Clinical Chemistry

Kintzios, Spyridon

doi:10.2174/138955707782110141

Cited by 37 publications

(20 citation statements)

References 41 publications

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“…This lack of molecular selectivity has to be kept in mind if the solution an analytical problem, requires the specific detection of one Finally, one has to take into account, that even these high-profile cell-based sensor systems need relatively long assay times. Bioluminescence or other bioelectricity approaches demonstrated to be able to deliver a faster response toward toxic pollutants [45,46] compared to the above described sensor systems.…”

Section: Measurement Of Respiration Rates -Bionas 2500 Analyzing Systemmentioning

confidence: 99%

A critical comparison of cell-based sensor systems for the detection of Cr(VI) in aquatic environment

Bohrn

Mucha

Werner

et al. 2013

Sensors and Actuators B: Chemical

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a b s t r a c tThe toxicity of chromium ions was investigated using mammalian cell cultures on impedance sensors as well as physiological in vitro sensor systems. The performance of commercially available systems like the 2500 Analyzing System (Bionas), xCELLigence (Roche) and Cytosensor Microphysiometer (Molecular Devices) was compared with a novel CMOS-based impedance-to-frequency converter device. Cell-based sensor systems are shown to be powerful tools to detect Cr(VI) pollutions within several hours in the range of multinational drinking water regulations. The ability to distinguish between toxic Cr(VI) and non-toxic Cr(III) species is one advantage of these integral sensor systems. Impedance only devices are not sufficient for the fast detection of toxic chromium species as rapid cellular changes occur only in the respiration system and the cell physiology.

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Section: Measurement Of Respiration Rates -Bionas 2500 Analyzing Systemmentioning

confidence: 99%

A critical comparison of cell-based sensor systems for the detection of Cr(VI) in aquatic environment

Bohrn

Mucha

Werner

et al. 2013

Sensors and Actuators B: Chemical

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“…The biological elements embedded in the sensor interact with the test substance and generate measurable signals detectable by a recorder [2,3,4,5]. Different types of biological entities have been used on sensor platforms, including (but not limited to), antibodies, nucleic acids, enzymes, protein ligands, molecular imprints, receptors, living cells of both prokaryotic and eukaryotic origins.…”

Section: Introductionmentioning

confidence: 99%

“…These sensors may use bacteria, yeast or higher eukaryotic cells including vertebrate or mammalian cells. CBBs utilize the principles of cell-based assays (CBAs) and integrate that to an enclosed device platform [2,4,5,6] (Table 1). Therefore, CBBs can be construed as the “on-site” application enabler of a CBA for analyte testing.…”

Section: Introductionmentioning

confidence: 99%

Biotoxin Detection Using Cell-Based Sensors

Banerjee

Kintzios

Prabhakarpandian

2013

Toxins

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Cell-based biosensors (CBBs) utilize the principles of cell-based assays (CBAs) by employing living cells for detection of different analytes from environment, food, clinical, or other sources. For toxin detection, CBBs are emerging as unique alternatives to other analytical methods. The main advantage of using CBBs for probing biotoxins and toxic agents is that CBBs respond to the toxic exposures in the manner related to actual physiologic responses of the vulnerable subjects. The results obtained from CBBs are based on the toxin-cell interactions, and therefore, reveal functional information (such as mode of action, toxic potency, bioavailability, target tissue or organ, etc.) about the toxin. CBBs incorporate both prokaryotic (bacteria) and eukaryotic (yeast, invertebrate and vertebrate) cells. To create CBB devices, living cells are directly integrated onto the biosensor platform. The sensors report the cellular responses upon exposures to toxins and the resulting cellular signals are transduced by secondary transducers generating optical or electrical signals outputs followed by appropriate read-outs. Examples of the layout and operation of cellular biosensors for detection of selected biotoxins are summarized.

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“…In addition to established cell lines, primary cultures of cells obtained from human or other animal tissues have been used in recent years more broadly than before in the rapidly progressing field of cell engineering (Kintzios, 2007;Park and Lee, 2005;Sivaraman et al, 2005). As a result, the demand for a method by which to manipulate and analyze individual cells has been increasing.…”

Section: Introductionmentioning

confidence: 99%

Selective separation and co‐culture of cells by photo‐induced enhancement of cell adhesion (PIECA)

Edahiro¹,

Sumaru²,

Ooshima³

et al. 2008

Biotech & Bioengineering

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Taking advantage of the phenomenon that animal cells adhering to a culture substrate are temporarily immobilized by light irradiation, we established a technique to manipulate the cells adhering to a culture substrate under microscopic observation. Using this technique, we demonstrated a separation of cells adhering to a culture substrate and fabrication of an elaborately patterned co-culture system.

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Cell-Based Biosensors in Clinical Chemistry

Cited by 37 publications

References 41 publications

A critical comparison of cell-based sensor systems for the detection of Cr(VI) in aquatic environment

A critical comparison of cell-based sensor systems for the detection of Cr(VI) in aquatic environment

Biotoxin Detection Using Cell-Based Sensors

Selective separation and co‐culture of cells by photo‐induced enhancement of cell adhesion (PIECA)

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