Cell-based impedance spectroscopy for probing inhibitory effects of steroids and ergostane/lanosta-related compounds

Abstract

Electric cell-substrate impedance sensing (ECIS) was used for probing inhibitory effects on Spodoptera frugiperda Sf9 insect cells exposed to five synthesized steroid compounds. The results were compared to the levels obtained using three ergostane-related steroids and five lanosta-related triterpenes purified from the fruiting bodies of Antrodia camphorata. The half-inhibition concentration (ECIS 50 ), the level of 50% inhibition of the resistance response, was determined from the response function to establish inhibitory effects of the different compounds. Significant effects on inhibition as probed by impedance spectroscopy were noticed because of slight changes in chemical structure. Only two of the synthesized compounds, 24(R,S)-5a-lanost-8-ene-3,24,25-triol and 5a-lanost-8-ene-3,7,11-trione, showed inhibitory effects which were much less significant compared to the A. camphorata steroids. The ECIS 50 values were 150-250 mM, similar to the value for the least inhibitory lanosta-related triterpene. This noninvasive measurement in combination with Sf9 insect cells has been proven as a simple and reliable tool for screening inhibition/cytotoxicity and designing steroid-related compounds. Experimental MaterialsAll materials were obtained commercially (guaranteed reagent grade) and used without further purification.

Real-time characterization of cytotoxicity using single-cell impedance monitoring

“…10,11 Cell impedance measurements monitor electrical alternations between the cell and electrodes either at the interface where cells adhere to planar microelectrodes or dynamically in motion through microfluidic channels. 12 To date, cell impedance sensors have been employed to study the cytotoxic effects of a wide variety of substances, such as steroids, 13 air particulates, 14 drinking water contaminants and disinfectants, 15,16 nanoparticles, [17][18][19] and other organic and inorganic chemicals, [20][21][22][23] and have compared favorably to conventional cytotoxicity assays. 24,25 The conventional cell-impedance sensors generally study the behavior of multiple cells on largesized electrodes and provide data on the collective response of cells to a given physiological condition.…”

A novel and simple cell-based electrochemical impedance biosensor for evaluating the combined toxicity of DON and ZEN

“…Electrochemical impedance spectroscopy (EIS) is an alternative powerful tool for monitoring the changes in the interfacial properties at the electrode surfaces (Park and Yoo 2003). This technique has been used to detect interactions between biomolecules and cells (Wegener et al 1999), to develop many kinds of cell sensors (Chen et al 2005a;Yang et al 2004), and to analyze toxicity by detecting changes in resistance caused by cells attaching to and spreading on the electrode surface, cell morphology changes, and micromotion in the cell culture (Male et al 2010;Yoon et al 2014). …”

Electric cell–substrate impedance sensing technique to monitor cellular behaviours of cancer cells

“…ECIS is now an established, non-invasive electrochemical technique that has been successfully used to monitor the adhesion, growth and differentiation of cells in real time, [5][6][7][8][9][10] cell migration, [11][12][13][14] morphological changes during apoptosis, 15,16 single cell analysis by microuidic impedance cytometry, 17 and the toxic effects of drugs on cellular behaviour. [18][19][20][21][22] ZD6474 is a novel heteroaromatic-substituted anilinoquinazoline (Fig. S1 †), which inhibits two key pathways in tumour growth by targeting tumour growth indirectly, via inhibition of VEGF-dependent tumour angiogenesis and VEGF-dependent endothelial cell survival, [23][24][25][26] and also, by targeting tumour growth directly, via inhibition of EGFR-dependent tumour cell proliferation and survival.…”