Real-time characterization of cytotoxicity using single-cell impedance monitoring

Asphahani, Fareid; Thein, Maung Maung; Wang, Kui; Wood, David L.; Wong, Shawn; Xu, Jian; Zhang, Miqin

doi:10.1039/c2an16079j

Cited by 23 publications

(24 citation statements)

References 42 publications

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“…Several excitable mammalian cells (of neuronal or cardiac origin) used on micro-electrode array-integrated CBB platform provide electrical readouts to deduce toxicity results [57,58,59]. Many vertebrate and invertebrate cells used on CBBs for toxin detection measure electrical impedance [60,61], electrophysiology [62], or electric potentials [13,63] of cell and neighboring culture medium or matrices. It is important to note that the higher eukaryotic cell-based sensors can detect several biological toxins that microbial CBBs are unable to detect.…”

Section: Choice Of Biological Cells For Toxin Testingmentioning

confidence: 99%

Biotoxin Detection Using Cell-Based Sensors

Banerjee

Kintzios

Prabhakarpandian

2013

Toxins

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Cell-based biosensors (CBBs) utilize the principles of cell-based assays (CBAs) by employing living cells for detection of different analytes from environment, food, clinical, or other sources. For toxin detection, CBBs are emerging as unique alternatives to other analytical methods. The main advantage of using CBBs for probing biotoxins and toxic agents is that CBBs respond to the toxic exposures in the manner related to actual physiologic responses of the vulnerable subjects. The results obtained from CBBs are based on the toxin-cell interactions, and therefore, reveal functional information (such as mode of action, toxic potency, bioavailability, target tissue or organ, etc.) about the toxin. CBBs incorporate both prokaryotic (bacteria) and eukaryotic (yeast, invertebrate and vertebrate) cells. To create CBB devices, living cells are directly integrated onto the biosensor platform. The sensors report the cellular responses upon exposures to toxins and the resulting cellular signals are transduced by secondary transducers generating optical or electrical signals outputs followed by appropriate read-outs. Examples of the layout and operation of cellular biosensors for detection of selected biotoxins are summarized.

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Section: Choice Of Biological Cells For Toxin Testingmentioning

confidence: 99%

Biotoxin Detection Using Cell-Based Sensors

Banerjee

Kintzios

Prabhakarpandian

2013

Toxins

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“…To date, cell impedance measurements have been employed to study cytotoxic effects of a wide variety of substances [2][3][4][5] , cell response to drug treatment 3 , cell adhesion 6 , cell death 7 , cell cycle 8 , cell migration 9 and so on. To date, cell impedance measurements have been employed to study cytotoxic effects of a wide variety of substances [2][3][4][5] , cell response to drug treatment 3 , cell adhesion 6 , cell death 7 , cell cycle 8 , cell migration 9 and so on.…”

Section: Introductionmentioning

confidence: 99%

A microchip integrating cell array positioning with in situ single-cell impedance measurement

Guo

Zhu

Zong

2015

Analyst

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This paper presents a novel microarray chip integrating cell positioning with in situ, real-time and long-time impedance measurement on a single cell. The microchip integrates a plurality of quadrupole-electrode units (termed positioning electrodes) patterned into an array with pairs of planar electrodes (termed measuring electrodes) located at the centers of each quadrupole-electrode unit. The positioning electrodes are utilized to trap and position living cells onto the measuring electrodes based on negative dielectrophoresis (nDEP), while the measuring electrodes are used to measure impedances of the trapped single cells. Each measuring electrode has a small footprint area of 7 × 7 μm(2) to ensure inhabiting only one single cell on it. However, the electrode with a small surface area has a low double-layer capacitance when it is immersed in a liquid solution, thus generating a large double-layer impedance, which reduces the sensitivity for impedance measurement on the single cell. To enlarge the effective surface areas of the measuring electrodes, a novel surface-modification process is proposed to controllably construct gold nanostructures on the surfaces of the measuring electrodes while the positioning electrodes are unstained. The double layer capacitances of the modified electrodes are increased by about one order after surface-modification. The developed microchip is used to monitor the adhering behavior of a single HeLa cell by measuring its impedance spectra in real time. The measured impedance is analyzed and used to extract cellular electrical parameters, which demonstrated that the cell compresses the electrical double layer in the process of adherence and adheres onto the measuring electrodes after 4-5 hours.

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“…Advances in micro‐electro‐mechanical systems technologies have led to the development of many microfluidic devices in recent years, including micromixers , electrophoresis microchips , optical microflow cytometers , Coulter counters , micropumps , cell culture chips , and optofluidic microscopes . The growth and real‐time monitoring of cell cultures is essential in exploring the dynamic cellular processes involved in the expression of growth cells, cell migration, cell proliferation, and cell death.…”

Section: Introductionmentioning

confidence: 99%

Multichannel lens‐free CMOS sensors for real‐time monitoring of cell growth

Chang

Chen

et al. 2014

Electrophoresis

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A low-cost platform is proposed for the growth and real-time monitoring of biological cells. The main components of the platform include a PMMA cell culture microchip and a multichannel lens-free CMOS (complementary metal-oxide-semiconductor) / LED imaging system. The PMMA microchip comprises a three-layer structure and is fabricated using a low-cost CO2 laser ablation technique. The CMOS / LED monitoring system is controlled using a self-written LabVIEW program. The platform has overall dimensions of just 130 × 104 × 115 mm(3) and can therefore be placed within a commercial incubator. The feasibility of the proposed system is demonstrated using HepG2 cancer cell samples with concentrations of 5000, 10 000, 20 000, and 40 000 cells/mL. In addition, cell cytotoxicity tests are performed using 8, 16, and 32 mM cyclophosphamide. For all of the experiments, the cell growth is observed over a period of 48 h. The cell growth rate is found to vary in the range of 44∼52% under normal conditions and from 17.4∼34.5% under cyclophosphamide-treated conditions. In general, the results confirm the long-term cell growth and real-time monitoring ability of the proposed system. Moreover, the magnification provided by the lens-free CMOS / LED observation system is around 40× that provided by a traditional microscope. Consequently, the proposed system has significant potential for long-term cell proliferation and cytotoxicity evaluation investigations.

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Real-time characterization of cytotoxicity using single-cell impedance monitoring

Cited by 23 publications

References 42 publications

Biotoxin Detection Using Cell-Based Sensors

Biotoxin Detection Using Cell-Based Sensors

A microchip integrating cell array positioning with in situ single-cell impedance measurement

Multichannel lens‐free CMOS sensors for real‐time monitoring of cell growth

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