Cell behaviour in tubes

Riehle, Mathis; Ferris, Diana; Hamilton, David A.; Curtis, Adam

doi:10.1007/s00898-998-0002-6

Cited by 10 publications

(8 citation statements)

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“…This observation is further supported by the finding that bone precursor cells (chondrocytes, osteoblasts and epitenon cells) attach to concave surfaces inside tubes during culture (Ref. 90).…”

Section: Sintered Porous Hydroxyapatitesupporting

confidence: 72%

Tissue engineering: TGF-[beta] superfamily members and delivery systems in bone regeneration

Ramoshebi

Matsaba

Teare

et al. 2002

ERM

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The induction of bone formation requires three parameters that interact in a highly regulated process: soluble osteoinductive signals, capable responding cells, and a supporting matrix substratum or insoluble signal. The use of recombinant and naturally derived bone morphogenetic proteins and transforming growth factor βs (TGF-βs) has increased our understanding of the functions of these morphogens during the induction of endochondral bone formation. In addition, growing understanding of the cellular interactions of living tissues with synthetic biomaterials has led to the in vivo induction of bone formation using porous biomimetic matrices as an alternative to the use of autografts for bone regeneration. This review outlines the basis of bone tissue engineering by members of the TGF-β superfamily, focusing on their delivery systems and the intrinsic induction of bone formation by specific biomimetic matrices with a defined geometry.

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Section: Sintered Porous Hydroxyapatitesupporting

confidence: 72%

Tissue engineering: TGF-[beta] superfamily members and delivery systems in bone regeneration

Ramoshebi

Matsaba

Teare

et al. 2002

ERM

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“…As noted by Riehle et al [18], the hypothesis of Dunn and Heath [16], as well as that of O'Hara and Buck [17], do not explain cellular reaction to topographies such as holes or tubes. Our hypothesis based primarily on stable attachments and secondarily a cytoskeletal response to the attachment, would explain the reaction to such substrata.…”

Section: Gap Guidance Differs From Topographic Guidancementioning

confidence: 96%

“…Suggested mechanisms have generally focused on the relative inflexibility of cytoskeletal components as a primary regulator of cell elongation and alignment to surface features [16,17]. These theories fail to explain cellular reaction to holes or tubes [18], where more than actin filament and focal adhesion alignment may be involved. Both Brunette [19] and Walboomers et al [20] found that focal adhesions could bend round the edges of grooves.…”

Section: Introductionmentioning

confidence: 99%

“Gap guidance” of fibroblasts and epithelial cells by discontinuous edged surfaces

Hamilton

Brunette

2005

Experimental Cell Research

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“…4. Capillaries containing cells appeared consistently filled with cords [40] of cells possessing healthy nuclei at all time points (Fig. 4A, E, I); GFP positive cells appear attached and their processes spread along the capillaries at all time points as well (Fig.…”

Section: Resultsmentioning

confidence: 99%

Gelatinized Copper–Capillary Alginate Gel Functions as an Injectable Tissue Scaffolding System for Stem Cell Transplants

Willenberg

Zheng

Meng

et al. 2011

Journal of Biomaterials Science, Polymer Edition

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In severe hypoxic–ischemic brain injury, cellular components such as neurons and astrocytes are injured or destroyed along with the supporting extracellular matrix. This presents a challenge to the field of regenerative medicine since the lack of extracellular matrix and supporting structures makes the transplant milieu inhospitable to the transplanted cells. A potential solution to this problem is the use of a biomaterial to provide the extracellular components needed to keep cells localized in cystic brain regions, allowing the cells to form connections and repair lost brain tissue. Ideally, this biomaterial would be combined with stem cells, which have been proven to have therapeutic potentials, and could be delivered via an injection. To study this approach, we derived a hydrogel biomaterial tissue scaffold from oligomeric gelatin and copper–capillary alginate gel (GCCAG). We then demonstrated that our multipotent astrocytic stem cells (MASCs) could be maintained in GCCAG scaffolds for up to 2 weeks in vitro and that the cells retained their multipotency. We next performed a pilot transplant study in which GCCAG was mixed with MASCs and injected into the brain of a neonatal rat pup. After a week in vivo, our results showed that: the GCCAG biomaterial did not cause a significant reactive gliosis; viable cells were retained within the injected scaffolds; and some delivered cells migrated into the surrounding brain tissue. Therefore, GCCAG tissue scaffolds are a promising, novel injectable system for transplantation of stem cells to the brain.

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Cell behaviour in tubes

Cited by 10 publications

References 0 publications

Tissue engineering: TGF-[beta] superfamily members and delivery systems in bone regeneration

Tissue engineering: TGF-[beta] superfamily members and delivery systems in bone regeneration

“Gap guidance” of fibroblasts and epithelial cells by discontinuous edged surfaces

Gelatinized Copper–Capillary Alginate Gel Functions as an Injectable Tissue Scaffolding System for Stem Cell Transplants

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