Cell Block Preparation as a Diagnostic Technique Complementary to Fluid-Based Monolayer Cervicovaginal Specimens

Richard, Kathleen; Dziura, Bruce; Hornish, Annie

doi:10.1159/000330957

Cited by 43 publications

(54 citation statements)

References 8 publications

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“…Preparation (Richard et al, 1999) and detection of HPV DNA by PCR or in situ DNA hybridization (Maksem et al, 2001;Lee et al, 2006;Kavatkar et al, 2008;Deshou et al, 2009). …”

Section: Discussionmentioning

confidence: 99%

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Manual Liquid Based Cytology in Primary Screening for Cervical Cancer - a Cost Effective Preposition for Scarce Resource Settings

Nandini

Nandish²,

Pallavi³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Conventional pap smear (CPS) examination has been the mainstay for early detection of cervical cancer. However, its widespread use has not been possible due to the inherent limitations, like presence of obscuring blood and inflammation, reducing its sensitivity considerably. Automated methods in use in developed countries may not be affordable in the developing countries due to paucity of resources. On the other hand, manual liquid based cytology (MLBC) is a technique that is cost effective and improves detection of precursor lesions and specimen adequacy. Therefore the aim of the study was to compare the utility of MLBC with that of CPS in cervical cancer screening. A prospective study of 100 cases through MLBC and CPS was conducted from October 2009 to July 2010, in a Medical College in India, by two independent pathologists and correlated with histopathology (22 cases). Morphological features as seen through MLBC and CPS were compared. Subsequently, all the cases were grouped based on cytological diagnosis according to two methods into 10 groups and a subjective comparison was made. In order to compare the validity of MLBC with CPS in case of major diagnoses, sensitivity and specificity of the two methods were estimated considering histological examination as the gold standard. Increased detection rate with MLBC was 150%. The concordance rate by LBC/histopathology v/s CPS/histopathology was also improved (86% vs 77%) The percentage agreement by the two methods was 68%. MLBC was more sensitive in diagnosis of LSIL and more specific in the diagnosis of inflammation. Thus, MLBC was found to be better than CPS in diagnosis of precursor lesions. It provided better morphology with increased detection of abnormalities and preservation of specimen for cell block and ancillary studies like immunocytochemistry and HPV detection. Therefore, it can be used as alternative strategy for cervical cancer prevention in limited resource settings

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“…Preparation (Richard et al, 1999) and detection of HPV DNA by PCR or in situ DNA hybridization (Maksem et al, 2001;Lee et al, 2006;Kavatkar et al, 2008;Deshou et al, 2009). …”

Section: Discussionmentioning

confidence: 99%

“…Cell blocks from LBC specimens were found to aid in diagnosis of 20% of specimens in Thin prep preparation and were critical to the diagnosis in 5% of cases-cell blocks have the ability to reduce both the false positive and false negative rates of the lab test (Richard et al, 1999;Johnson et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Manual Liquid Based Cytology in Primary Screening for Cervical Cancer - a Cost Effective Preposition for Scarce Resource Settings

Nandini

Nandish²,

Pallavi³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…The results of the current study are in agreement with others that the addition of cell block to smears can be as valuable a diagnostic aid in gynecologic cytology as it is in nongynecologic cytology. 8,9,10 The current study was a retrospective study based primarily on known abnormal Pap smears with emphasis on HSIL and LSIL to determine the feasibility and validity of the cell block technique and its morphologic value in the diagnosis of intraepithelial lesions in addition to findings in ThinPrep smears. "Leftover" vials from completed cases, after any requests for additional ThinPrep slides or human papillomavirus DNA testing had been made, were retrieved before being discarded.…”

Section: Discussionmentioning

confidence: 99%

“…Smears with large clusters or true tissue fragments of abnormal cells are better visualized using the cell block technique. 8 Characterization of some lesions that may be difficult on smears is facilitated by histologic sectioning. Examples of difficult morphologic differential diagnoses include differentiating atypical immature squamous metaplasia from high-grade dysplasia, reactive endocervical cells from glandular dysplasia, and squamous from glandular differentiation in highgrade lesions.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value, feasibility, and validity of preparing cell blocks from fluid-based gynecologic cytology specimens

Keyhani‐Rofagha

Vesey-Shecket

2002

Cancer

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“…4,5 Cell blocks prepared from residual tissue fluids and fineneedle aspirations can be useful adjuncts to smears for establishing a more definitive cytopathological diagnosis and they can be a valuable diagnostic adjunct in cytological preparation in selected fluid-based cervicovaginal specimens. 6 Objectives of the Study The Aims and Objectives of the Study are-1. To study cytomorphology of Pap smears using the manual Liquid-based cytology (MLBC) and conventional Pap smear method.…”

mentioning

confidence: 99%

Advantages of Manual Liquid-Based Cytology in Cervicovaginal Smears With Special Emphasis on Cell Block

Kulkarni¹,

Manoli²,

Manoli³

2017

jemds

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Cell Block Preparation as a Diagnostic Technique Complementary to Fluid-Based Monolayer Cervicovaginal Specimens

Cited by 43 publications

References 8 publications

Manual Liquid Based Cytology in Primary Screening for Cervical Cancer - a Cost Effective Preposition for Scarce Resource Settings

Manual Liquid Based Cytology in Primary Screening for Cervical Cancer - a Cost Effective Preposition for Scarce Resource Settings

Diagnostic value, feasibility, and validity of preparing cell blocks from fluid-based gynecologic cytology specimens

Advantages of Manual Liquid-Based Cytology in Cervicovaginal Smears With Special Emphasis on Cell Block

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