Cell culture method for facilitating preparations for electron microscopy and replicate sampling using aclar fluoroplastic

Rowley, David R.; Feuchter, Frederick A.; HeidgerJr., Paul M.

doi:10.1007/bf00919790

Cited by 5 publications

(2 citation statements)

References 4 publications

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“…HeLa cells (5 ϫ 10 5 ) were seeded onto 6-well plates and incubated in 3 mL of medium with PLGAϪAu H-S NPs (30 g/cm 2 ) for ϳ4 to 12 h. Then, the cells were rinsed three times with phosphate buffered saline (PBS) and a cell pellet was collected using trypsin-EDTA and centrifugation. For TEM analysis, ultrathin (80 nm) sections of the cell pellet were prepared by a standard method, as described by D. R. Rowley et al 29 The samples were examined using TEM (JEM-1011, JEOL).…”

Section: Methodsmentioning

confidence: 99%

Multifunctional Nanoparticles for Combined Doxorubicin and Photothermal Treatments

et al. 2009

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To facilitate combined doxorubicin and photothermal treatments, we developed doxorubicin-loaded poly(lactic-co-glycolic acid)-gold half-shell nanoparticles (DOX-loaded PLGA-Au H-S NPs) by depositing Au films on DOX-loaded PLGA NPs. As the PLGA NPs biodegraded, DOX was released, and heat was locally generated upon near-infrared (NIR) irradiation due to NIR resonance of DOX-loaded PLGA H-S NPs. Compared with chemotherapy or photothermal treatment alone, the combined treatment demonstrated a synergistic effect, resulting in higher therapeutic efficacy and shorter treatment times. Since our NPs selectively deliver both heat and drug to tumorigenic regions, they may improve the therapeutic effectiveness with minimal side effects.

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Section: Methodsmentioning

confidence: 99%

Multifunctional Nanoparticles for Combined Doxorubicin and Photothermal Treatments

et al. 2009

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“…Ultrathin (80 nm) sections of the cell pellet were prepared by a standard method. [67] The samples were examined using TEM (Siemens Elmiskop 102 electron microscope, Siemens, Berlin, Germany).…”

Section: Dss-induced Colitis Model and Treatmentmentioning

confidence: 99%

GeTe Nanosheets as Theranostic Agents for Multimodal Imaging and Therapy of Inflammatory Bowel Disease

Song

Yoon

Lee

et al. 2021

Adv Funct Materials

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Recently, 2D nanomaterials have received considerable attention in nanomedi cine due to their intrinsic optical properties, biocompatibility, and therapeutic effect. Here, 2D germanium telluride (GeTe) nanosheets coated with polyvinyl pyrrolidone (PVP) (GeTePVP NSs) developed as theranostic agents are reported that can be used for multispectral optoacoustic tomography (MSOT) and fluorescence imaging and therapy of inflammatory bowel disease (IBD). GeTePVP NSs, fabricated by simple sonication in ethanol and PVP, show broad optical absorption in visible and nearinfrared (NIR) ranges (400-1200 nm) and have intrinsic fluorescence properties. Thus, they provide deeper ex vivo and in vivo MSOT tissue images than gold nanorods or indocyanine green with strong optical absorption in the first NIR window (700-900 nm). In addition, when orally administered to IBD mice, GeTePVP NSs exhibit therapeutic efficacy similar to or higher than sulfasalazine, with strong accumulation at inflamma tory colon sites. This demonstrates that oral administration of GeTePVP NSs may be used to treat inflammatory diseases in the gastrointestinal tract.

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Characterization of the heterogeneity of R3327 rat prostatic tumors derived from single‐cell clones

et al. 1985

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Prostatic adenocarcinoma is characterized by cellular diversity, which is well demonstrated in the Dunning R3327 rat prostatic adenocarcinoma. This heterogeneity may arise from epigenetic influences, ie, cellular adaptation or selection, and/or from genetic changes. To investigate the question of genetic instability, four tissue culture cell lines were derived from single cells isolated from the uncloned late (UCL) passage of the Dunning R3327H prostate cell culture. Each of these clonally derived tissue cultures was injected into castrated and intact young adult male rats for tumor production. Uncloned early (UCE) and UCL passage tissue cultures were also propagated as solid tumors. Tumors and the cultures from which they were derived were examined for evidence of phenotypic and genetic changes using morphological and cytometric methods. Transmission and scanning electron microscopy revealed only slight differences among the cell cultures. A single population of diploid cells was demonstrated in each of the cell cultures by propidium iodide staining and subsequent flow cytometric measurement of DNA content/nucleus. Tumors of unicellular as well as multicellular origin exhibited extreme heterogeneity of histological features, both among animals as well as within a single tumor. Tumors were surveyed and tissue types were characterized and cataloged. Clone 3 was generally better differentiated than the others; tumors from castrated animals were better differentiated than those from intact animals. Flow cytometry revealed multiple hyperdiploid cell populations that were variable from one sample to another. We concluded that changes in genotype as well as phenotype occurred in the tumors derived from single cells. Some of these changes may have occurred in the cells while still in culture.

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Cell culture method for facilitating preparations for electron microscopy and replicate sampling using aclar fluoroplastic

Cited by 5 publications

References 4 publications

Multifunctional Nanoparticles for Combined Doxorubicin and Photothermal Treatments

Multifunctional Nanoparticles for Combined Doxorubicin and Photothermal Treatments

GeTe Nanosheets as Theranostic Agents for Multimodal Imaging and Therapy of Inflammatory Bowel Disease

Characterization of the heterogeneity of R3327 rat prostatic tumors derived from single‐cell clones

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