Cell culture studies with the IMC-Hz-1 nonoccluded virus

1986

SUMMARYAn isolate of the non-occluded baculovirus Hz-1, containing a high level of defective particles, was recovered after serial passage in Trichoplusia ni (TN-368) tissue culture cells. DNA from defective virions contained deletions of up to 91 kilobase pairs. Defective particles were shown to interfere with the infection or replication of standard virions. Standard virus appeared to be required for replication of defective interfering (DI) particles. Initiation of both standard and DI viral DNA replication occurred at 4 h post-inoculation. Virus-induced protein synthesis was studied by pulse-labelling with [3SS]methionine. During defective virus replication in the presence of low amounts of standard virus, there was a significant reduction in the synthesis of 14 proteins (mol. wt. 109, 93, 92, 90, 85, 69, 57, 50, 40, 27.5, 23, 17.5, 17 and 14, all x 10 -3) and an increase in the synthesis of five proteins (mol. wt. 104, 75, 41, 37 and 14.2, all × 10 -3) as compared to standard virus infections.

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Section: Introductionmentioning

confidence: 99%

Intracellular Protein Synthesis During Standard and Defective Hz-1 Virus Replication

Burand

1986

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“…Kelly et al (1981) reported virus induction in IMC-HZ-1 cells following superinfection with other baculoviruses as well as u.v.-inactivated virus. Recently, Ralston et al (1981) have shown that this virus can establish a persistent infection in TN-368 cells. They suggested that defective interfering (DI) particles were involved in the persistence of HZ-1 virus; however, no such particles were identified.…”

Section: Introductionmentioning

confidence: 99%

Defective Particles from a Persistent Baculovirus Infection in Trichoplusia ni Tissue Culture Cells

Burand

Summers

1983

SUMMARYAn HZ-1 baculovirus clone isolated from persistently infected Trichoplusia ni tissue culture cells was homogeneous as determined by particle morphology and restriction enzyme analysis of virus DNA. Re-establishment of a persistent infection of TN-368 cells with this clone (B1) was unsuccessful. There was a 99~o decrease in infectious virus titre following 10 passages in tissue culture at a high multiplicity of infection, and the 10th passage virus population was capable of establishing a persistently infected cell line (TN-B1). Based on particle morphology, restriction enzyme analysis, and focus-forming plaque assays, the virus recovered from TN-B1 cells included a high percentage of defective virus particles which did not interfere with the infection of second-passage BI virus particles. The role of defective HZ-1 virus particles in the establishment of persistent infections is discussed.

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“…The Hz-1 virus is a non-occluded baculovirus (subgroup C of Baculoviridae), and was originally isolated from the persistently infected IMC-Hz-1 cell line established from ovarian tissue of Heliothis zea (Granados et al, 1978;Ralston et al, 1981). The Hz-1 virus can establish persistent and productive infections in several lepidopteran cell lines .…”

Section: Introductionmentioning

confidence: 99%

Physical map of Hz-1 baculovirus genome from standard and defective interfering particles

Chao¹,

Hamblin

1990