Physical map of Hz-1 baculovirus genome from standard and defective interfering particles

Chao, Yu-Chan; Hamblin, Martha T.; Wood, Helen

doi:10.1099/0022-1317-71-6-1265

Cited by 18 publications

(29 citation statements)

References 19 publications

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“…Alternatively, the differences in the PAT1 expression level may be due to variations resulting from different experiments, such as differences in natural mutations of the pag1 promoter or viral genes which interact with pag1 expression. Different Hz-1 viral genomic changes, mainly genomic deletions, were observed previously during persistent infection (Chao et al, 1990).…”

Section: Discussionmentioning

confidence: 94%

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Apoptosis resulting from superinfection of Heliothis zea virus 1 is inhibited by p35 and is not required for virus interference.

Lee

Chao

1998

Journal of General Virology

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Superinfection of Spodoptera frugiperda insect cells that are persistently infected with Heliothis zea 1 (Hz-1) virus induces general cellular apoptosis and subsequently results in homologous virus interference. Since apoptosis correlates closely with both a significant decrease in yield of virus progeny and expansion of virus infection among cells, further experiments were designed to verify the direct association of apoptosis with homologous interference. It was found that superinfection-induced apoptosis can be efficiently blocked by the stable transfection of p35 into cells before or after the

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Section: Discussionmentioning

confidence: 94%

“…This 2n9 kb RNA species was named the persistenceassociated transcript 1 (PAT1) and was transcribed by the persistence-associated gene 1 (pag1). PAT1 is a non-proteinencoding nuclear RNA with a function related to the establishment of persistent virus infection (Chao et al, 1990(Chao et al, , 1992(Chao et al, , 1998.…”

mentioning

confidence: 99%

Apoptosis resulting from superinfection of Heliothis zea virus 1 is inhibited by p35 and is not required for virus interference.

Lee

Chao

1998

Journal of General Virology

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“…2). The first ORF, Hz1V001, was defined as the first ORF present in the A fragment of XhoI-digested Hz-1 virus genome (13). The locations, orientations, sizes, and BLAST results of the ORFs are shown in Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…It contains a circular double-stranded DNA genome of 228 kb with a molecular mass of 131 ϫ 10 6 Da to 140 ϫ 10 6 Da (13,34). Hz-1 virus was classified as a member of subgroup C of the Baculoviridae and is a Nudibaculoviridae species.…”

mentioning

confidence: 99%

Analysis of the Complete Genome Sequence of the Hz-1 Virus Suggests that It Is Related to Members of the Baculoviridae

Cheng

Liu²,

Chow³

et al. 2002

J Virol

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We report the complete sequence of a large rod-shaped DNA virus, called the Hz-1 virus. This virus persistently infects the Heliothis zea cell lines. The Hz-1 virus has a double-stranded circular DNA genome of 228,089 bp encoding 154 open reading frames (ORFs) and also expresses a persistence-associated transcript 1, PAT1. The G؉C content of the Hz-1 virus genome is 41.8%, with a gene density of one gene per 1.47 kb. Sequence analysis revealed that a 9.6-kb region at 43.6 to 47.8 map units harbors five cellular genes encoding proteins with homology to dUTP pyrophosphatase, matrix metalloproteinase, deoxynucleoside kinase, glycine hydroxymethyltransferase, and ribonucleotide reductase large subunit. Other cellular homologs were also detected dispersed in the viral genome. Several baculovirus homologs were detected in the Hz-1 virus genome. These include PxOrf-70, PxOrf-29, AcOrf-81, AcOrf-96, AcOrf-22, VLF-1, RNA polymerase LEF-8 (orf50), and two structural proteins, p74 and p91. The Hz-1 virus p74 homolog shows high structural conservation with a double transmembrane domain at its C terminus. Phylogenetic analysis of the p74 revealed that the Hz-1 virus is evolutionarily distant from the baculoviruses. Another distinctive feature of the Hz-1 virus genome is a gene that is involved in insect development. However, the remainder of the ORFs (81%) encoded proteins that bear no homology to any known proteins. In conclusion, the sequence differences between the Hz-1 virus and the baculoviruses outnumber the similarities and suggest that the Hz-1 virus may form a new family of viruses distantly related to the Baculoviridae.

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“…The Hz-1 virus is a rod-shaped enveloped nonoccluded virus with a circular double-stranded DNA with genome size of approximately 228 kb (Huang et al 1982;Chao et al 1990). It was once classified as a member of subgroup C in the family Baculoviridae (Bilimoria 1986).…”

Section: Introductionmentioning

confidence: 98%

In vitro host range of the Hz-1 nonoccluded virus in insect cell lines

McIntosh

Grasela

Ignoffo

2007

In Vitro Cell.Dev.Biol.-Animal

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A total of 13 insect cell lines spanning 4 orders (Lepidoptera, Coleoptera, Diptera, and Homoptera) were tested for their ability to replicate the nonoccluded virus Hz-1. Only the Lepidopteran cell lines supported replication of the virus with TN-CL1 and BCIRL-HZ-AM1 producing the highest titers of 2.4 x 10(8) tissue culture infective dose (TCID)50/ml and 2.0 x 10(8) TCID50/ml, respectively. A codling moth cell line (CP-169) was the only Lepidopteran cell line that did not replicate the virus and transfection of this cell line with Hz-1 DNA failed to replicate the virus. Also, transfection with DNA from a recombinant baculovirus carrying the red fluorescent protein gene (AcMNPVhsp70 Red) was not expressed in CP-169 cells. The replication cycle of Hz-1 in BCIRL-HZ-AM1 cells showed that this virus replicated rapidly starting at 16 h postinoculation (p.i.) and reaching a peak titer of 1.0 x 10(8) TCID50/ml 56 h postinoculation. Hz-1 when compared with several other baculoviruses has the widest in vitro host spectrum.

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Physical map of Hz-1 baculovirus genome from standard and defective interfering particles

Cited by 18 publications

References 19 publications

Apoptosis resulting from superinfection of Heliothis zea virus 1 is inhibited by p35 and is not required for virus interference.

Apoptosis resulting from superinfection of Heliothis zea virus 1 is inhibited by p35 and is not required for virus interference.

Analysis of the Complete Genome Sequence of the Hz-1 Virus Suggests that It Is Related to Members of the Baculoviridae

In vitro host range of the Hz-1 nonoccluded virus in insect cell lines

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