Cell-Laden Thermosensitive Chitosan Hydrogel Bioinks for 3D Bioprinting Applications

Ku, Jongbeom; Seonwoo, Hoon; Park, Sangbae; Jang, Kitae; Lee, Juo; Lee, Myung Chul; Lim, Jae Woon; Kim, Jangho; Chung, Jong Hoon

doi:10.3390/app10072455

Cited by 34 publications

(17 citation statements)

References 34 publications

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“…Adhikari et al [ 28 ] developed a CS/alginate 3D printable bioink reinforced with hydroxyapatite, combining both pre- and post-printing crosslinking. Moreover, some studies reported the use of a support frame, made from synthetic thermoplastic polymer, to improve the shape of the CS constructs [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Preliminary Evaluation of 3D Printed Chitosan/Pectin Constructs for Biomedical Applications

et al. 2021

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In the present study, chitosan (CS) and pectin (PEC) were utilized for the preparation of 3D printable inks through pneumatic extrusion for biomedical applications. CS is a polysaccharide with beneficial properties; however, its printing behavior is not satisfying, rendering the addition of a thickening agent necessary, i.e., PEC. The influence of PEC in the prepared inks was assessed through rheological measurements, altering the viscosity of the inks to be suitable for 3D printing. 3D printing conditions were optimized and the effect of different drying procedures, along with the presence or absence of a gelating agent on the CS-PEC printed scaffolds were assessed. The mean pore size along with the average filament diameter were measured through SEM micrographs. Interactions among the characteristic groups of the two polymers were evident through FTIR spectra. Swelling and hydrolysis measurements confirmed the influence of gelation and drying procedure on the subsequent behavior of the scaffolds. Ascribed to the beneficial pore size and swelling behavior, fibroblasts were able to survive upon exposure to the ungelated scaffolds.

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Section: Introductionmentioning

confidence: 99%

Preliminary Evaluation of 3D Printed Chitosan/Pectin Constructs for Biomedical Applications

et al. 2021

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“…The unreacted –NH 2 groups of chitosan for such hydrogels are responsible for the material’s response to environmental pH variation. More recently, hydrogels of chitosan [ 32 ] and starch [ 33 ] were prepared by dissolution/melt-blending in carboxylic acids (i.e., acetic and citric acids) for applications in 3D bio-printing. Formation of single-component starch-based hydrogels are known to involve gelatinization, followed by a retrogradation process to form a 3D network [ 34 ].…”

Section: Fabrication Of Bio-based Hydrogelsmentioning

confidence: 99%

A Review on the Design and Hydration Properties of Natural Polymer-Based Hydrogels

2021

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Hydrogels are hydrophilic 3D networks that are able to ingest large amounts of water or biological fluids, and are potential candidates for biosensors, drug delivery vectors, energy harvester devices, and carriers or matrices for cells in tissue engineering. Natural polymers, e.g., cellulose, chitosan and starch, have excellent properties that afford fabrication of advanced hydrogel materials for biomedical applications: biodegradability, biocompatibility, non-toxicity, hydrophilicity, thermal and chemical stability, and the high capacity for swelling induced by facile synthetic modification, among other physicochemical properties. Hydrogels require variable time to reach an equilibrium swelling due to the variable diffusion rates of water sorption, capillary action, and other modalities. In this study, the nature, transport kinetics, and the role of water in the formation and structural stability of various types of hydrogels comprised of natural polymers are reviewed. Since water is an integral part of hydrogels that constitute a substantive portion of its composition, there is a need to obtain an improved understanding of the role of hydration in the structure, degree of swelling and the mechanical stability of such biomaterial hydrogels. The capacity of the polymer chains to swell in an aqueous solvent can be expressed by the rubber elasticity theory and other thermodynamic contributions; whereas the rate of water diffusion can be driven either by concentration gradient or chemical potential. An overview of fabrication strategies for various types of hydrogels is presented as well as their responsiveness to external stimuli, along with their potential utility in diverse and novel applications. This review aims to shed light on the role of hydration to the structure and function of hydrogels. In turn, this review will further contribute to the development of advanced materials, such as “injectable hydrogels” and super-adsorbents for applications in the field of environmental science and biomedicine.

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Section: Materials and Biomaterials For 3d‐printable Hydrogels For Bomentioning

confidence: 99%

Hydrogel‐Based 3D Bioprinting for Bone and Cartilage Tissue Engineering

Abdollahiyan

Oroojalian

Mokhtarzadeh

et al. 2020

Biotechnology Journal

112

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As a milestone in soft and hard tissue engineering, a precise control over the micropatterns of scaffolds has lightened new opportunities for the recapitulation of native body organs through three dimentional (3D) bioprinting approaches. Well-printable bioinks are prerequisites for the bioprinting of tissues/organs where hydrogels play a critical role. Despite the outstanding developments in 3D engineered microstructures, current printer devices suffer from the risk of exposing loaded living agents to mechanical (nozzle-based) and thermal (nozzle-free) stresses. Thus, tuning the rheological, physical, and mechanical properties of hydrogels is a promising solution to address these issues. The relationship between the mechanical characteristics of hydrogels and their printability is important to control printing quality and fidelity. Recent developments in defining this relationship have highlighted the decisive role of main additive manufacturing strategies. These strategies are applied to enhance the printing quality of scaffolds and determine the nurture of cellular morphology. In this regard, it is beneficial to use external and internal stabilization, photocurable biopolymers, and cooling substrates containing the printed scaffolds. The objective of this study is to review cutting-edge developments in hydrogel-type bioinks and discuss the optimum simulation of the zonal stratification in osteochondral and cartilage units.

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Cell-Laden Thermosensitive Chitosan Hydrogel Bioinks for 3D Bioprinting Applications

Cited by 34 publications

References 34 publications

Preliminary Evaluation of 3D Printed Chitosan/Pectin Constructs for Biomedical Applications

Preliminary Evaluation of 3D Printed Chitosan/Pectin Constructs for Biomedical Applications

A Review on the Design and Hydration Properties of Natural Polymer-Based Hydrogels

Hydrogel‐Based 3D Bioprinting for Bone and Cartilage Tissue Engineering

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