Cell length growth patterns in fission yeast reveal a novel size control mechanism operating in late G2 phase

Medgyes-Horváth, Anna; Rácz-Mónus, Anna; Buchwald, Péter

doi:10.1111/boc.201500066

Cited by 13 publications

(35 citation statements)

References 71 publications

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“…By visual inspection, some of the growth curves look exponential, some bilinear, and others have more complicated shapes ( Figure S2), as previously observed (Horváth et al, 2016). To demonstrate the heterogeneity of growth curve shapes, we fit exponential and bilinear curves to two cells representative of each shape ( Figure 2G,H).…”

Section: Wild-type Cells Grow Approximately Exponentiallysupporting

confidence: 59%

“…Fission yeast is an excellent system in which to study cell growth rate because its cylindrical cells grow only by elongation, making growth easy to measure microscopically (Mitchison, 1957). Many groups have taken advantage of this property to measure the growth kinetics of single fission yeast cells by time-lapse microscopy (Mitchison, 1957;Mitchison and Nurse, 1985;Miyata et al, 1988;Sveiczer et al, 1996;Baumgartner and Tolic-Norrelykke, 2009;Horvath et al, 2013;Horváth et al, 2016). Although fission yeast growth kinetics have been reported by several groups to be bilinear, reanalysis of some of the same data has led to the conclusion that growth is exponential (Mitchison and Nurse, 1985;Sveiczer et al, 1996;Cooper, 1998;Baumgartner and Tolic-Norrelykke, 2009;Cooper, 2013).…”

Section: Discussionmentioning

confidence: 99%

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Fission Yeast Cells Grow Approximately Exponentially

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Hollis

D’Souza

et al. 2018

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How the rate of cell growth is influenced by cell size is a fundamental question of cell biology. The simple model that cell growth is proportional to cell size, based on the proposition that larger cells have proportionally greater synthetic capacity than smaller cells, leads to the predication that the rate of cell growth increases exponentially with cell size. However, other modes of cell growth, including bilinear growth, have been reported. The distinction between exponential and bilinear growth has been explored in particular detail in the fission yeast Schizosaccharomyces pombe. We have revisited the mode of fission yeast cell growth using high-resolution time-lapse microscopy and find, as previously reported, that these two growth models are difficult to distinguish both because of the similarity in shapes between exponential and bilinear curves over the two-fold change in length of a normal cell cycle and because of the substantial biological and experimental noise inherent to these experiments. Therefore, we contrived to have cells grow more than two fold, by holding them in G2 for up to eight hours. Over this extended growth period, in which cells grow up to 5.5-fold, the two growth models diverge to the point that we can confidently exclude bilinear growth as a general model for fission yeast growth. Although the growth we observe is clearly more complicated than predicted by simple exponential growth, we find that exponential growth is a robust approximation of fission yeast growth, both during an unperturbed cell cycle and during extended periods of growth.

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Section: Wild-type Cells Grow Approximately Exponentiallysupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Fission Yeast Cells Grow Approximately Exponentially

Pickering

Hollis

D’Souza

et al. 2018

Preprint

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“…By visual inspection, some of the growth curves look exponential, some bilinear, and others have more complicated shapes ( Figure S3), as previously observed [29]. To demonstrate the heterogeneity of growth curve shapes, we fit exponential and bilinear curves to two cells representative of each shape ( Figure 2(g,h)).…”

Section: Wild-type Cells Grow Approximately Exponentiallysupporting

confidence: 56%

Fission yeast cells grow approximately exponentially

et al. 2019

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How the rate of cell growth is influenced by cell size is a fundamental question of cell biology. The simple model that cell growth is proportional to cell size, based on the proposition that larger cells have proportionally greater synthetic capacity than smaller cells, leads to the prediction that the rate of cell growth increases exponentially with cell size. However, other modes of cell growth, including bilinear growth, have been reported. The distinction between exponential and bilinear growth has been explored in particular detail in the fission yeast Schizosaccharomyces pombe. We have revisited the mode of fission yeast cell growth using high-resolution time-lapse microscopy and find, as previously reported, that these two growth models are difficult to distinguish both because of the similarity in shapes between exponential and bilinear curves over the twofold change in length of a normal cell cycle and because of the substantial biological and experimental noise inherent to these experiments. Therefore, we contrived to have cells grow more than twofold, by holding them in G2 for up to 8 h. Over this extended growth period, in which cells grow up to 5.5-fold, the two growth models diverge to the point that we can confidently exclude bilinear growth as a general model for fission yeast growth. Although the growth we observe is clearly more complicated than predicted by simple exponential growth, we find that exponential growth is a robust approximation of fission yeast growth, both during an unperturbed cell cycle and during extended periods of growth.

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“…In most cases, growth has been found to be exponential in bacteria and budding yeast (Godin et al 2010;Wang et al 2010;Osella et al 2014;Iyer-Biswas et al 2014;Di Talia et al 2007;) and bilinear in fission yeast (Horvath et al 2016;Sveiczer et al 1996;Mitchison 2003;Nobs & Maerkl 2014). Importantly, in all these organisms, size homeostasis has been reported to rely on an adaptation of cell cycle duration to initial size (reviewed in (Turner, Ewald, and Skotheim 2012;Osella et al 2017;Jorgensen and Tyers 2004).…”

Section: Introductionmentioning

confidence: 99%

Size control in mammalian cells involves modulation of both growth rate and cell cycle duration

Cadart

Monnier

Grilli

et al. 2017

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SummaryDespite decades of research, it remains unclear how mammalian cell growth varies with cell size and across the cell division cycle to maintain size control. Answers have been limited by the difficulty of directly measuring growth at the single cell level. Here we report direct measurement of single cell volumes over complete cell division cycles. The volume added across the cell cycle was independent of cell birth size, a size homeostasis behavior called "adder". Single-cell growth curves revealed that the homeostatic behavior relied on adaptation of G1 duration as well as growth rate modulations. We developed a general mathematical framework that characterizes size homeostasis behaviors. Applying it on datasets ranging from bacteria to mammalian cells revealed that a near-adder is the most common type of size control, but only mammalian cells achieve it using modulation of both cell growth rate and cell-cycle progression.

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Cell length growth patterns in fission yeast reveal a novel size control mechanism operating in late G2 phase

Cited by 13 publications

References 71 publications

Fission Yeast Cells Grow Approximately Exponentially

Fission Yeast Cells Grow Approximately Exponentially

Fission yeast cells grow approximately exponentially

Size control in mammalian cells involves modulation of both growth rate and cell cycle duration

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