Cell recycling studies for ?-amylase production by Bacillus amyloliquefaciens

Overproduction of proteinase A by recombinant Saccharomyces cerevisiae was investigated by cultivations in a cell-recycling bioreactor. Membrane filtration was used to separate cells from the broth. Recycling ratios and dilution rates were varied and the effect on enzyme production was studied both experimentally and by computer simulations. Experiments and simulations showed that cell mass and product concentration were enhanced by high ratios of recycling. Additional simulations showed that the proteinase A concentration decreased drastically at high dilution rates and the optimal volumetric productivities were at high dilution rates just below washout and at high ratios of recycling. Cell-recycling fermentation gave much higher volumetric productivities and stable product concentrations in contrast to simple continuous fermentation.

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Production of proteinase A bySaccharomyces cerevesiae in a cell-recycling fermentation system: experiments and computer simulations

Grøn

Biedermann

Emborg

1996

Appl Microbiol Biotechnol

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A Two-Stage System for the Large-Scale Cultivation of Biomass: a Design and Operation Analysis Based on a Simple Steady-State Model Tuned on Laboratory Measurements

Silva

Bertucco

2018

Bioenerg. Res.

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Mathematical Modeling of Proteinase A Overproduction by Saccharomyces cerevisiae

Grøn

Jochumsen

Biedermann

et al. 1996

Annals of the New York Academy of Sciences

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A simple, structured model was developed to describe the growth and product formation behavior of two recombinant strains of Saccharomyces cerevisiae (JG176 and JG180), both overproducing extracellular proteinase A. The model parameters were estimated to data from continuous fermentations obtained at steady-state conditions. Model predictions show good agreement with experimental data obtained by batch fermentations. The two concerned organisms are distinguished from each other by the type of promoter on the plasmids controlling the proteinase A expression. The proteinase A transcription is controlled by the natural proteinase A promoter in JG176 and by a tpi promoter in JG180. By means of experiments and simulations, the extracellular product formation from the two strains with different promoter systems was compared in batch and continuous fermentations. The results showed that the proteinase A formation kinetic from JG176 was a combination of growth and nongrowth associated (production in the stationary growth phase), whereas the proteinase A formation from JG180 was truly growth associated (production in the exponential growth phase). In both batch and continuous cultivations JG176 gave the highest product concentrations and volumetric productivities.

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Cell recycling studies for ?-amylase production by Bacillus amyloliquefaciens

Cited by 3 publications

References 11 publications

Production of proteinase A bySaccharomyces cerevesiae in a cell-recycling fermentation system: experiments and computer simulations

Production of proteinase A bySaccharomyces cerevesiae in a cell-recycling fermentation system: experiments and computer simulations

A Two-Stage System for the Large-Scale Cultivation of Biomass: a Design and Operation Analysis Based on a Simple Steady-State Model Tuned on Laboratory Measurements

Mathematical Modeling of Proteinase A Overproduction by Saccharomyces cerevisiae

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