Cell surface physiology and outer cell envelope impermeability for hydrophobic substances in Burkholderia multivorans

Ruskoski, Sallie A.; Champlin, Franklin R.

doi:10.1099/jmm.0.000532

Cited by 5 publications

(7 citation statements)

References 29 publications

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“…Similar to observations with Bpc organisms, the serum-sensitivity profile of Bcc isolates is dependent on both the expression of a bacterial capsule and the LPS O-PS ( Butler et al., 1994 ; Su et al., 2010 ; Ruskoski and Champlin, 2017 ). A notable difference, however, is that expression of these virulence factors varies considerably even within each species ( Figure 1 ).…”

Section: Burkholderia and Complementsupporting

confidence: 65%

“…The O-antigenic polysaccharide (O-PS) of Bcc strains are distinct from those expressed by Bpc strains in that there is no strain-specific consistency among them due to the selective pressure environmental conditions put on the Bcc O-PS gene cluster ( Figure 1 ) ( Butler et al., 1994 ; Chung et al., 2003 ; Hassan et al., 2017 ; Ruskoski and Champlin, 2017 ). Strains expressing full-length O-PS are sometimes described in the literature as having “smooth LPS”, and strains expressing truncated or no O-PS are described as having “rough LPS” ( Butler et al., 1994 ).…”

Section: Lipopolysaccharide and Capsulementioning

confidence: 99%

“…Expression of capsular polysaccharide by Bcc isolates has been suggested to influence strain virulence and, like the expression of O-PS, has been demonstrated to depend on environmental cues ( Chung et al., 2003 ; Ruskoski and Champlin, 2017 ). Isolates expressing capsule are described in the literature as being “mucoid”, while isolates lacking capsule are described as “non-mucoid” ( Cerantola et al., 2000 ; Ruskoski and Champlin, 2017 ).…”

Section: Lipopolysaccharide and Capsulementioning

confidence: 99%

“…A notable difference, however, is that expression of these virulence factors varies considerably even within each species ( Figure 1 ). Indeed, the expression of both capsule and O-PS by Bcc isolates are significantly modulated by changes in the extracellular environment ( Su et al., 2010 ; Ruskoski and Champlin, 2017 ). While the expression of capsule can influence serum survival, the major determinant of serum resistance in Bcc isolates remains the O-PS.…”

Section: Burkholderia and Complementmentioning

confidence: 99%

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Interactions Between Pathogenic Burkholderia and the Complement System: A Review of Potential Immune Evasion Mechanisms

Syed

Wooten

2021

Front. Cell. Infect. Microbiol.

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The genus Burkholderia contains over 80 different Gram-negative species including both plant and human pathogens, the latter of which can be classified into one of two groups: the Burkholderia pseudomallei complex (Bpc) or the Burkholderia cepacia complex (Bcc). Bpc pathogens Burkholderia pseudomallei and Burkholderia mallei are highly virulent, and both have considerable potential for use as Tier 1 bioterrorism agents; thus there is great interest in the development of novel vaccines and therapeutics for the prevention and treatment of these infections. While Bcc pathogens Burkholderia cenocepacia, Burkholderia multivorans, and Burkholderia cepacia are not considered bioterror threats, the incredible impact these infections have on the cystic fibrosis community inspires a similar demand for vaccines and therapeutics for the prevention and treatment of these infections as well. Understanding how these pathogens interact with and evade the host immune system will help uncover novel therapeutic targets within these organisms. Given the important role of the complement system in the clearance of bacterial pathogens, this arm of the immune response must be efficiently evaded for successful infection to occur. In this review, we will introduce the Burkholderia species to be discussed, followed by a summary of the complement system and known mechanisms by which pathogens interact with this critical system to evade clearance within the host. We will conclude with a review of literature relating to the interactions between the herein discussed Burkholderia species and the host complement system, with the goal of highlighting areas in this field that warrant further investigation.

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Section: Burkholderia and Complementsupporting

confidence: 65%

Section: Lipopolysaccharide and Capsulementioning

confidence: 99%

Section: Lipopolysaccharide and Capsulementioning

confidence: 99%

Section: Burkholderia and Complementmentioning

confidence: 99%

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Interactions Between Pathogenic Burkholderia and the Complement System: A Review of Potential Immune Evasion Mechanisms

Syed

Wooten

2021

Front. Cell. Infect. Microbiol.

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“…multivorans are composed of similar phospholipid profiles, while their substituent fatty acyl groups differ quantitatively [ 18 ]. Moreover, expression of extracellular polysaccharide does not influence the degree to which they associate with nonpolar substances [ 19 ]. The present study was undertaken to determine if B .…”

Section: Introductionmentioning

confidence: 99%

Disparate properties of Burkholderia multivorans and Pseudomonas aeruginosa regarding outer membrane chemical permeabilization to the hydrophobic substances novobiocin and triclosan

et al. 2023

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Burkholderia multivorans causes opportunistic pulmonary infections and is intrinsically resistant to many antibacterial compounds including the hydrophobic biocide triclosan. Chemical permeabilization of the Pseudomonas aeruginosa outer membrane affects sensitization to hydrophobic substances. The purpose of the present study was to determine if B. multivorans is similarly susceptive suggesting that outer membrane impermeability properties underlie triclosan resistance. Antibiograms and conventional macrobroth dilution bioassays were employed to establish baseline susceptibility levels to hydrophobic antibacterial compounds. Outer membrane permeabilizers compound 48/80, polymyxin B, polymyxin B-nonapeptide, and ethylenediaminetetraacetic acid were used in attempts to sensitize disparate B. multivorans isolates to the hydrophobic agents novobiocin and triclosan, and to potentiate partitioning of the hydrophobic fluorescent probe 1-N-phenylnapthylamine (NPN). The lipophilic agent resistance profiles for all B. multivorans strains were essentially the same as that of P. aeruginosa except that they were resistant to polymyxin B. Moreover, they resisted sensitization to hydrophobic compounds and remained inaccessible to NPN when treated with outer membrane permeabilizers. These data support the notion that while both phylogenetically-related organisms exhibit general intrinsic resistance properties to hydrophobic substances, the outer membrane of B. multivorans either resists permeabilization by chemical modification or sensitization is mitigated by a supplemental mechanism not present in P. aeruginosa.

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Influence of outer membrane permeabilization on intrinsic resistance to the hydrophobic biocide triclosan in opportunistic Serratia species

Boyina¹,

King²,

Rigsbee³

et al. 2023

Heliyon

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Cell surface physiology and outer cell envelope impermeability for hydrophobic substances in Burkholderia multivorans

Abstract: These data support the conclusion that EPS expression and the consistent mucoid phenotype are not necessarily associated with the ability of the outer cell surface to associate with non-polar substances or cellular capsulation.

Cited by 5 publications

References 29 publications

Interactions Between Pathogenic Burkholderia and the Complement System: A Review of Potential Immune Evasion Mechanisms

Interactions Between Pathogenic Burkholderia and the Complement System: A Review of Potential Immune Evasion Mechanisms

Disparate properties of Burkholderia multivorans and Pseudomonas aeruginosa regarding outer membrane chemical permeabilization to the hydrophobic substances novobiocin and triclosan

Influence of outer membrane permeabilization on intrinsic resistance to the hydrophobic biocide triclosan in opportunistic Serratia species

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