Cell tropism of Staphylococcus aureus in bovine mammary gland cell cultures

Lammers, A.; Nuijten, Piet J. M.; Kruijt, E.; Stockhofe-Zurwieden, Norbert; Vecht, U.; Smith, Hilde E.; Zijderveld, F.G. van

doi:10.1016/s0378-1135(99)00018-8

Cited by 25 publications

(19 citation statements)

References 22 publications

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“…Several studies have been reported about S. aureus adhesion to mammary epithelial cells [1]. Recently, we described the adhesion of S. aureus cells to a novel type of bovine mammary gland cells in vitro [3]. Cultures of alveolar cells contained two morphologically di¡erent cell types, which were most probably of myoepithelial and secretory epithe-lial origin.…”

Section: Introductionmentioning

confidence: 92%

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The fibronectin binding proteins of Staphylococcus aureus are required for adhesion to and invasion of bovine mammary gland cells

Lammers¹

1999

FEMS Microbiology Letters

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We recently described adhesion to and invasion of bovine mammary gland cells by Staphylococcus aureus in vitro. Here, we show that the levels of adhesion and invasion are dependent on the bacterial growth phase and are controlled by the agr locus. Incubation of exponential growth phase cells of S. aureus with mammary gland cells resulted in bacterial cell clumping. Strains of S. aureus deficient in expression of the fibronectin binding proteins (FnBPA and FnBPB) failed to clump and their ability to adhere to and to invade the bovine mammary gland cells is strongly reduced. This indicates that the fibronectin binding proteins are essential for S. aureus adhesion to and invasion of bovine mammary gland cells. ß

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Section: Introductionmentioning

confidence: 92%

“…S. aureus adhered almost exclusively to the putative myoepithelial cells. Moreover, the bacteria were internalized e¤ciently by these cells [3].…”

Section: Introductionmentioning

confidence: 99%

The fibronectin binding proteins of Staphylococcus aureus are required for adhesion to and invasion of bovine mammary gland cells

Lammers¹

1999

FEMS Microbiology Letters

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“…Most of mastitis pathogen adhesion studies have been performed with cell cultures for which several models have been described. Lammers et al [132] used E. coli strain HB101 as negative control to test adhesion and invasion of udder pathogens. In a recent study, the specificity and efficiency of adhesion of 11 different E. coli strains was studied.…”

Section: Adhesion and Colonisationmentioning

confidence: 99%

Severity of E. coli mastitis is mainly determined by cow factors

et al. 2003

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-Intramammary infections of dairy cows with Gram-positive bacteria such as Staphylococcus aureus (major cause of mastitis) have received a lot of attention because of their major economic impact on the dairy farm through production losses induced by an increase in somatic cell count. Management strategies, including greater awareness for efficient milking and hygienic measures, have limited the spread of Gram-positive bacteria and resulted in a significant decrease of proportion of S. aureus isolates and subclinical mastitis worldwide. Other organisms such as coliform subspecies and Streptococcus uberis, both environmental bacteria that cause clinical mastitis, have received less attention. Escherichia coli causes inflammation of the mammary gland in dairy cows around parturition and during early lactation with striking local and sometimes severe systemic clinical symptoms. This disease affects many high producing cows in dairy herds and may cause several cases of death per year in the most severe cases. It is well known that bacterial, cow and environmental factors are interdependent and influence mastitis susceptibility. Many studies, executed during the last decade, indicate that the severity of E. coli mastitis is mainly determined by cow factors rather than by E. coli pathogenicity. During E. coli mastitis, the host defense status is a cardinal factor determining the outcome of the disease. Today, we know that the neutrophil is a key factor in the cows' defense against intramammary infection with E. coli. Effective elimination of the pathogen by neutrophils is important for the resolution of infection and the outcome of E. coli mastitis. This review is a compilation of some major findings over the last 15 years concerning mainly host factors that modulate and influence neutrophil function and the mammary inflammatory reaction. The individual chapters address: virulence factors of E. coli strains, how neutrophils kill E. coli, connection between endotoxins, tumor necrosis factor-a and nitric oxide, severity classification of E. coli mastitis, lifespan of neutrophils, host factors that influence severity, tissue damage and production loss.

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“…Only a few mammary gland cell lines have been established for domestic species such as bovine (LAMMERS et al, 1999;MATITASHVILI;BAUMAN, 1999;ROSE et al, 2002;HU et al, 2009), swine (SUN et al, 2006, and ovine (DÜCHLER et al, 1998;ILAN et al, 1998). There are strong biological differences between rodent and ruminant lactation, mainly regarding milk composition and effect/response of regulatory factors on mammary cells (JENNESS; SLOAN, 1970;GREEN;PASTEWKA, 1976).…”

Section: Introductionmentioning

confidence: 99%

In vitro culture of ovine mammary gland cells expressing beta-lactoglobulin and beta-casein

Giassetti

Barros

Mendes

et al. 2017

Braz. J. Vet. Res. Anim. Sci.

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The expression of milk proteins in vitro is essential to exploit the mammary gland cells as a biological model. Enzymatic tissue disaggregation has been widely used to establish mammary cell culture, but its effect in long-term ovine mammary cell culture is not completely elucidated. This study aimed at comparing mechanical/enzymatic and mechanical dissociation methods to establish ovine mammary cell culture. We compared cellular differentiation induced by lactating ewe serum or fetal bovine serum based on the gene expression levels of milk proteins (beta-lactoglobulin, alpha s1-casein, and betacasein). Mechanically dissociated cells were positive immunostaining for cytokeratin 8.13, such as mammary epithelial cells. These cells are responsible for milk protein expression and they are low immunostaining for vimentin, mesenchymal marker. Mechanical/enzymatic dissociation cells were positive for vimentin. The fastest cell growth (cell/hour) was observed in the mechanical dissociation group cultured with 10% fetal bovine serum medium. Mechanically and mechanically/enzymatically derived cells were able to express beta-casein and beta-lactoglobulin, but not alpha s1-casein. The relative expression of beta-lactoglobulin was not affected by the tissue dissociation method or culture media, beta-casein relative expression was down regulated in mechanically dissociated cells cultured in the presence of lactating ewe serum, (P = 0.019). Beta-casein relative expression was also down regulated in mechanically/enzymatically dissociated cells cultured with fetal bovine serum (P = 0.021). In the present conditions, we conclude that mechanical dissociation followed by culture with 10% of fetal bovine serum was the most efficient method to induce milk proteins' mRNA expression by ovine mammary epithelial cells in vitro. Keywords: Mammary cell culture. Milk proteins. Beta-casein. Ovine. ResumoA expressão in vitro de proteínas do leite é essencial para explorar as células da glândula mamária como um modelo biológico. A desagregação tecidual via enzimática é amplamente utilizada para o estabelecimento cultivo de células mamárias. No entanto, seu efeito a longo prazo no cultivo de células da glândula mamária ovina ainda não é bem elucidado. Este estudo tem como objetivo comparar dois métodos de dissociação tecidual, mecânico/enzimático e mecânico, para estabelecer cultivo celular de glândula mamária ovina. A indução da diferenciação celular, por adição de soro de ovelha lactante ou soro fetal bovino, foi avaliada pelos níveis de expressão de proteínas do leite (beta-lactoglobulina, alpha s1-caseína e beta-caseína). Células mecanicamente dissociadas foram positivamente marcadas para a presença de citoqueratina 8.13, marcador para células epiteliais mamárias. Essas células são as responsáveis pela produção das proteínas do leite e são pouco marcadas para a presença de vimentina, marcador para células de origem mesenquimal. Já as células obtidas da dissociação mecânica/ enzimática foram positivamente marcadas para presença de vi...

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Cell tropism of Staphylococcus aureus in bovine mammary gland cell cultures

Cited by 25 publications

References 22 publications

The fibronectin binding proteins of Staphylococcus aureus are required for adhesion to and invasion of bovine mammary gland cells

The fibronectin binding proteins of Staphylococcus aureus are required for adhesion to and invasion of bovine mammary gland cells

Severity of E. coli mastitis is mainly determined by cow factors

In vitro culture of ovine mammary gland cells expressing beta-lactoglobulin and beta-casein

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