The fibronectin binding proteins of Staphylococcus aureus are required for adhesion to and invasion of bovine mammary gland cells

Lammers, A

doi:10.1016/s0378-1097(99)00470-x

Cited by 32 publications

(38 citation statements)

References 11 publications

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“…Genetic determinants of the FNBB and CNA were found in only a few isolates (Table 3) and thus their role in mastitis could not be clearly established. The present observations are in concurrences with previous reports (Lammers et al 1999;Salasia et al 2004;El-Sayed et al 2006).…”

Section: Discussionsupporting

confidence: 94%

Prevalence of adhesin and toxin genes among isolates of Staphylococcus aureus obtained from mastitic cattle

Kumar

Yadav

Anand

et al. 2010

World J Microbiol Biotechnol

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Staphylococcus aureus is recognized as a major etiological agent of the most important udder disease; mastitis. The virulence potential of S. aureus isolates is attributed to a combination of extracellular factors and invasive properties that are controlled at the genomic level. In this study molecular variations were studied among the 128 S. aureus isolates obtained from the mastitic crossbred cattle. The polymorphic patterns of protein A, coagulase, and IG genes were studied in the isolates using the PCR and PCR-RFLP methods. In addition, presence of other virulence genes responsible for the expression of adhesins and toxins was also screened. A considerable genetic variation was observed in amplified fragment of SPA, IG and CLF genes. The PCR-RFLP of COA gene yielded five different patterns. Occurrence of EBP, ENO and FNBA was found to be common in both high and low virulence isolates. However, the prevalence of FNBB and toxin genes was higher in clinical than subclinical isolates. The distribution proportions of adhesin genes EBP, FIB, FNBB, CNA, BBP, MAP CAP5, and CAP8 were 60.2, 59.4, 21.9, 6.3, 5.5, 80.5, 56.3, and 22.7%; for AGR I, II, III, and IV were 44.5, 32.8, 12.5, and 10.2%; and for toxins genes HLB, SEB, SEC, SED, SEE, SEG, and SEI were 82.0, 3.1, 5.5, 3.9, 0.8, 16.4, and 55.5%, respectively. The proportions of FNBB, EBP, FIB, HLB, BBP, SEG, and SEI genes were observed more in clinical cases. The significant genetic variations in SPA, COA, IG, and CLF genes were useful to differentiate the isolates that might be valuable for mastitis reduction programme.

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Section: Discussionsupporting

confidence: 94%

Prevalence of adhesin and toxin genes among isolates of Staphylococcus aureus obtained from mastitic cattle

Kumar

Yadav

Anand

et al. 2010

World J Microbiol Biotechnol

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“…It was reported elsewhere that an agr Ϫ mutant (RN6911) displayed enhanced adhesion and invasive properties [33]. Indeed, agr attenuation was verified in an epidemic MRSA clone (non-BECC C-MRSA-3) from Canada that also displayed increased Fn-binding activity [34].…”

Section: Discussionmentioning

confidence: 84%

The Predominant Variant of the Brazilian Epidemic Clonal Complex of Methicillin‐ResistantStaphylococcus aureusHas an Enhanced Ability to Produce Biofilm and to Adhere to and Invade Airway Epithelial Cells

et al. 2005

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Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a therapeutic problem. In the present study, the molecular characterization by pulsed-field gel electrophoresis of MRSA isolates collected from a university hospital revealed that the predominant variant of the Brazilian epidemic clonal complex (BECC) was responsible for the increase in the incidence of MRSA strains, which reached 28% in 1998. It was verified that this predominant variant of the BECC displayed an enhanced ability to produce biofilm on inert polystyrene surfaces and to adhere to and invade epithelial airway cells. These results indicate that MRSA strains belonging to the BECC have evolved advantageous properties that might play a role in their predominance as international nosocomial pathogens.

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“…aureus expresses a number of cell wall-anchored proteins [28,29], many of which act as adhesins for extracellular matrix proteins [30][31][32][33]. The importance of these MSCRAMMs (CNA, ClfA, FnbpA, and FnbpB) as virulence factors in S. aureus infections has been demonstrated in various studies [34][35][36][37][38][39][40][41][42][43][44][45][46].…”

Section: Discussionmentioning

confidence: 99%

Virulence Potential of the Staphylococcal Adhesin CNA in Experimental Arthritis Is Determined by Its Affinity for Collagen

et al. 2004

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The fibronectin binding proteins of Staphylococcus aureus are required for adhesion to and invasion of bovine mammary gland cells

Cited by 32 publications

References 11 publications

Prevalence of adhesin and toxin genes among isolates of Staphylococcus aureus obtained from mastitic cattle

Prevalence of adhesin and toxin genes among isolates of Staphylococcus aureus obtained from mastitic cattle

The Predominant Variant of the Brazilian Epidemic Clonal Complex of Methicillin‐ResistantStaphylococcus aureusHas an Enhanced Ability to Produce Biofilm and to Adhere to and Invade Airway Epithelial Cells

Virulence Potential of the Staphylococcal Adhesin CNA in Experimental Arthritis Is Determined by Its Affinity for Collagen

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