Cell viability, anti-proliferation and antioxidant activities of Sideritis syriaca, Tanacetum argenteum subsp. argenteum and Achillea aleppica subsp. zederbaueri on human breast cancer cell line (MCF-7)

Abstract

The breast cancer is one of the most common types of cancer. Many scientists have focused on the treatment of this disease for a long time by studying anti-cancer activities of plant extracts as well as synthetics. Lamiaceae and Asteraceae have been used in anticancer studies due to their phytochemical content. The genus Sideritis, Achillea and Tanacetumare the members of these families. Sideritis, Achillea and Tanacetum are used as herbal medication for the treatment ofvariety of diseases. In present study, we demonstrated the biological activity of Sideritis syriaca (SS), Achillea aleppica (AAZ) and Tanacetum argenteum (TAA) methanol extracts on cell viability of the breast cancer line MCF7. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to determine the cell viability and proliferation of MCF7 cells. In a dose dependent manner, methanol extracts (0, 1, 5, 25, 100 and 250 µg/ml) of SS, AZZ and TAA were examined on MCF7, and viability of cells were determined with MTT staining. Especially, concentrations in 100 and 250 µg/ml of extracts decreased the cell viability (p<0.001). The results of the current study showed that methanol extracts of SS, AZZ and TAA effectively inhibited the cell proliferation by decreasing the cell viability of MCF7 cells. Suggesting that SS, AZZ and TAA can be considered as natural herbal-based anti-cancerous agents.

Endemik Tanacetum argenteum subsp. argenteum bitkisinin toprak üstü kısımlarından elde edilen ekstrelerin biyolojik aktiviteleri

“…argenteum leaves inhibited growth of MCF-7 cancer cell line by 47.42% at the concentration of 100 µg/mL. This value was lower than the value in our present study (64.76%) (7). This difference could be explained based on the fact that different extraction method was used.…”

<i>In Vitro</i> Cytotoxic and Anti-inflammatory Activities of <i>Tanacetum argenteum</i> (Lam.) Willd. subsp. <i>argenteum</i> Extract

“…The difference is likely due to the extraction methods. 17 Recently, Şen et al 18 investigated the cytotoxic activity of aerial parts of T. argenteum subsp. argentum against four cell lines [A549, HeLa, HT-29 (human colorectal adenocarcinoma), MCF-7] using MTT assays.…”

Pharmacological properties and therapeutic potential of saffron (Crocus sativus L.) in osteosarcoma

“…Flowers of C. sativus L. were harvested early in the morning, and stigmas were separated and dried quickly. Standard Soxhlet extraction method was used for the preparation of plant extracts using methanol, dichloromethane and hexane as described previously …”

“…Standard Soxhlet extraction method was used for the preparation of plant extracts using methanol, dichloromethane and hexane as described previously. [13]…”