Cell wall growth during the cell cycle of <i>Schizosaccharomyces pombe</i>

Streiblová, Eva; Wolf, Amnon

doi:10.1002/jobm.19720120808

Cited by 28 publications

(17 citation statements)

References 8 publications

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“…During mid-G2, growth rate increases again by approximately 30% (RCP2; Figure 1B). This change in growth rate coincides with the switch from mono-polar growth (growth only at the old pole) to bi-polar growth (growth at both poles) [10,13], a transition that has been termed NETO (New-End-Take-Off). Curiously however, RCP2 does not depend on NETO.…”

Section: Growth Rate Changes At Multiple Points During the Cell Cyclementioning

confidence: 99%

Growth and division—not a one-way road

Goranov

Amon

2010

Current Opinion in Cell Biology

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SummaryMaintaining cell size homeostasis and regulating cell size in response to changing conditions is a fundamental property of organisms. Here we examine the recent advances in our understanding of the interplay between accumulation of mass (growth) and the progression through the cell cycle (proliferation), the coordination of which determines the size of cells. It is well established that growth affects cell division (reviewed in [1]). This review will focus on the reverse, less welldefined relationship -how cell cycle progression affects growth. We will summarize findings that indicate that growth is not constant during the cell cycle and discuss the surprising possibility that cyclin-dependent kinases (CDKs) inhibit growth.

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Section: Growth Rate Changes At Multiple Points During the Cell Cyclementioning

confidence: 99%

Growth and division—not a one-way road

Goranov

Amon

2010

Current Opinion in Cell Biology

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“…Approximately 300 viable cells were spread per one YPDP plate. Plates were incubated at 29°C for 4 d, and colonies were then replica plated on YPDP and incubated at 36°C for 1 d. Strains that did not form colonies or that formed sick dark-red colonies were picked, and the morphology of these cells was examined by Calcofluor staining (Streiblová and Wolf, 1972). Approximately 1 of 50 colonies showed either no growth or retarded growth at the restrictive temperature.…”

Section: Isolation Of Ts Mutants With Polarity Defectsmentioning

confidence: 99%

“…We first isolated ts mutants and then examined the cell morphology of these mutants after incubation at the restrictive temperature using Calcofluor, which stains septa and growing ends of the cell (Streiblová and Wolf, 1972;Mitchison and Nurse, 1985). Mutants with bent, curved, or branched morphology were selected.…”

Section: Isolation Of Mutants That Are Defective In Growth Polaritymentioning

confidence: 99%

Identification of Novel Temperature-sensitive Lethal Alleles in Essential β-Tubulin and Nonessential α2-Tubulin Genes as Fission Yeast Polarity Mutants

Radcliffe

Hirata

Childs

et al. 1998

MBoC

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We have screened for temperature-sensitive (ts) fission yeast mutants with altered polarity (alp1-15). Genetic analysis indicates that alp2 is allelic to atb2 (one of two ␣-tubulin genes) and alp12 to nda3 (the single ␤-tubulin gene). atb2 ϩ is nonessential, and the ts atb2 mutations we have isolated are dominant as expected. We sequenced two alleles of ts atb2 and one allele of ts nda3. In the ts atb2 mutants, the mutated residues (G246D and C356Y) are found at the longitudinal interface between ␣/␤-heterodimers, whereas in ts nda3 the mutated residue (Y422H) is situated in the domain located on the outer surface of the microtubule. The ts nda3 mutant is highly sensitive to altered gene dosage of atb2 ϩ

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“…Once a minimal cell length has been reached, the cells activate bipolar growth by initiating growth from the newly formed tips (New End Take Off [NETO]; Streiblova and Wolf, 1972;Nurse, 1975;Mitchison and Nurse, 1985). Polarized cell growth is strictly cell cycle regulated.…”

Section: Introductionmentioning

confidence: 99%

Regulation of Cell Diameter, For3p Localization, and Cell Symmetry by Fission Yeast Rho-GAP Rga4p

Das

Wiley

Medina

et al. 2007

MBoC

108

168

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Control of cellular dimensions and cell symmetry are critical for development and differentiation. Here we provide evidence that the putative Rho-GAP Rga4p of Schizosaccharomyces pombe controls cellular dimensions. rga4 Delta cells are wider in diameter and shorter in length, whereas Rga4p overexpression leads to reduced diameter of the growing cell tip. Consistent with a negative role in cell growth control, Rga4p protein localizes to the cell sides in a "corset" pattern, and to the nongrowing cell tips. Additionally, rga4 Delta cells show an altered growth pattern similar to that observed in mutants of the formin homology protein For3p. Consistent with these observations, Rga4p is required for normal localization of For3p and for normal distribution of the actin cytoskeleton. We show that different domains of the Rga4p protein mediate diverse morphological functions. The C-terminal GAP domain mediates For3p localization to the cell tips and maintains cell diameter. Conversely, overexpression of the N-terminal LIM homology domain of Rga4p promotes actin cable formation in a For3p-dependent manner. Our studies indicate that Rga4p functionally interacts with For3p and has a novel function in the control of cell diameter and cell growth.

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Cell wall growth during the cell cycle of Schizosaccharomyces pombe

Cited by 28 publications

References 8 publications

Growth and division—not a one-way road

Growth and division—not a one-way road

Identification of Novel Temperature-sensitive Lethal Alleles in Essential β-Tubulin and Nonessential α2-Tubulin Genes as Fission Yeast Polarity Mutants

Regulation of Cell Diameter, For3p Localization, and Cell Symmetry by Fission Yeast Rho-GAP Rga4p

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