Cellular And Dna Changes Due To Clonazepam Abuse In Brains Of Albino Rats And Role Of Clonidine During Withdrawal Period

Girgis, Naira F; Kamel, Sahar; Labib, Bothina; Naby, Sobhy El; Samy, S. Hadjadj

doi:10.21608/mjfmct.2010.53495

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study proved more toxic effect in the combined doses of tramadol and diazepam and more destruction of neuronal cells, a very weak PAS reaction was noted in the specimen of tramadol and diazepam dependent treated rats. This finding was similar to the study of Girgis et al, (2010). Marked expression of positive apoptotic cells with p53 stain in the combined abuse of tramadol and diazepam treated rats prove the histopathologic and histochemical alterations that were previously reported in this study that highlighted the fact that coadministration of both drugs enhances their toxic effects.…”

Section: Discussionsupporting

confidence: 92%

“…In the present study weak PAS reaction was found in the diazepam treated group of rats. This finding was in agreement with the study conducted by Girgis et al, (2010). Mild expression of P53 was detected in nuclei of some neuronal cells, Bittigau et al (2002) showed that phenobarbital, diazepam and clonazepam caused widespread apoptotic neurodegeneration in the brains of rats.…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Effects of Dependence of Tramadol, Diazepam and Their Combination on the Brain of Albino Rats: Biochemical, Histological and Immunohistochemical Study

Badawy

Hammad

Amin

et al. 2014

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

View full text Add to dashboard Cite

Introduction: Nowadays tramadol is the most common drug of abuse. Egyptian surveys found a gradual increase in the use of tramadol among Egyptians. It has been associated with a wide range of drug abuse such as benzodiazepines. Aim of the work: This study aimed to evaluate the effects of dependence of tramadol, diazepam, and their combination on biochemical, histopathological and immunohistochemical changes of brain of adult albino rats.Material and Methods: Forty adult male albino rats were divided into four equal groups as follows: Group I (control) received1ml normal saline (0.9%NaCl) once orally for one month. Group II (tramadol dependent) received increasing therapeutic doses of tramadol orally for one month, Group III (diazepam dependent) received increasing therapeutic doses of diazepam orally for one month, Group IV (tramadol and diazepam dependent) received increasing therapeutic doses of tramadol and diazepam orally for one month. Blood samples were collected from all groups for evaluation of serum cortisol level. Brain was excised for biochemical, histopathological and immunohistolochemical studies. Results: Compared to the control group, serum cortisol level was significantly decreased in tramadol dependent and combined tramadol and diazepam dependent groups. In all experimental dependent groups, brain cholinesterase level was not changed and the brain showed histopathological and immunohistochemical changes Conclusion and recommendation: Tramadol or diazepam dependence for long time affects the brain cells and the combination of both of them leads to more neurotoxic effect. Therefore it is recommended that tramadol or diazepam should be taken only with the prescription of doctor and self medication of these drugs may be hazardous.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%