Cellular and molecular effects of bleomycin are modulated by heat shock in Saccharomyces cerevisiae

Keszenman, Deborah J.; Candreva, Ema C.; Nunes, Elia

doi:10.1016/s0921-8777(99)00056-7

Cited by 17 publications

(23 citation statements)

References 39 publications

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“…For example, repair of DNA damage measured as unscheduled DNA synthesis was higher in the cells of mice and rats kept under DR compared with cells from ad libitum fed animals [49]. Similarly, pretreatment with heat shock resulted in improved DNA repair in yeast [50]. More generally, sirtuin protein (SIRT6) has recently been shown to stimulate DNA repair in cells under increased oxidative stress [51].…”

Section: Discussionmentioning

confidence: 99%

The effect of sexual harassment on lethal mutation rate in femaleDrosophila melanogaster

et al. 2013

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The rate by which new mutations are introduced into a population may have far-reaching implications for processes at the population level. Theory assumes that all individuals within a population have the same mutation rate, but this assumption may not be true. Compared with individuals in high condition, those in poor condition may have fewer resources available to invest in DNA repair, resulting in elevated mutation rates. Alternatively, environmentally induced stress can result in increased investment in DNA repair at the expense of reproduction. Here, we directly test whether sexual harassment by males, known to reduce female condition, affects female capacity to alleviate DNA damage in Drosophila melanogaster fruitflies. Female gametes can repair double-strand DNA breaks in sperm, which allows manipulating mutation rate independently from female condition. We show that male harassment strongly not only reduces female fecundity, but also reduces the yield of dominant lethal mutations, supporting the hypothesis that stressed organisms invest relatively more in repair mechanisms. We discuss our results in the light of previous research and suggest that social effects such as density and courtship can play an important and underappreciated role in mediating condition-dependent mutation rate.

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Section: Discussionmentioning

confidence: 99%

The effect of sexual harassment on lethal mutation rate in femaleDrosophila melanogaster

et al. 2013

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“…Thereafter, cells were incubated in PBS at 30 • C (liquid holding: LH, 0 ≤ t ≤ 24 h). Either immediately after irradiation (IR) or after different times after incubation in PBS, aliquots of the cell suspensions and respective unirradiated controls were removed for DNA chromosomal analysis and for surviving fraction determinations in YPDA [9,13].…”

Section: Treatmentsmentioning

confidence: 99%

“…Chromosome separation was carried out by transverse alternating field electrophoresis (TAFE; Gene Line II, Beckman, Fullerton, CA). Analysis of chromosomal DNA bands was performed by laser densitometry of the gel photograph negatives (Software: Gel Scan XL Pharmacia) [9,12,13]. Each photograph shows the chromosomal bands corresponding to each cell sample (5 × 10 8 cells/ml).…”

Section: Chromosomal Dna Analysismentioning

confidence: 99%

“…Quantification is possible after laser absorbance analysis [14]. Estimation of mean values of induced DNA double-strand breaks (DBSs) and of corresponding standard deviations were based on the Poisson distribution as: −L Ix/Io, where Ix corresponds to the integral (surface) of a given chromosomal band in the densitograms after treatment and Io is the integral of the same chromosomal band in the respective control sample [9,[13][14][15][16].…”

Section: Chromosomal Dna Analysismentioning

confidence: 99%

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HDF1 and RAD17 Genes are Involved in DNA Double-strand Break Repair in Stationary Phase Saccharomyces cerevisiae

et al. 2008

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DNA repair, checkpoint pathways and protection mechanisms against different types of perturbations are critical factors for the prevention of genomic instability. The aim of the present work was to analyze the roles of RAD17 and HDF1 gene products during the late stationary phase, in haploid and diploid yeast cells upon gamma irradiation. The checkpoint protein, Rad17, is a component of a PCNA-like complex-the Rad17/Mec3/Ddc1 clamp-acting as a damage sensor; this protein is also involved in double-strand break (DBS) repair in cycling cells. The HDF1 gene product is a key component of the non-homologous end-joining pathway (NHEJ). Diploid and haploid rad17 /rad17 , and hdf1 Saccharomyces cerevisiae mutant strains and corresponding isogenic wild types were used in the present study. Yeast cells were grown in standard liquid nutrient medium, and maintained at 30 • C for 21 days in the stationary phase, without added nutrients. Cell samples were irradiated with 60 Co γ rays at 5 Gy/s, 50 Gy ≤ Dabs ≤ 200 Gy. Thereafter, cells were incubated in PBS (liquid holding: LH, 0 ≤ t ≤ 24 h). DNA chromosomal analysis (by pulsed-field electrophoresis), and surviving fractions were determined as a function of absorbed doses, either immediately after irradiation or after LH. Our results demonstrated that the proteins Rad17, as well as Hdf1, play essential roles in DBS repair and survival after gamma irradiation in the late stationary phase and upon nutrient stress (LH after irradiation). In haploid cells, the main pathway is NHEJ. In the diploid state, the induction of LH recovery requires the function of Rad17. Results are compatible with the action of a network of DBS repair pathways expressed upon different ploidies, and different magnitudes of DNA damage.

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“…The overlap between different stress responses contributes at least in part to the phenomenon of cross-protection [Mager and De Kruijff, 1995;Hohmann and Mager, 1997]. As a result of this phenomenon, HS in yeast results in resistance to the lethality and mutagenicity of different DNA-damaging agents [Mitchel and Morrison, 1982;Nunes et al, 1993;Sanchez et al, 1993;Keszenman et al, 1994;Keszenman et al, 2000].…”

Section: Introductionmentioning

confidence: 99%

RAD6gene is involved in heat shock induction of bleomycin resistance inSaccharomyces cerevisiae

Keszenman¹,

Candreva²,

Sánchez³

et al. 2004

Environ. Mol. Mutagen.

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Cells react to environmental and endogenous challenges such as high temperature, reactive oxygen species, DNA damage, and nutrient starvation by activating several defense mechanisms known as stress responses. An important feature is the overlap between different stress responses that contributes at least in part to the phenomenon of cross-protection. We previously demonstrated that pretreatment with a heat shock (HS) induces resistance to the lethal and mutagenic effects of the antineoplastic drug Bleomycin (BLM) in wild-type Saccharomyces cerevisiae. At the DNA level, the HS resulted in more efficient repair of BLM-induced DNA damage. In the present study, we have investigated the mechanisms involved in this HS-induced BLM resistance. Since the RAD6 gene is involved in the ubiquitin system and DNA repair, we analyzed the effects of HS on the lethality of BLM in a rad6Delta (ubc2) mutant strain of S. cerevisiae. The rad6Delta mutant was more sensitive to the lethal effects of BLM than wild-type yeast and HS had no effect on the lethality of BLM in the mutant. Analysis of cell proliferation kinetics indicated that the HS-induced cell cycle delay observed in the wild-type yeast was absent in the rad6Delta mutant strain. BLM treatment impaired mutant cell proliferation, and HS had no effect on the delayed cell kinetics of the mutant. In addition, pulsed-field electrophoresis of chromosomes damaged by BLM indicated that there was very little recovery from damage in the mutant after 24 hr of incubation in BLM-free nutrient medium, and that HS had little effect on the recovery. These data indicate that the RAD6 gene is involved in the HS-induced BLM resistance observed in the isogenic wild-type strain.

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Cellular and molecular effects of bleomycin are modulated by heat shock in Saccharomyces cerevisiae

Cited by 17 publications

References 39 publications

The effect of sexual harassment on lethal mutation rate in femaleDrosophila melanogaster

The effect of sexual harassment on lethal mutation rate in femaleDrosophila melanogaster

HDF1 and RAD17 Genes are Involved in DNA Double-strand Break Repair in Stationary Phase Saccharomyces cerevisiae

RAD6gene is involved in heat shock induction of bleomycin resistance inSaccharomyces cerevisiae

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