Cellular distribution of bovine leukemia virus proteins gp51SU, Pr72env, and Pr66gag-pro in persistently infected cells

Llames, L.; Goyache, J.; Doménech, Ana; Montaña, Ana Vicente; Suárez, G.; Gómez-Lucı́a, Esperanza

doi:10.1016/s0168-1702(01)00291-x

Cited by 9 publications

(10 citation statements)

References 21 publications

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“…This post-translational maturation is carried out by the viral protease p14 which is encoded by a region located between the gag and the pol genes. p14 is synthesized from a gag-protease precursor (pr66 gag-prt ) via a frameshift suppression of the gag termination codon by a lysine-specific tRNA [116]. The pr66 gag-prt precursor localizes at the surface of polarized cells [117].…”

Section: The Blv Genomementioning

confidence: 99%

Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human

et al. 2007

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In 1871, the observation of yellowish nodules in the enlarged spleen of a cow was considered to be the first reported case of bovine leukemia. The etiological agent of this lymphoproliferative disease, bovine leukemia virus (BLV), belongs to the deltaretrovirus genus which also includes the related human T-lymphotropic virus type 1 (HTLV-1). This review summarizes current knowledge of this viral system, which is important as a model for leukemogenesis. Recently, the BLV model has also cast light onto novel prospects for therapies of HTLV induced diseases, for which no satisfactory treatment exists so far.

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Section: The Blv Genomementioning

confidence: 99%

Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human

et al. 2007

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“…At the herd level, relative resistance to BLV-dependent B-cell proliferation and lymphocytosis among seropositive cows was associated with bovine lymphocyte antigen (BoLA)-DA7, whereas susceptibility was associated with BoLA-DA12.3 (36). These associations were also confirmed at the family level, where BoLA phenotypes were used as haplotypic markers (20,37).…”

Section: Discussionmentioning

confidence: 80%

“…Very interesting results were obtained by Llames et al (20). With the use of monoclonal antibodies and immunofluorescence assay, they investigated cellular distribution of BLV proteins: gp51SU, Pr72env, and Pr66(gag-pro), in live, persistently infected FLK-BLV cells.…”

Section: Discussionmentioning

confidence: 99%

“…Although the development of a tumoural form of bovine leukosis has been described as dependent on host susceptibility (20,26,37), currently, there is no evidence whether some genotypes can influence or induce these neoplasias. At the herd level, relative resistance to BLV-dependent B-cell proliferation and lymphocytosis among seropositive cows was associated with bovine lymphocyte antigen (BoLA)-DA7, whereas susceptibility was associated with BoLA-DA12.3 (36).…”

Section: Discussionmentioning

confidence: 99%

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Flow Cytometric Determination of the Expression of gp 51 Protein of Bovine Leukaemia Virus in Experimentally Infected Sheep

Szczotka

2014

Bulletin of the Veterinary Institute in Pulawy

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The study was performed on lambs experimentally infected with bovine leukaemia virus (BLV). The presence of BLV antibodies in sera of infected animals was detected by agar gel immunodiffusion test and ELISA. Proviral DNA was detected by PCR and nested PCR. Dual-colour flow cytometry analysis was performed with the use of specific monoclonal antibodies against lymphocyte CD markers and gp51 viral envelope protein, followed by incubation with fluorescent-labelled secondary antibodies conjugated with FITC or PE. Gp51 viral envelope protein was detected in tumours caused by BLV infection. The BLV infection resulted in depletion of CD4+ lymphocytes, increase in CD8+ lymphocytes, and decrease in CD4+ to CD8+ ratio in infected sheep. Proliferation of IgM+ CD19+ cells was also detected. These cells had an immature character without tendency to differentiate, and their vitality was prolonged. Flow cytometry enabled detection of gp51 expression in sheep blood lymphocytes at the early stages of the infection, before detection of serum antibodies using ELISA.

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“…Although some researchers suggest viral genomes are replicated and distributed evenly between daughter cells in mitosis [17,18], recent reports have indicated in the dividing cells, virus proteins are likely to be unevenly distributed but concentrated in only one daughter cell [19,20]. Vesicular stomatitis virus (VSV) can infect cattle, horses and pigs, leading to severe oral diseases.…”

Section: Infection Heterogeneitymentioning

confidence: 99%

Use of cell morphology as an early bio-sensor for viral infection

Liu

Matsuo

et al. 2013

The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems

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This paper reports a correlation between cellular morphology and the ability of adapting Vesicular stomatitis virus (VSV) infection. A time-lapse approach was employed to track the individual difference between homologous cells in adopting viral infection. Our single-cell analysis indicates that upon viral infection, mature cells that are in spindle shape are less likely to be infected after 24 hour infection. On the other hand, cells undergoing proliferation, which are in rounder shape, tend to adopt much higher viral infection within the same amount of time. This fact suggests cellular morphology may to be an early bio-sensor for viral infection. The findings in this paper could potentially be applied to other viral infection models.

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Cellular distribution of bovine leukemia virus proteins gp51SU, Pr72env, and Pr66gag-pro in persistently infected cells

Cited by 9 publications

References 21 publications

Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human

Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human

Flow Cytometric Determination of the Expression of gp 51 Protein of Bovine Leukaemia Virus in Experimentally Infected Sheep

Use of cell morphology as an early bio-sensor for viral infection

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