Cellular responses induced in vitro by iron (Fe) in a central nervous system cell line (U343MGa)

Alcântara, Diego Di Felipe Ávila; Ribeiro, Helem Ferreira; Matos, Leomá Albuquerque; Sousa, J.M.C.; Burbano, Rommel Rodrı́guez; Bahia, Marcelo de Oliveira

doi:10.4238/2013.may.13.9

Cited by 6 publications

(7 citation statements)

References 27 publications

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“…These results are in line with literature reports showing no or much slower effects for Fe 2+ only and H 2 O 2 only. Regarding FeSO 4 induced toxicity at the present concentration, Alcântara et al have shown that there is no cytotoxic effect even after 24 h of treatment with the same concentration to a central nervous system cell line . However, Liu et al have shown significant cell death in hippocampal slice culture when treated with 0.2 mM FeSO 4 for 24 h .…”

Section: Resultsmentioning

confidence: 70%

Ascorbate Protects Neurons against Oxidative Stress: A Raman Microspectroscopic Study

Dutta¹,

Gautam²,

Chatterjee³

et al. 2015

ACS Chem. Neurosci.

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Oxidative stress due to excessive accumulation of reactive oxygen or nitrogen species in the brain as seen in certain neurodegenerative diseases can have deleterious effects on neurons. Hydrogen peroxide, endogenously generated in neurons under normal physiological conditions, can produce an excess of hydroxyl radical via a Fenton mediated mechanism. This may induce acute oxidative injury if not scavenged or removed effectively by antioxidants. There are several biochemical assay methods to estimate oxidative injury in cells; however, they do not provide information on the biochemical changes as the cells get damaged progressively under oxidative stress. Raman microspectroscopy offers the possibility of real time monitoring of the chemical composition of live cells undergoing oxidative stress under physiological conditions. In the present study, a hippocampal neuron coculture was used to observe the acute impact of hydroxyl radicals generated by hydrogen peroxide in the presence of Fe(2+) (Fenton reaction). Raman peaks related to nucleic acids (725, 782, 1092, 1320, 1340, 1420, and 1576 cm(-1)) showed time-dependent changes over the experimental period (60 min), indicating the breakdown of the phosphodiester backbone as well as nuclear bases. Interestingly, ascorbic acid (a potent antioxidant) when cotreated with Fenton reactants showed protection of cells as inferred from the Raman spectra, presumably by scavenging hydroxyl radicals. Little or no change in the Raman spectra was observed for untreated control cells and for cells exposed to Fe(2+) only, H2O2 only, and ascorbate only. A live-dead assay study also supported the current observations. Hence, Raman microspectroscopy has the potential to be an excellent noninvasive tool for early detection of oxidative stress that is seen in neurodegenerative diseases.

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Section: Resultsmentioning

confidence: 70%

Ascorbate Protects Neurons against Oxidative Stress: A Raman Microspectroscopic Study

Dutta¹,

Gautam²,

Chatterjee³

et al. 2015

ACS Chem. Neurosci.

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“…Iron sulphate reduces cell viability as assessed by MTT assay in the human glioblastoma astrocytic cell line U343 MG-a (1). Concurring results were observed by He et al .…”

Section: Discussionmentioning

confidence: 99%

“…ROS may also react with DNA, causing its damage. There are many reports about iron-induced genotoxicity and mutagenicity, among which one is positing that iron compounds can induce mutations in Salmonella Typhimurium (1).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of iron (III), molybdenum (III), and their mixtures in BALB/3T3 and HepG2 cells

Terpiłowska

Siwicka-Gieroba

Siwicki

2018

Journal of Veterinary Research

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IntroductionIron and molybdenum are essential trace elements for cell metabolism. They are involved in maintaining proper functions of enzymes, cell proliferation, and metabolism of DNA.Material and MethodsBALB/3T3 and HepG2 cells were incubated with iron chloride or molybdenum trioxide at concentrations from 100 to 1,400 μM. The cells were also incubated in mixtures of iron chloride at 200 μM plus molybdenum trioxide at 1,000 μM or iron chloride at 1,000 μM plus molybdenum trioxide at 200 μM. Cell viability was determined with MTT reduction, LHD release, and NRU tests.ResultsA decrease in cell viability was observed after incubating both cell lines with iron chloride or molybdenum trioxide. In cells incubated with mixtures of these trace elements, a decrease in cell viability was observed, assessed by all the used assays.ConclusionIron (III) and molybdenum (III) decrease cell viability in normal and cancer cells. A synergistic effect of the mixture of these elements was observed.

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“…There are many reports of iron genotoxicity. Iron compounds induce DNA damage, mutations in Salmonella Typhimurium, and formation of micronuclei (2).…”

Section: Introductionmentioning

confidence: 99%

Cell viability in normal fibroblasts and liver cancer cells after treatment with iron (III), nickel (II), and their mixture

Terpiłowska

Siwicka-Gieroba

Siwicki

2018

Journal of Veterinary Research

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IntroductionNickel and iron are very commonly occurring metals. Nickel is used in industry, but nowadays it is also used in medical biomaterials. Iron is an element necessary for cell metabolism and is used in diet supplements and biomaterials, whence it may be released along with nickel.Material and MethodsBALB/3T3 and HepG2 cells were incubated with iron chloride or nickel chloride at concentrations ranging from 100 to 1,400 μM. The following mixtures were used: iron chloride 200 μM plus nickel chloride 1,000 μM, or iron chloride 1,000 μM plus nickel chloride 200 μM. The cell viability was determined with MTT, LHD, and NRU tests.ResultsA decrease in cell viability was observed after incubating the BALB/3T3 and HepG2 cells with iron chloride or nickel chloride. A synergistic effect was observed after iron chloride 1,000 μM plus nickel chloride 200 μM treatment in all assays. Moreover, the same effect was observed in the pair iron chloride 200 μM plus nickel chloride 1,000 μM in the LDH and NRU assays.ConclusionsIron (III) and nickel (II) decrease cell viability. Iron chloride at a concentration of 200 μM protects mitochondria from nickel chloride toxicity.

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Cellular responses induced in vitro by iron (Fe) in a central nervous system cell line (U343MGa)

Cited by 6 publications

References 27 publications

Ascorbate Protects Neurons against Oxidative Stress: A Raman Microspectroscopic Study

Ascorbate Protects Neurons against Oxidative Stress: A Raman Microspectroscopic Study

Cytotoxicity of iron (III), molybdenum (III), and their mixtures in BALB/3T3 and HepG2 cells

Cell viability in normal fibroblasts and liver cancer cells after treatment with iron (III), nickel (II), and their mixture

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