Cellular responses of embryonic hyaline cartilage to experimental wounding <i>in vitro</i>

Walker, Elizabeth A.; Verner, A.; Flannery, Carl R.; Archer, Charles W.

doi:10.1002/jor.1100180105

Cited by 28 publications

(8 citation statements)

References 40 publications

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“…However, studies demonstrated that suture can irreversibly damage the cartilage. Suturing the membrane generates partial-thickness lesions of the articular cartilage which may not heal and enlarge with time [ 7 , 8 ]. Hunzinker et al [ 9 ], to establish the potential damage of sutures in cartilage, sutured the surrounding articular cartilage of large, partial-thickness trochlear defects in 18 adult goats.…”

Section: Discussionmentioning

confidence: 99%

“…Initially, the membrane was sutured to the defect to ensure implant stability. However, suture generates partial-thickness lesions of the articular cartilage which may not heal and enlarge with time, leading to persisting symptoms and premature degeneration [ 7 – 9 ]. To avoid membrane suture, fibrin glue has been introduced, although some studies secured the membrane with suture [ 10 , 11 ], fibrin glue [ 12 – 21 ], or both [ 22 – 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Fibrin glue does not assist migration and proliferation of chondrocytes in collagenic membranes: an in vitro study

et al. 2022

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Background Some authors secured the membrane during matrix-induced autologous chondrocyte implantation (mACI) with fibrin glue or did not use a formal fixation. The real impact of fibrin glue addition on chondrocytes migration and proliferation has not yet been clarified. This study evaluated the impact of fibrin glue on a chondrocyte loaded collagenic membrane. Methods A resorbable collagen I/III porcine derived membrane commonly employed in AMIC was used for all experiments. Chondrocytes from three difference donors were used. At 1-, 2-, 3-, 4-, 6-, and at 8-week the membranes were embedded in Mounting Medium with Dapi (ABCAM, Cambridge, UK). The Dapi contained in the mounting medium ties the DNA of the cell nucleus and emits a blue fluorescence. In this way, the spreading of the cells in the membrane can be easily monitored. The outcomes of interest were to evaluate (1) cell migration and (2) cell proliferation within the porous membrane layer. DAPI/nuclei signals were analysed with fluorescence microscope under a magnification of 100-fold. Results The no-fibrin group demonstrated greater migration of the cells within the membrane. Although migration resulted higher in the no-fibrin group at every follow-up, this difference was significant only at week 1 (P < 0.001), 2 (P = 0.004), and 3 (P = 0.03). No difference was found at week 3, 6, and 8. The no-fibrin group demonstrated greater proliferation of the chondrocytes within the membrane. These differences were significant at week 4 (P < 0.0001), 6 (P < 0.0001), 8 (P < 0.0001). Conclusion The use of fibrin glue over a resorbable membrane leads to lower in vitro proliferation and migration of chondrocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fibrin glue does not assist migration and proliferation of chondrocytes in collagenic membranes: an in vitro study

et al. 2022

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“…In previous studies, it was suggested that chondrocyte necrosis occurred because of mechanical forces, 23 , 26 , 32 , 33 loss of a survival factor, or influx of an inhibitory substance. 23 , 34 The current observed chondrocyte necrosis was morphologically compatible with coagulative necrosis, which is often associated with ischemia. 35 The ischemic chondronecrosis that is observed in equine articular OC is centered on necrotic vessels within zones of the epiphyseal growth cartilage that are not accessible by diffusion, typically comprises more than 250 cells and induces vigorous proliferation of vessels in adjacent, viable growth cartilage.…”

Section: Discussionmentioning

confidence: 53%

Local Morphological Response of the Distal Femoral Articular–Epiphyseal Cartilage Complex of Young Foals to Surgical Stab Incision and Potential Relevance to Cartilage Injury and Repair in Children

et al. 2013

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Objective: Describe the local morphological response of the articular–epiphyseal cartilage complex to surgical stab incision in the distal femur of foals, with emphasis on the relationship between growth cartilage injury, enchondral ossification, and repair. Design: Nine foals were induced into general anesthesia at the age of 13 to 15 days. Four full-thickness stab incision defects were created in the cartilage on the lateral aspect of the lateral trochlear ridge of the left distal femur. Follow-up examination was carried out from 1 to 49 days postoperatively, including examination of intact bones, sawed slabs, and histological sections. Results: Incision defects filled with cells displaying fibroblast-, chondrocyte-, and osteoblast-like characteristics, potentially validating the rationale behind the drilling of stable juvenile osteochondritis dissecans lesions in children. Incisions induced necrosis within the cartilage on the margins at all depths of the defects. Sharp dissection may therefore be contraindicated in cartilage repair in young individuals. Incisions caused a focal delay in enchondral ossification in 2 foals, apparently related to the orientation of the incision defect relative to the direction of ossification. Defects became progressively surrounded by subchondral bone, in which granulation tissue containing clasts and foci of osteoblast-like cells was observed. Continued enchondral ossification was therefore likely to result in healing of uncomplicated defects to morphologically normal bone. Conclusions: Epiphyseal growth cartilage injury had the potential to exert a negative effect on enchondral ossification. Enchondral ossification exerted a beneficial effect on repair. This relationship warrants consideration in future studies of cartilage injury and repair within the articular–epiphyseal cartilage complex of all species.

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“…Both authors reported better outcomes in the glued AMIC group. Membrane sutures produces fissures in the articular cartilage which may not heal, and may enlarge with time 75 , 76 . Suturing induces local cartilage impairment which may lead to pain, reduced healing, and premature osteoarthritis 77 .…”

Section: Discussionmentioning

confidence: 99%

Autologous matrix-induced chondrogenesis is effective for focal chondral defects of the knee

Migliorini

Maffulli

Baroncini

et al. 2022

Sci Rep

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Focal chondral defects of the knee are common and their management is challenging. This study investigated the efficacy and safety of Autologous Matrix-Induced Chondrogenesis (AMIC) for focal chondral defects of the knee. A systematic review and meta-analysis was conducted (according to the 2020 PRISMA statement) to investigate the efficacy of AMIC in improving symptoms and to compare AMIC versus microfracture (MFx). In January 2022, the following databases were accessed: Pubmed, Web of Science, Google Scholar, Embase. No time constrain was used for the search. All the clinical trials investigating AMIC and/or those comparing AMIC versus MFx for focal chondral defects of the knee were accessed. Only studies published in peer reviewed journals were considered. Studies which investigated other locations of the defects rather than knee were not eligible, nor those reporting data form mixed locations. Studies which reported data on revision settings, as well as those investigating efficacy on kissing lesions or multiple locations, were not suitable. The mean difference (MD) and odd ratio (OR) effect measure were used for continuous and binary data, respectively. Data from 18 studies (548 patients) were retrieved with a mean follow-up of 39.9 ± 26.5 months. The mean defect size was 3.2 ± 1.0 cm2. The visual analogue scale (VAS) decreased of − 3.9/10 (95% confidence interval (CI) − 4.0874 to -3.7126), the Tegner Activity Scale increased of + 0.8/10 (95% CI 0.6595 to 0.9405). The Lysholm Knee Scoring System increased of + 28.9/100 (95% CI 26.8716 to 29.1284), as did the International Knee Documentation Committee (IKDC) + 33.6/100 (95% CI 32.5800 to 34.6200). At last follow-up no patient showed signs of hypertrophy. 4.3% (9 of 210) of patients underwent revision procedures. The rate of failure was 3.8% (9 of 236). Compared to MFx, AMIC demonstrated lower VAS score (MD: − 1.01; 95% CI − 1.97 to 0.05), greater IKDC (MD: 11.80; 95% CI 6.65 to 16.94), and lower rate of revision (OR: 0.16; 95% CI 0.06 to 0.44). AMIC is effective for focal chondral defects of the knee. Furthermore, AMIC evidenced greater IKDC, along with a lower value of VAS and rate of revision compared to MFx.

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Cellular responses of embryonic hyaline cartilage to experimental wounding in vitro

Cited by 28 publications

References 40 publications

Fibrin glue does not assist migration and proliferation of chondrocytes in collagenic membranes: an in vitro study

Fibrin glue does not assist migration and proliferation of chondrocytes in collagenic membranes: an in vitro study

Local Morphological Response of the Distal Femoral Articular–Epiphyseal Cartilage Complex of Young Foals to Surgical Stab Incision and Potential Relevance to Cartilage Injury and Repair in Children

Autologous matrix-induced chondrogenesis is effective for focal chondral defects of the knee

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