Cellular senescence induced loss of stem cell proportion in the skin in vitro

Isolation of human epidermal stem cells by adherence and the reconstruction of skin equivalents

“…The expression patterns of p63, which is currently regarded as a stem cell marker, were found to be similar to those of PCNA [5,6,25]. Moreover, we recently suggested that p63 may have a role in maintaining the proliferative potential of keratinocytes [26]. As shown by our Western blot analysis, p63 is more strongly expressed by RA cells than by SA or NA cells.…”

Terrein, a fungal metabolite, inhibits the epidermal proliferation of skin equivalents

“…However, the expression of PCNA was dramatically reduced in terrein-treated SEs. p63 plays an important role in the development of epithelium in several organs including skin [7], and recently, we suggested that p63 participates in the maintenance of keratinocyte proliferative potential [8]. As shown in Fig.…”

Isolation of small-sized human epidermal progenitor/stem cells by Gravity Assisted Cell Sorting (GACS)

“…Furthermore, FACS analysis demonstrated that nontrapped cells had a larger a6 integrin bri CD71 dim cell population than trapped cells. Although, in culture conditions, true epidermal stem cells would alter their phenotypes due to out of a niche condition [20], Youn et al [21] considered a6 integrin bri CD71 dim cell population as candidate keratinocytes stem cells in culture conditions. Thus, our results also implied the ability of GACS to enrich epidermal keratinocytes progenitor/stem cell subpopulation, more efficiently for cultured cells than primary cells freshly removed from the tissue of original, in this case skin.…”