Cellular Uptake and Cytotoxicity of Silica Nanotubes

Nan, Anjan; Bai, Xia; Son, Sang Jun; Lee, Sang Bok; Ghandehari, Hamidreza

doi:10.1021/nl0802741

Cited by 198 publications

(149 citation statements)

References 28 publications

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“…However, in most situations, the positively charged gene carrier suffers from cytotoxicity. 20 In our study, the 3-aminopropyltriethoxysilane-modified nanosheets and pristine nanosheets had similar transfection efficiencies and cell survival rates. This finding suggests that the positive charge on silica nanosheets and the electronic interaction between nanosheets and seeded cells may not be the dominating mechanisms behind the enhanced gene delivery observed in this study.…”

Section: Resultssupporting

confidence: 56%

Nanosheet transfection: effective transfer of naked DNA on silica glass

Huang

Ariga

et al. 2015

NPG Asia Mater

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Existing gene delivery technologies using nonviral vectors or physical stimulation are limited by cytotoxicity. Here, we reveal an easy and new method to fabricate silica upright nanosheets, which can be easily scaled up and used for gene delivery. We demonstrate that naked DNA can be transferred into difficult-to-transfect cells (for example, stem cells) by plating cells on silica glass with the upright nanosheets without using any vector. Gene entry is probably achieved through the integrin-FAK-Rho signaling axis, which can be activated by the cytoskeleton rearrangement on nanosheets in a limited time frame ('transfection window'). Transfecting naked GATA4-binding protein 4 plasmids into stem cells can upregulate the other two important cardiac marker genes myocyte enhancer factor 2C and T-box 5. The transfected mesenchymal stem cells express the cardiac marker proteins at 7 days after transfection, which confirms the success of the innovative gene delivery approach. The vector-free silica nanosheet-induced transfection is simple and effective but faster and safer than the conventional technologies.

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Section: Resultssupporting

confidence: 56%

Nanosheet transfection: effective transfer of naked DNA on silica glass

Huang

Ariga

et al. 2015

NPG Asia Mater

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“…Some previous data indicated a direct link between cytotoxicity and particle-cell association. 43,44 However, our results revealed that np20 showed higher uptake, while more significant inhibitory effects on the ECs function were detected upon incubation with np80 ( Figures 5B and 7), implying cellular uptake was not the only determinant of biological outcome; other factors, such as shape, may be involved in the interaction. Indeed, elegant experiments have demonstrated the significant role of the shape of NPs in the bioactivity.…”

Section: Discussionmentioning

confidence: 70%

Interaction of hydroxyapatite nanoparticles with endothelial cells: internalization and inhibition of angiogenesis in vitro through the PI3K/Akt pathway

Shi¹,

Zhou²,

Huang³

et al. 2017

IJN

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Nano-hydroxyapatite (nano-HAP) has been proposed as a better candidate for bone tissue engineering; however, the interactions of nano-HAP with endothelial cells are currently unclear. In this study, HAP nanoparticles (HANPs; 20 nm np20 and 80 nm np80) and micro-sized HAP particles (m-HAP; 12 μm) were employed to explore and characterize cellular internalization, subcellular distribution, effects of HANPs on endothelial cell function and underlying mechanisms using human umbilical vein endothelial cells (HUVECs) as an in vitro model. It was found that HANPs were able to accumulate in the cytoplasm, and both adhesion and uptake of the HANPs followed a function of time; compared to np80, more np20 had been uptaken at the end of the observation period. HANPs were mainly uptaken via clathrin- and caveolin-mediated endocytosis, while macropinocytosis was the main pathway for m-HAP uptake. Unexpectedly, exposure to HANPs suppressed the angiogenic ability of HUVECs in terms of cell viability, cell cycle, apoptosis response, migration and capillary-like tube formation. Strikingly, HANPs reduced the synthesis of nitric oxide (NO) in HUVECs, which was associated with the inhibition of phosphatidylinositol 3-kinase (PI3K) and phosphorylation of eNOS. These findings provide additional insights into specific biological responses as HANPs interface with endothelial cells.

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“…Thirdly, they have open ends that make the inner surface accessible and allow subsequent incorporation of species within the tubes and can be used as a gate to control drug release. [11,12] In addition, for photoacoustic imaging and photothermal therapy, the hollow core can lower the heat capacity to allow better pulse heating. [13] Compared to their spherical counterparts, the elongated nanostructures have longer blood circulation times [14] and show multi-valence effect, that is, multiple binding sites of a functionalized NT to one cell, leading to improved cell adhesion and more effective targeting.…”

Section: Introductionmentioning

confidence: 99%