Cellulose‐based porous scaffold for bone tissue engineering applications: Assessment of h<scp>MSC</scp> proliferation and differentiation

Demitri, Christian; Raucci, Maria Grazia; Giuri, Antonella; Benedictis, Vincenzo Maria De; Giugliano, Daniela; Calcagnile, Paola; Sannino, Alessandro; Ambrosio, Luigi

doi:10.1002/jbm.a.35611

Cited by 34 publications

(15 citation statements)

References 22 publications

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“…Furthermore, in vitro biological study was performed to evaluate the effect of surface properties on cell proliferation [ 64 ]. In particular, hMSCs proliferation was quantified after one, three, and seven days of culture on the CS/HA coatings, and the results are shown in Figure 6 .…”

Section: Resultsmentioning

confidence: 99%

In-Situ Synthesis and Characterization of Chitosan/Hydroxyapatite Nanocomposite Coatings to Improve the Bioactive Properties of Ti6Al4V Substrates

et al. 2020

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Ti6Al4V alloy is still attracting great interest because of its application as an implant material for hard tissue repair. This research aims to produce and investigate in-situ chitosan/hydroxyapatite (CS/HA) nanocomposite coatings based on different amounts of HA (10, 50 and 60 wt.%) on alkali-treated Ti6Al4V substrate through the sol-gel process to enhance in vitro bioactivity. The influence of different contents of HA on the morphology, contact angle, roughness, adhesion strength, and in vitro bioactivity of the CS/HA coatings was studied. Results confirmed that, with increasing the HA content, the surface morphology of crack-free CS/HA coatings changed for nucleation modification and HA nanocrystals growth, and consequently, the surface roughness of the coatings increased. Furthermore, the bioactivity of the CS/HA nanocomposite coatings enhanced bone-like apatite layer formation on the material surface with increasing HA content. Moreover, CS/HA nanocomposite coatings were biocompatible and, in particular, CS/10 wt.% HA composition significantly promoted human mesenchymal stem cells (hMSCs) proliferation. In particular, these results demonstrate that the treatment strategy used during the bioprocess was able to improve in vitro properties enough to meet the clinical performance. Indeed, it is predicted that the dense and crack-free CS/HA nanocomposite coatings suggest good potential application as dental implants.

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Section: Resultsmentioning

confidence: 99%

In-Situ Synthesis and Characterization of Chitosan/Hydroxyapatite Nanocomposite Coatings to Improve the Bioactive Properties of Ti6Al4V Substrates

et al. 2020

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“…Several materials have been developed and analyzed to be used for this purpose, including bioactive ceramics such as hydroxyapatite (HA) [5], beta-tricalcium phosphate (b-TCP) [6], biphasic calcium phosphate (BCP) [7], calcium phosphate 2 International Journal of Polymer Science cements [8], bioactive glass [9], and several biodegradable polymers [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features

Dimida

Barca

Cancelli

et al. 2017

International Journal of Polymer Science

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Genipin (GN) is a natural molecule extracted from the fruit of Gardenia jasminoides Ellis according to modern microbiological processes. Genipin is considered as a favorable cross-linking agent due to its low cytotoxicity compared to widely used cross-linkers; it cross-links compounds with primary amine groups such as proteins, collagen, and chitosan. Chitosan is a biocompatible polymer that is currently studied in bone tissue engineering for its capacity to promote growth and mineral-rich matrix deposition by osteoblasts in culture. In this work, two genipin cross-linked chitosan scaffolds for bone repair and regeneration were prepared with different GN concentrations, and their chemical, physical, and biological properties were explored. Scanning electron microscopy and mechanical tests revealed that nonremarkable changes in morphology, porosity, and mechanical strength of scaffolds are induced by increasing the cross-linking degree. Also, the degradation rate was shown to decrease while increasing the crosslinking degree, with the high cross-linking density of the scaffold disabling the hydrolysis activity. Finally, basic biocompatibility was investigated in vitro, by evaluating proliferation of two human-derived cell lines, namely, the MG63 (human immortalized osteosarcoma) and the hMSCs (human mesenchymal stem cells), as suitable cell models for bone tissue engineering applications of biomaterials.

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“…Indeed, in addition to a very quick swelling/deswelling response, cryogels may also show high elasticity and shape memory, which can make them even injectable for specific biomedical applications [124,125]. So far several cellulose-based porous hydrogels, including different types of cryogels, have been proposed in the literature as smart materials for a wide range of applications, e.g., absorbents in the agriculture field [126], matrices for controlled drug release [127], stomach bulking agents [18] and scaffolds for tissue engineering [128].…”

Section: Micro-and Macroscale: Hydrogel Porositymentioning

confidence: 99%

Investigating the Structure-Related Properties of Cellulose-Based Superabsorbent Hydrogels

Demitri¹,

Madaghiele²,

Raucci³

et al. 2019

Hydrogels - Smart Materials for Biomedical Applications

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Superabsorbent hydrogels are macromolecular networks able to absorb and retain large amounts of water solutions within their fine mesh-like structure. More importantly, they are capable of multiple swelling/shrinking transitions in response to specific environmental cues (e.g., pH, ionic strength, temperature, presence of given electrolytes), thus exhibiting a stimuli-sensitive behavior, which makes them appealing for the design of smart devices in a number of technological fields. In particular, in the last two decades, cellulose-based superabsorbent hydrogels have proven to be an environmentally friendly and cost-effective alternative to acrylamide-based products. This chapter reviews the relationship between the molecular structure of cellulose-based hydrogels and their physicochemical properties. First, the network formation through the use of different cellulose derivatives and chemical or physical crosslinking agents is presented. Successively, the smart swelling capability of the hydrogels as a function of composition and structure is thoroughly discussed. Finally, several approaches to the hydrogel characterization are reviewed, with focus on the assessment of key mechanical, thermal and morphological properties.

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Cellulose‐based porous scaffold for bone tissue engineering applications: Assessment of hMSC proliferation and differentiation

Cited by 34 publications

References 22 publications

In-Situ Synthesis and Characterization of Chitosan/Hydroxyapatite Nanocomposite Coatings to Improve the Bioactive Properties of Ti6Al4V Substrates

In-Situ Synthesis and Characterization of Chitosan/Hydroxyapatite Nanocomposite Coatings to Improve the Bioactive Properties of Ti6Al4V Substrates

Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features

Investigating the Structure-Related Properties of Cellulose-Based Superabsorbent Hydrogels

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