Cellulose Nanocrystals—Bioactive Glass Hybrid Coating as Bone Substitutes by Electrophoretic Co-deposition: In Situ Control of Mineralization of Bioactive Glass and Enhancement of Osteoblastic Performance

Chen, Qiang; Garcia, Rosalina Pérez; Muñoz, Josemari; Larraya, Uxua Pérez de; Garmendia, Nere; Yao, Qingqing; Boccaccını, Aldo R.

doi:10.1021/acsami.5b07294

Cited by 69 publications

(47 citation statements)

References 49 publications

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“…For instance, fibrous cellulose nanocrystals interconnected with bioactive glass are used to cover stainless steel. In vitro studies confirmed cell activity acceleration in terms of attachment, differentiation and mineralization [123]. On the other hand, the same cellulose surface has been enriched with BMP-2-coating to further increase the cell activity.…”

Section: Natural Polymersmentioning

confidence: 77%

Natural and Synthetic Polymers for Bone Scaffolds Optimization

et al. 2020

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Bone tissue is the structural component of the body, which allows locomotion, protects vital internal organs, and provides the maintenance of mineral homeostasis. Several bone-related pathologies generate critical-size bone defects that our organism is not able to heal spontaneously and require a therapeutic action. Conventional therapies span from pharmacological to interventional methodologies, all of them characterized by several drawbacks. To circumvent these effects, tissue engineering and regenerative medicine are innovative and promising approaches that exploit the capability of bone progenitors, especially mesenchymal stem cells, to differentiate into functional bone cells. So far, several materials have been tested in order to guarantee the specific requirements for bone tissue regeneration, ranging from the material biocompatibility to the ideal 3D bone-like architectural structure. In this review, we analyse the state-of-the-art of the most widespread polymeric scaffold materials and their application in in vitro and in vivo models, in order to evaluate their usability in the field of bone tissue engineering. Here, we will present several adopted strategies in scaffold production, from the different combination of materials, to chemical factor inclusion, embedding of cells, and manufacturing technology improvement.

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Section: Natural Polymersmentioning

confidence: 77%

Natural and Synthetic Polymers for Bone Scaffolds Optimization

et al. 2020

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“…in combination with bioglass), have been shown to be suitable as orthopaedic materials. Such CNC-based hybrid materials can accelerate osteoblast attachment, increase their spreading, proliferation, even promote differentiation of mesenchymal cells towards the osteoblast like phenotype (Gorgieva et al 2017), as well as influence mineralization of the native osteoblast extracellular matrix positively (Chen et al 2015). Nevertheless, neither of these studies reports or even considers the internalization of materials (or their parts) by osteoblasts.…”

Section: Characterization Of Differently Modified Cncsmentioning

confidence: 99%

Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells

et al. 2017

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Covalent conjugation of (bis)phosphonate group-containing molecules, sodium Alendronate (Aln) and 3-AminoropylPhosphoric Acid (ApA), to Cellulose nanocrystals (CNCs) was performed via oxidation/Shiff-base reaction. Further fluorescent labelling with Rhodamine B Iso ThioCyanate (RBITC) was performed to follow CNCs interaction and potential internalization with/in human osteoblasts by confocal microscopy. Complementary analyses were applied to identify the conjugation (Atenuated Total Reflectance-Fourier Transform Infrared and UV-VIS spectroscopies), physico-chemical (Dynamic Light Scattering and Nanoparticle Tracking Analysis) and morphological (Transmission Electron Microscopy) features of native and ApA/Aln-modified CNCs in physiologically relevant environments (Phosphate Buffer Saline, Advanced Dulbecco's Modified Eagle Medium). While conjugation did not affect the CNCs' size, the RBITC-labelling promotes their aggregation. Faster (1 h vs. 2 h) uptake by osteoblasts of RBITCCNCoxAln, compared to RBITC-CNCoxApA, and no-internalization (in 24 h) of native RBITTC-CNC, indicate a higher affinity of Aln-modified CNCs to the cells, while all CNCs (in 0.25-0.06 wt%) promote the cell growth. Aln/Apa-modified CNCs shows high potential in drug-delivery for bone therapies, and theranostics.

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“…1,2 In particular, polymer based coatings can serve as drug delivery platforms, releasing biomolecules (antibiotics, growth factors, proteins, peptides, genes) preloaded into the coating matrix, to convert conventional implants into biologically active components. 3,4 In comparison with conventional oral or injectable therapies usually associated with side effects, such coating systems should be able to release drug molecules locally and in a controlled manner for prolonged periods.…”

Section: Introductionmentioning

confidence: 99%

Multilayered drug delivery coatings composed of daidzein-loaded PHBV microspheres embedded in a biodegradable polymer matrix by electrophoretic deposition

Chen

Yao

et al. 2016

J. Mater. Chem. B

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The development of drug delivery coating systems for local and long-term drug release is gaining increasing interest especially to functionalize bioinert implants with osseointegration and antibacterial properties. In this study, a biodegradable drug delivery coating platform consisting of drug-loaded PHBV microspheres embedded in an alginate-PVA matrix was fabricated by a one-step electrophoretic deposition (EPD) process. Layer by layer (LbL) deposition was exploited to generate chitosan-alginate multilayers on the EPD-produced coating to enlarge the diffusional barrier around the microspheres for controlled drug release. Daidzein, selected as a model drug due to its anti-osteoporosis properties, was pre-encapsulated in PHBV microspheres. The parameters for microsphere fabrication were optimized by an orthogonal design approach. The loading efficiency of daidzein in both the microspheres and in the deposited coatings was adjusted by varying the processing parameters during microsphere fabrication and the EPD process. The degradation of the deposited multilayers was investigated in PBS for up to 14 days. The degradation rate, surface roughness and wettability, as well as adhesion strength of the coatings during degradation were evaluated by applying a range of techniques. A controlled and sustained daidzein release was detected from both free microspheres and microsphere-containing coatings. Finally cytotoxicity and stimulatory effects of daidzein or daidzein-loaded coatings, on both MC3T3-E1 and RAW264.7 cell lines, were studied to validate the potential of the developed coatings for orthopedic applications.

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Cellulose Nanocrystals—Bioactive Glass Hybrid Coating as Bone Substitutes by Electrophoretic Co-deposition: In Situ Control of Mineralization of Bioactive Glass and Enhancement of Osteoblastic Performance

Cited by 69 publications

References 49 publications

Natural and Synthetic Polymers for Bone Scaffolds Optimization

Natural and Synthetic Polymers for Bone Scaffolds Optimization

Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells

Multilayered drug delivery coatings composed of daidzein-loaded PHBV microspheres embedded in a biodegradable polymer matrix by electrophoretic deposition

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