Centimeter-scale wide-field-of-view laser-scanning photoacoustic microscopy for subcutaneous microvasculature in vivo

Liao, Tangyun; Liu, Yuan; Wu, Jyh‐Horng; Deng, Lijun; Deng, Yu; Zeng, Lvming; Ji, Xuanrong

doi:10.1364/boe.426366

Cited by 15 publications

(10 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of a double-sided illumination in depth and roving scans in the transverse plane extended the spatial FOVs to 30 mm (axial) toward phantoms and 9.5 cm × 9.5 cm (lateral), which are at least tenfold larger than that of conventional PAMs. [25][26][27] The decellularization for organ transparency permits a comprehensive assessment of the vessel systems over entire organs with PAM approaches, making it accessible for large animal and even human organs in the clinical setting. In addition, utilization of the photoacoustic gradient concentration differential angiography fully contrasts the multiscale vascular networks and extensively simplify the segmentation procedure.…”

Section: Discussionmentioning

confidence: 99%

Organ‐PAM: Photoacoustic Microscopy of Whole‐organ Multiset Vessel Systems

Zhang

Peng

Qin

et al. 2023

Laser & Photonics Reviews

View full text Add to dashboard Cite

Organs maintain their unique functions through a variety of distinctive vessel systems. However, examination of multiset vessels over the entire organ still remains challenging due to the difficulties of assigning multiple vessel systems and labor-intensive imaging reconstruction technologies. Here, an organ-level photoacoustic microscopic imaging platform, named Organ-PAM, with a micron-scale spatial resolution and ultra-large field of view (FOV), which can achieve a lateral and axial FOV of up to ≈81 cm 2 and ≈30 mm, respectively, is presented. With the assistance of whole-organ decellularization, optical clearing, and a specifically designed photoacoustic gradient concentration differential angiographic pipeline, the visualization of up to four sets of vessel systems inside diversified organs is successfully achieved with multiple scales. In addition, quantitative analyses of vessel systems in both healthy livers and kidneys as well as cancerous livers with exogenous transplanted tumors are conducted at different stages. Thus, the platform enables high-efficiency multiset vessel imaging, recognition, and quantification of different organs, providing critical insights into distinct vessel systems under varying pathological conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

Organ‐PAM: Photoacoustic Microscopy of Whole‐organ Multiset Vessel Systems

Zhang

Peng

Qin

et al. 2023

Laser & Photonics Reviews

View full text Add to dashboard Cite

show abstract

“…Recently, TUTs based on the piezoelectric active element in particular have gained significant interest from multiple groups in the photoacoustic and ultrasound community due to their versatility and cost effectiveness 170–176 . For example, Chen et al 177 used LiNbO3‐based TUT to image chicken embryo vasculature and for melanoma detection.…”

Section: Current Challenges and Future Trendsmentioning

confidence: 99%

“…Reprinted with permission 148 versatility and cost effectiveness. [170][171][172][173][174][175][176] For example, Chen et al thinned-skull window. 179,180 The TUT cranial window allowed us to use three different modalities OR-PAM, TPM, and IOSI to image awake mouse brain vasculature using a head restraint setup.…”

Section: Current Challeng E S and Future Trendsmentioning

confidence: 99%

Photoacoustic imaging for microcirculation

et al. 2022

View full text Add to dashboard Cite

The microvascular networks regulate blood flow to biological tissue and are the primary site for the exchange of hormones, gasses, nutrients, water, and waste between blood and interstitial fluid. 1,2 Microvessels, i.e., arterioles, venules, and capillaries, form the microvascular networks. 1 The walls of arterioles mainly comprise a layer of endothelium and smooth muscles to allow for the flow of blood into capillaries through vasoconstriction and vasodilation, which are regulated by tissue oxygen concentration, autonomic nervous system, and hormones. 1 Capillaries, the smallest blood vessels, receive blood from arterioles; and they have a single cell thick layer of the endothelium to facilitate the exchange of nutrients and gasses between blood and interstitial fluid. 1 Capillaries terminate into venules whose walls contain fibrous tissue, which allow them to primarily act as volume reservoirs. 1 Any loss in blood flow, changes in oxygen concentration, dysregulation in the autonomic nervous system, etc. due to various pathologies, such as diabetes, sickle cell anemia, hypertension, bodily injury, and tumor growth, cause disruptions in microcirculation and, in severe cases, lead to irreparable harm and

show abstract

“…This will not only significantly reduce the beam engineering challenges but will also lead to the development of a more compact, portable, wearable, and versatile multimodal systems. For this purpose, both conventional piezoelectric materials [40][41][42][43] and capacitive micromachined ultrasound transducers (CMUTs) [44][45][46] have been studied for developing TUTs. Ilkhechi et al reported transparent CMUT array for ultrasound imaging of a small size tissue phantom [45] and photoacoustic [46] imaging of pencil leads submerged in oil tank.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, these detectors cannot be used for ultrasound excitation/imaging required for dual-modality USPA imaging applications. While prior studies demonstrated the potential of transparent lithium niobate-(LN-) based single element TUTs for high sensitivity PA imaging [40][41][42][43], TUT-arrays are required for real-time multimodal imaging.…”

Section: Introductionmentioning

confidence: 99%

A Transparent Ultrasound Array for Real-Time Optical, Ultrasound, and Photoacoustic Imaging

et al. 2022

View full text Add to dashboard Cite

Objective and Impact Statement. Simultaneous imaging of ultrasound and optical contrasts can help map structural, functional, and molecular biomarkers inside living subjects with high spatial resolution. There is a need to develop a platform to facilitate this multimodal imaging capability to improve diagnostic sensitivity and specificity. Introduction. Currently, combining ultrasound, photoacoustic, and optical imaging modalities is challenging because conventional ultrasound transducer arrays are optically opaque. As a result, complex geometries are used to coalign both optical and ultrasound waves in the same field of view. Methods. One elegant solution is to make the ultrasound transducer transparent to light. Here, we demonstrate a novel transparent ultrasound transducer (TUT) linear array fabricated using a transparent lithium niobate piezoelectric material for real-time multimodal imaging. Results. The TUT-array consists of 64 elements and centered at ~6 MHz frequency. We demonstrate a quad-mode ultrasound, Doppler ultrasound, photoacoustic, and fluorescence imaging in real-time using the TUT-array directly coupled to the tissue mimicking phantoms. Conclusion. The TUT-array successfully showed a multimodal imaging capability and has potential applications in diagnosing cancer, neurological, and vascular diseases, including image-guided endoscopy and wearable imaging.

show abstract

Centimeter-scale wide-field-of-view laser-scanning photoacoustic microscopy for subcutaneous microvasculature in vivo

Cited by 15 publications

References 57 publications

Organ‐PAM: Photoacoustic Microscopy of Whole‐organ Multiset Vessel Systems

Organ‐PAM: Photoacoustic Microscopy of Whole‐organ Multiset Vessel Systems

Photoacoustic imaging for microcirculation

A Transparent Ultrasound Array for Real-Time Optical, Ultrasound, and Photoacoustic Imaging

Contact Info

Product

Resources

About