Central migration of neuronal tissue and embryonic stem cells following transplantation along the adult auditory nerve

Hu, Zhengqing; Ulfendahl, Mats; Olivius, Petri

doi:10.1016/j.brainres.2004.08.013

Cited by 81 publications

(61 citation statements)

References 26 publications

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“…Long-term survival is necessary, of course, for auditory nerve replacement to have therapeutic value. Hu et al (2004) found moderate survival of mouse stem cell derived neurons several weeks following placement into guinea pig modiolus, but also found that the survival time of stem cells transduced into neural phenotype by expression of Ngn2 was decreased compared to the non-transduced stem cells (Hu et al., 2005). These neural stem cells demonstrated good survival at 2 weeks; but this was greatly diminished at 4 weeks post-placement (Hu et al, 2005).…”

Section: Challenge Two -Survivalmentioning

confidence: 92%

“…Hu et al (2005) tested this approach in the cochlea, transfecting Ngn2 into neural stem cells prior to placement and found the number reaching a neuronal phenotype (based on immunostaining for TUJ1) increased from 15% to over 50%. Hu et al (2004) co-transplantated mouse ESCs and a neuronal co-graft of dorsal root ganglion tissue into guinea pig cochlea, to influence stem cell differentiation in vivo. They found improved survival of TUJ1 immunolabeled stem cells at 4 weeks following placement with the co-graft.…”

Section: Differentiation Into An Appropriate Phenotypementioning

confidence: 99%

“…Hu et al (2004) have demonstrated migration of mouse embryonic dorsal root ganglion cells from the site of placement in the rat scala tympani into the modiolus with projections both peripherally toward the hair cells, as well as centrally, and formation of axonal connections between the projections of the implants and SGN central projections. Hu et al (2005) also observed mouse embryonic stem cells placed into rat scala tympani migrating into the auditory nerve and entering into the CN.…”

Section: Challenge Three -Integration and Functional Central Connectionmentioning

confidence: 99%

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Stem cell transplantation for auditory nerve replacement

2008

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The successful function of cochlear prostheses depends on activation of auditory nerve. The survival of auditory nerve neurons, however, can vary widely in candidates for cochlear implants and influence implant efficacy. Stem cells offer the potential for improving the function of cochlear prostheses and increasing the candidate pool by replacing lost auditory nerve. The first phase of studies for stem cell replacement of auditory nerve has examined the in vitro survival and differentiation as well as in vivo differentiation and survival of exogenous embryonic and tissue stem cells placed into scala tympani and/or modiolus. These studies are reviewed and new results on in vivo placement of B-5 mouse embryonic stem cells into scala tympani of the guinea pig cochleae with differentiation into a glutamatergic neuronal phenotype are presented. Research on the integration and connections of stem cell derived neurons in the cochlea is described. Finally, an alternative approach is considered, based on the use of endogenous progenitors rather than exogenous stem cells, with a review of promising findings that have identified stem cell-like progenitors in cochlear and vestibular tissues to provide the potential for auditory nerve replacement.

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Section: Challenge Two -Survivalmentioning

confidence: 92%

Section: Differentiation Into An Appropriate Phenotypementioning

confidence: 99%

Section: Challenge Three -Integration and Functional Central Connectionmentioning

confidence: 99%

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Stem cell transplantation for auditory nerve replacement

2008

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“…We review previous reports and discuss obstacles to overcome for successful functional recovery. Several kinds of pluripotent stem cells have been delivered into the cochlea for the regeneration of SGNs, including NSCs [10,13,46], ESCs [4,5,11,12,29,34,36,38], bone marrow stem cells (BMSCs) [26,28,40], and iPSCs [27]. Tamura et al, evaluated the ability of NSCs to achieve neural differentiation in the modiolus of the cochlea and demonstrated that some grafted NSCs expressed β-III tubulin, a neuronal marker, although the majority of them differentiated into glial cells [46].…”

Section: Regeneration Of Spiral Ganglion Neuronsmentioning

confidence: 99%

Potential of Pluripotent Stem Cells for the Replacement of Inner Ears

Nishimura¹,

Nakagawa²,

Ito³

2011

Embryonic Stem Cells - Recent Advances in Pluripotent Stem Cell-Based Regenerative Medicine

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“…33,37 This migratory capacity may be useful for delivery of gene therapy to infiltrative tumors like malignant gliomas.…”

Section: Embryonic Stem Cell Differentiation To Astrocytesmentioning

confidence: 99%

Gene delivery by embryonic stem cells for malignant glioma therapy: hype or hope?

Germano

Uzzaman²,

Keller³

2008

Cancer Biology & Therapy

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Patients with glioblastomas have a poor prognosis and new treatments paradigms are needed. The futility of current treatments lies in part on the difficulty of delivering therapeutic agents to the tumor. In this article, we review and discuss current knowledge of adjuvant treatment for glioblastomas including gene therapy and viral vectors. Additionally, this review provides an update on recent progress, which has focused on improving delivery methods including the development of new approaches such as stem cells. Particular emphasis is given to laboratory data showing the use of embryonic stem cells used as vectors to deliver pro-apoptotic genes to glioblastoma cells.

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Central migration of neuronal tissue and embryonic stem cells following transplantation along the adult auditory nerve

Cited by 81 publications

References 26 publications

Stem cell transplantation for auditory nerve replacement

Stem cell transplantation for auditory nerve replacement

Potential of Pluripotent Stem Cells for the Replacement of Inner Ears

Gene delivery by embryonic stem cells for malignant glioma therapy: hype or hope?

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