Chalky versus foliated: a discriminant immunogold labelling of shell microstructures in the edible oyster Crassostrea gigas

Mouchi, Vincent; Guichard, Nathalie; Immel, Françoise; Rafélis, Marc de; Broussard, Cédric; Crowley, Quentin; Marin, Frédéric

doi:10.1007/s00227-016-3040-6

Cited by 20 publications

(22 citation statements)

References 48 publications

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“…4c,d), the elements in this angular relationship can be interpreted (in a way similar to the foliated material, above) as belonging to different surfaces of the same skeletal rhombohedron. Accordingly, the reported differences in the kind of biomacromolecules between both the foliated and chalk materials 21 are not reflected in the crystallographic structure.The angular deflections of laths of the foliated microstructure at the transition to the chalk with invariant orientations (Fig. 4b) are clear cases of laths running along different surfaces of the same rhombohedron, which would in this way be of the skeletal (hollow) type.…”

Section: Discussionmentioning

confidence: 86%

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Structure and crystallography of foliated and chalk shell microstructures of the oyster Magallana: the same materials grown under different conditions

Checa

Harper

González-Segura

2018

Sci Rep

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Oyster shells are mainly composed of layers of foliated microstructure and lenses of chalk, a highly porous, apparently poorly organized and mechanically weak material. We performed a structural and crystallographic study of both materials, paying attention to the transitions between them. The morphology and crystallography of the laths comprising both microstructures are similar. The main differences were, in general, crystallographic orientation and texture. Whereas the foliated microstructure has a moderate sheet texture, with a defined 001 maximum, the chalk has a much weaker sheet texture, with a defined 011 maximum. This is striking because of the much more disorganized aspect of the chalk. We hypothesize that part of the unanticipated order is inherited from the foliated microstructure by means of, possibly, twinning. Growth line distribution suggests that during chalk formation, the mantle separates from the previous shell several times faster than for the foliated material. A shortage of structural material causes the chalk to become highly porous and allows crystals to reorient at a high angle to the mantle surface, with which they continue to keep contact. In conclusion, both materials are structurally similar and the differences in orientation and aspect simply result from differences in growth conditions.

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Section: Discussionmentioning

confidence: 86%

“…Mouchi et al . 21 have demonstrated that the biomolecular composition of the two microstructures is at least partially different, and Langlet et al . 22 have identified the appearance of growth lines within the chalk but not their relationship with those in the rest of the shell.…”

Section: Introductionmentioning

confidence: 99%

Structure and crystallography of foliated and chalk shell microstructures of the oyster Magallana: the same materials grown under different conditions

Checa

Harper

González-Segura

2018

Sci Rep

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“…10,11 Generally, oyster shells are comprised mainly of calcitic folia with some calcitic chalk. 3, [12][13][14][15] Folia can be considered as the calcite analogue of nacre with overall uniform crystallography across the Bivalvia (Figure 1). 5,[12][13][14] It has been well established that the mineral composition of oyster shell contributes to its mechanical strength.…”

Section: ■ Introductionmentioning

confidence: 99%

Crystallographic Interdigitation in Oyster Shell Folia Enhances Material Strength

Yuan¹,

Fitzer²,

Chung

et al. 2018

Crystal Growth & Design

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Shells of oyster species belonging to the genus Crassostrea have similar shell micro-structural features comprising well-ordered calcite folia. However, the mechanical strengths of folia differ dramatically between closely-related species. For example, the calcareous shells of the Hong Kong oyster Crassostrea hongkongensis, are stronger than those of its closest relative, the Portuguese oyster, Crassostrea angulata. Specifically, after removal of organic content, the folia of C. hongkongensis are 200% tougher and able to withstand a 100% higher crushing force than that of C. angulata. Detailed analyses of shell structural and mechanical features support the hypothesis that crystallographic interdigitations confer elevated mechanical strength in C. hongkongensis oyster shells compared to C. angulata shells. Consequently, the folia of C. hongkongensis is structurally equipped to withstand higher external load compared to C. angulata. The observed relationships between oyster shell structure, crystallography and mechanical properties provided an insightful context in which to consider the likely fate of these two species in future climate change scenarios. Furthermore, the interdisciplinary approach developed in this study through integrating electron backscatter diffraction (EBSD) data into finite element analysis (FEA) could be applied to other biomineral systems to investigate the relationship between crystallography and mechanical behavior.

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“…Data availability. The data used in this paper have been deposited in the Zenodo data repository (https://doi.org/10.5281/zenodo.3529419, Mouchi et al, 2019; https://doi.org/10.5281/zenodo.3731254, Mouchi et al, 2020).…”

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confidence: 99%

Rare earth elements in oyster shells: provenance discrimination and potential vital effects

et al. 2020

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Abstract. Rare earth elements (REEs) and yttrium in seawater originate from atmospheric fallout, continental weathering, and transport from rivers, as well as hydrothermal activity. Previous studies have reported the use of REE and Y measurements in biogenic carbonates as a means to reconstruct these surface processes in ancient times. As coastal seawater REE and Y concentrations partially reflect those of nearby rivers, it may be possible to obtain a regional fingerprint of these concentrations from bivalve shells for seafood traceability and environmental monitoring studies. Here, we present a dataset of 297 measurements of REE and Y abundances by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) from two species (Crassostrea gigas and Ostrea edulis). We measured a total of 49 oyster specimens from six locations in France (Atlantic Ocean and Mediterranean Sea). Our study reports that there is no significant difference in concentrations from shell parts corresponding to winter and summer periods for both species. Moreover, interspecific vital effects are reported from specimens from both species and from the same locality. REE and Y profiles as well as t-distributed stochastic neighbour embedding processing (t-SNE; a discriminant statistical method) indicate that REE and Y measurements from C. gigas shells can be discriminated from one locality to another, but this is not the case for O. edulis, which presents very similar concentrations in all studied localities. Therefore, provenance studies using bivalve shells based on REEs and Y have to first be tested for the species. Other methods have to be investigated to be able to find the provenance of some species, such as O. edulis.

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Chalky versus foliated: a discriminant immunogold labelling of shell microstructures in the edible oyster Crassostrea gigas

Abstract: contents are different. However, the direct visual evidence that some macromolecular 37

Cited by 20 publications

References 48 publications

Structure and crystallography of foliated and chalk shell microstructures of the oyster Magallana: the same materials grown under different conditions

Structure and crystallography of foliated and chalk shell microstructures of the oyster Magallana: the same materials grown under different conditions

Crystallographic Interdigitation in Oyster Shell Folia Enhances Material Strength

Rare earth elements in oyster shells: provenance discrimination and potential vital effects

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