Change of uterine histroph proteins during follicular and luteal phase in pigs

Yang

et al. 2017

Animal Science Journal

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Endometrial remodeling is important for successful embryo development and implantation in pigs. Therefore, this study investigated change of proteins regulating endometrial remodeling on follicular and luteal phase in porcine endometrial tissues. The endometrial tissue samples were collected from porcine uterus during follicular and luteal phase, vascular endothelial growth factor (VEGF), myoglobin and cysteine-rich protein 2 (CRP2) proteins were expressed by immnofluorescence, immunoblotting, and determined by 2-DE and MALDI-TOF/MS. We found that VEGF, myoglobin and CRP2 were strongly localized in endometrial tissues during luteal phase, but not follicular phase. The protein levels of VEGF, myoglobin and CRP2 in endometrial tissues were higher than luteal phase (P < 0.05). These results may provide understanding of intrauterine environment during estrous cycle in pigs, and will be used in animal reproduction for developing specific biomarkers in the future.

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The expression of VEGF, myoglobin and CRP2 proteins regulating endometrial remodeling in the porcine endometrial tissues during follicular and luteal phase

Yang

et al. 2017

Animal Science Journal

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“…Proteins into rehydration buffer were incubated with an 18 cm immobilized pH 3–11 nonlinear gradient dry strip (GE Healthcare) for 16 hours at 20°C. As described previously (19), isoelectric focusing (IEF) was performed for protein separation. IEF was performed at hold 500 V for 1 hour, gradient 1,000V 1hour, gradient 8,000 V for 3 hours, hold 8,000 V for 1.5 hours, gradient 10,000 V for 3 hours and hold 10,000 V for 1hour.…”

Section: Methodsmentioning

confidence: 99%

The Ras family members follow the blood progesterone level during formation and regression in bovine corpus luteum

2019

Preprint

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40Ras family members regulate cellular differentiation, proliferation and survival. CL formation and regression are 41 regulated by the blood P4 level. This study investigated the association between changes in Ras family members and 42 the serum P4 level and determined protein interactions among Ras family members, hormone receptors, and 43 angiogenetic and apoptotic factors during formation and regression of the bovine CL. RASAL3 and RASA3 were found 44 using proteomics in CL and were significantly increased in the SPCL compared to the PPCL, whereas RasGEF1B was 45 decreased in the PPCL. Hormone receptors and angiogenetic proteins expression was lower in the PPCL and SPCL than 46 that in the RPCL, but apoptotic proteins were increased in the RPCL. The P4 and estrogen receptors positive correlated 47 48 Ras protein expression was increased in the PPCL compared to that in the SPCL, whereas RasGEF expression was 49 decreased. In summary, Ras activation and angiogenesis in the CL were positively correlated with the blood P4 during 50 estrous cylce. These results may increase understanding of Ras biological functions following stimulation of hormones 51 and their receptors during tissue proliferation and degeneration. 53The corpus luteum (CL) is a transient endocrine gland in the female reproductive tract that produces progesterone 78 (P4), which is required to maintain pregnancy for the beginning of life in mammals (1). During CL formation, granulosa 79 and theca cells in the ovary are differentiated and proliferate into steroidogenic luteal cells (LSCs) in response to 80 luteinizing hormone (LH) until day 8 to 9 after ovulation. Then, the CL weight increases three to four times when its 81 growth is complete prior to the next round of ovulation (2). LSCs produce P4 that is secreted into the blood vessels, 82 which contribute to the maintenance of pregnancy (3-5). However, if the pregnancy is not established, the CL begins to 83 regress in response to prostaglandin F2 alpha (PGF2α) derived from the endometrium in a process termed luteolysis (6). 84The formation and regression of the CL are repeated, and this repetition by sex hormones distinguishes it from other 85 mammalian tissues (6). For several decades, studies have mainly investigated the formation and regression of 86 gonadotropin release hormone (GnRH) and steroidogenic hormones, whereas few studies have investigated the roles of 87 small GTPases in formation and regression of the CL. 88During the proliferation phase (PP), luteal endothelial cells (LECs) proliferate following interaction with vascular 89 endothelial growth factor A (VEGFA) and VEGF receptor 2 (VEGFR2) to generate blood vessels and support LSC 90 proliferation in the CL (7, 8). After vasculature is stabilized by the balance of angiopoietin 1 (Ang1) and Tie2 (9). When 91 the CL is completely developed, P4 is converted from pregneolone by 3β-hydroxysteroid dehydrogenase (3β-HSD) 92 continuously during a phase that is called the secretion phase (SP) (6). If implantation is successful, matern...

“…In the intrauterine proteome study of porcine, there were 280 protein expression spots detected using the 2D-PAGE method in porcine uterine secretion during the estrous cycle (Kayser, Kim, Cerny, & Vallet, 2006). Furthermore, the expression of the porcine histotroph proteins undergoes an increase during the luteal phase compared to the follicular phase in which there were 136 protein spots detected in the follicular stage and 217 protein spots identified in the luteal stage (Lee, Song, Hwangbo, Lee, & Park, 2016). In addition, the proteins of the cattle uterine secretion were enriched during the luteal phase in which there were 300 proteins identified on day 7 and 510 proteins detected on day 13 after the estrus stage of the estrous cycle (Mullen et al, 2012).…”

Section: Change Of Histotroph Protein In Estrous Cyclementioning

confidence: 99%

“…vascular, and extracellular matrix remodeling of the uterine morphology during the different phase of the estrous cycle (Lee, Song, Hwangbo, Lee, & Park, 2016). To that, we are inspired to discuss further related to the likelihood of a novel endometrial-secreted factors' function in the process of endometrial remodeling during the estrous cycle.…”

mentioning

confidence: 99%

The potential function of endometrial‐secreted factors for endometrium remodeling during the estrous cycle

Billhaq

2020

Animal Science Journal

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The uterine endometrium is an intricate tissue, which is comprised of luminal epithelial cells, superficial glandular epithelial cells, deep glandular epithelial cells, and fibroblast-like stromal cells (Forde & Lonergan, 2012). Uterine endometrial glands of all mammalian species synthesize and secrete a complex substance into the uterine lumen that has a function in the onset of pregnancy, the growth of the placenta, and conceptus development, termed histotroph (Gray et al., 2001). This uterine glandular secretion, histotroph, performs a vital role in providing a source of nutrient for the embryo and involving in fetoplacental development during the embryo implantation (Burton, Watson, Hempstock, Skepper, & Jauniaux, 2002). In the global proteomic characterization study, the histotroph compounds are proteins, carbohydrates, sugars, lipids, and ions (Kayser, Kim, Cerny, & Vallet, 2006; Beltman et al., 2014). Thus far, uterine luminal fluids are well known as prominent substrates, and nutrients that are required to support the establishment of pregnancy confirmed in the previous study of ovine uterine gland knockout model (Gray et al., 2000; Forde & Lonergan, 2012). In formerly research of porcine uterine fluids, we discovered that the endometrial-secreted factors contained essential proteins associated with the color, thickness,