Changed betaxanthin pattern in violet flowers of Portulaca grandiflora after the feeding of DOPA

Rink, Erika; Böhm, H.

doi:10.1016/s0031-9422(00)81046-8

Cited by 13 publications

(4 citation statements)

References 16 publications

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“…In order to verify the identity of the betaxanthins synthesized we hydrolyzed a sample of the respective reaction product by acid as described earlier (Rink & B6hm 1985). After its isolation, the amino acid released was compared with authentic substance (Merck or Reanal) in three chromatographic systems.…”

Section: Partial Synthesis Of Betaxanthinsmentioning

confidence: 99%

“…One of the advantages of this species (Konar 1978;Schr6der & B6hm 1984;Rink & B6hm 1985;Riederer & Zryd 1986) is that it shows various flower colours, the genetics of which has extensively been studied (Ikeno 1921;Adachi et al 1985). One of the advantages of this species (Konar 1978;Schr6der & B6hm 1984;Rink & B6hm 1985;Riederer & Zryd 1986) is that it shows various flower colours, the genetics of which has extensively been studied (Ikeno 1921;Adachi et al 1985).…”

Section: Introductionmentioning

confidence: 99%

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Establishment and characterization of a betaxanthin-producing cell culture from Portulaca grandiflora

Böhm

Rink

1991

Plant Cell Tiss Organ Cult

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Cell cultures derived from three yellow flowering Portulaca grandiflora genotypes contained betacyanins rather than betaxanthins. A betaxanthin-producing cell culture was obtained by subculturing orange cell clusters isolated from the red-violet cell culture of a violet flowering P. grandiflora genotype. Selection of the most strongly yellow coloured cell material reduced the portion of betacyanins considerably and resulted in a P. grandiflora cell culture characterized by a high concentration of betaxanthins and the occurrence of free betalamic acid. Vulgaxanthin I was the main compound. Besides these pigments carotenoids and flavonoids were detectable.Betaxanthin biosynthesis strongly dependend on light. Product accumulation reached its maximum during the stationary phase of the growth cycle. Excretion of pigments, especially of betalains, could not be detected. Vulgaxanthin I as found in the cell culture was identical with one of two main betaxanthins in the yellow petals of P. grandiflora.The yellow P. grandiflora cells grew well on solid modified Murashige and Skoog medium but failed to grow in liquid medium after a few subcultures. In contrast, white P. grandiflora suspension cultures could be established repeatedly.

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Section: Partial Synthesis Of Betaxanthinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of a betaxanthin-producing cell culture from Portulaca grandiflora

Böhm

Rink

1991

Plant Cell Tiss Organ Cult

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“…rubrum (Berlin et al 1986). Studies on other systems, however, demonstrated the stimulation of betalain biosynthesis through administration of DOPA (French et al 1974;Elliott 1983;Rink and B6hm 1985).…”

Section: Discussionmentioning

confidence: 99%

Accumulation of phenolic acid conjugates and betacyanins, and changes in the activities of enzymes involved in feruloylglucose metabolism in cell-suspension cultures of Chenopodium rubrum L.

Bokern

Wray

Strack

1991

Planta

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Cell-suspension cultures of Chenopodium rubrum accumulate various soluble secondary phenolic metabolites such as the hydroxybenzoic acid glycosides 4-hydroxybenzoic acid-β-glucoside, vanillic acid-β-glucoside, the hydroxycinnamic acid acylglycosides 1-O-(4-coumaroyl)-β-glucose, 1-O-feruloyl-β-glucose, 1-O-sinapoyl-β-glucose and 1-O-feruloyl-(β-1,2-glucuronosyl)-β-glucose, the hydroxycinnamic acid amide N-feruloylaspartate, and the betacyanins betanin, amaranthin and celosianin II. In addition, accumulation of the insoluble cell wall-bound hydroxycinnamic acids with ferulic acid as the major component occurs parallel to culture growth. The changes of three pivotal enzymatic activities, all O-transferases which are involved in the formation of the dominant ferulic acid conjugates, were determined. These are (i) uridine 5'-diphosphate(UDP)glucose-hydroxycinnamic acid O-glucosyltransferase (EC 2.4.1), (ii) UDP-glucuronic acid:1-O-hydroxycin-namoyl-β-glucose O-glucuronosyltransferase (EC 2.4.1) and (iii) 1-O-hydroxycinnamoyl-β-glucose:amaranthin O-hydroxycinnamoyltransferase (EC 2.3.1). The patterns of metabolite accumulation associated with these enzyme activities show that the hydroxycinnamic acid-glucose esters play a central role as metabolically active intermediates in the secondary metabolism of Ch. rubrum. Two cell lines of this culture (CH, CHN), differing in their betacyanin content, were compared with respect to this metabolism. A markedly higher total betacyanin content in the CHN line might possibly be the consequence of an increased supply of the key precursor for betalain biosynthesis, i.e. 3,4-dihydroxyphenylalanine (DOPA). In addition, the enhanced accumulation of celosianin II in the CHN line correlates well with a higher activity of the enzyme catalyzing the transfer of ferulic acid from 1-O-feruloyl-β-glucose to amaranthin.

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“…Natural colors are materials extracted, isolated, or otherwise derived from plants, animals, or minerals that are capable of imparting a color when added to the formulations. Important classes of natural colors are carotenoids, tetrapyrrole, phycocyanin, anthocyanin, and indolic biochromes [4]. Natural colors provide more a more natural look to products with respect to the glossy and brightness of synthetic colors [5].…”

Section: Introductionmentioning

confidence: 99%

Production of Betalains From Hairy Root Culture of Beta Vulgaris and Its Use in Paracetamol Syrup as a Natural Colourant

Madhavan

2019

Asian J Pharm Clin Res

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Objective: The present study was aimed to extract betalains from hairy root culture of Beta vulgaris and its use in pharmaceutical formulations as a colorant. Methods: Hairy roots were initiated using different strains of Agrobacterium rhizogenes such as A.2/83, A.20/83, A.4, and LMG 150; LMG 150 was found to initiate a large number of hairy roots, and betalain content was estimated. Paracetamol syrup was prepared using extracted betalains as a colorant at different concentrations of 10 and 30 mg/150 ml. Stability studies were carried out at a different temperatures such as 25°C, 30°C, and 40°C) and light (dark, 1000 and 2000 lux) for 45 days. Results: In case of a concentration of 10 mg/150 ml syrup, the effects of temperature such as 25°C, 30°C, and 40°C and dark condition on the degradation of betalains were found to be 48%, 88%, and 100% in 45 days, respectively. The effects of temperature such as 25°C, 30°C, and 40°C at light 1000 lux on degradations of betalains were found to be 81% and 98% at 25°C and 30°C in 45 days, respectively, and 100% at 40°C in 30 days and at 2000 lux were found to be 100% at 25°C and 30°C in 30 days and 100% at 40°C in 10 days. The similar levels of degradation rate were observed with a concentration of 30 mg/150 ml. Conclusion: Experimental data demonstrated that formulation with betalains exhibited better stability at the dark condition and lower temperature.

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Changed betaxanthin pattern in violet flowers of Portulaca grandiflora after the feeding of DOPA

Cited by 13 publications

References 16 publications

Establishment and characterization of a betaxanthin-producing cell culture from Portulaca grandiflora

Establishment and characterization of a betaxanthin-producing cell culture from Portulaca grandiflora

Accumulation of phenolic acid conjugates and betacyanins, and changes in the activities of enzymes involved in feruloylglucose metabolism in cell-suspension cultures of Chenopodium rubrum L.

Production of Betalains From Hairy Root Culture of Beta Vulgaris and Its Use in Paracetamol Syrup as a Natural Colourant

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