Changes in Histones/DNA Ratio in Scutellum Nuclei During Ageing of<i>Triticum Durum</i>Caryopses

Innocenti, A. M.; Bitonti, Maria Beatrice

doi:10.1080/00087114.1981.10796882

Cited by 4 publications

(1 citation statement)

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“…Due to the low percentage of nuclei with a 16C DNA content, we were unable to separate this class of nuclei during the flow cytometric analysis of the isolated organs of Brachypodium embryos. Endoreplicated nuclei with an 8C and 16C DNA content were identified in the parenchyma cells of the scutellum of Triticum durum [ 34 ]. The scutellum and aleurone layers play an essential role in the germination process by producing hydrolytic enzymes in order to mobilise the storage compounds of the starchy endosperm, which support early seedling development [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Germination and the Early Stages of Seedling Development in Brachypodium distachyon

Wolny

Betekhtin

Rojek

et al. 2018

IJMS

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Successful germination and seedling development are crucial steps in the growth of a new plant. In this study, we investigated the course of the cell cycle during germination in relation to grain hydration in the model grass Brachypodium distachyon (Brachypodium) for the first time. Flow cytometry was performed to monitor the cell cycle progression during germination and to estimate DNA content in embryo tissues. The analyses of whole zygotic embryos revealed that the relative DNA content was 2C, 4C, 8C, and 16C. Endoreplicated nuclei were detected in the scutellum and coleorhiza cells, whereas the rest of the embryo tissues only had nuclei with a 2C and 4C DNA content. This study was accompanied by a spatiotemporal profile analysis of the DNA synthetic activity in the organs of Brachypodium embryos during germination using EdU labelling. Upon imbibition, nuclear DNA replication was initiated in the radicle within 11 h and subsequently spread towards the plumule. The first EdU-labelled prophases were observed after 14 h of imbibition. Analysis of selected genes that are involved in the regulation of the cell cycle, such as those encoding cyclin-dependent kinases and cyclins, demonstrated an increase in their expression profiles.

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Section: Discussionmentioning

confidence: 99%