Changes in milk fat globule membrane proteome after pasteurization in human, bovine and caprine species

Ma, Ying; Zhang, Lina; Wu, Yanyan; Zhou, Peng

doi:10.1016/j.foodchem.2018.12.015

Cited by 78 publications

(56 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protein profile is vast, and it varies between species. Human, bovine, and caprine MFGM possess 1104, 632, and 137 different proteins, respectively [35]. However, a group of eight proteins are usually the most abundant in all species.…”

Section: Compositionmentioning

confidence: 99%

“…In addition to the predominant group shown in Table 3, hundreds of other proteins in minor concentrations are also found, and according to proteomic studies, we know that the distribution varies greatly upon the stage of lactation, environmental conditions, the method of extraction, and the processing of the source containing MFGM. These minor proteins have important functions in biological processes, such as antioxidant and detoxification properties, and molecular functions such as binding [35]. Finally, it is important to note that, according to the techniques used to isolate MFGM and process milk, whey proteins (αlactalbumin, β-lactoglobulin, immunoglobulins, and lactoferrin) are often found associated with the MFGM fraction [34].…”

Section: Compositionmentioning

confidence: 99%

See 1 more Smart Citation

Improving Human Health with Milk Fat Globule Membrane, Lactic Acid Bacteria, and Bifidobacteria

et al. 2021

View full text Add to dashboard Cite

The milk fat globule membrane (MFGM), the component that surrounds fat globules in milk, and its constituents have gained significant attention for their gut function, immune-boosting properties, and cognitive-development roles. The MFGM can directly interact with probiotic bacteria, such as bifidobacteria and lactic acid bacteria (LAB), through interactions with bacterial surface proteins. With these interactions in mind, increasing evidence supports a synergistic effect between MFGM and probiotics to benefit human health at all ages. This important synergy affects the survival and adhesion of probiotic bacteria through gastrointestinal transit, mucosal immunity, and neurocognitive behavior in developing infants. In this review, we highlight the current understanding of the co-supplementation of MFGM and probiotics with a specific emphasis on their interactions and colocalization in dairy foods, supporting in vivo and clinical evidence, and current and future potential applications.

show abstract

Section: Compositionmentioning

confidence: 99%

Section: Compositionmentioning

confidence: 99%

Improving Human Health with Milk Fat Globule Membrane, Lactic Acid Bacteria, and Bifidobacteria

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Milk fat globule membrane (MFGM) secreted from the apical membrane carries a part of the cytoplasmic fraction of MECs, and they also represent partial information on the proteome of terminally differentiated MECs 23 . Studies on MFGM proteome reported a total of 632, 137, 947 and 1104 proteins in cattle, goat, donkey, and human respectively 24,25 . On the other hand, a proteomics study on mice mammary tissue suggested the biological significance of RNA polymerase B transcription factor 3 (BTF3) in the differentiation of MECs during pregnancy 26 .…”

Section: Discussionmentioning

confidence: 99%

In-depth proteome analysis of more than 12,500 proteins in buffalo mammary epithelial cell line identifies protein signatures for active proliferation and lactation

Jaswal

Anand

Kumar

et al. 2020

Sci Rep

View full text Add to dashboard Cite

The mature mammary gland is made up of a network of ducts that terminates in alveoli. The innermost layer of alveoli is surrounded by the differentiated mammary epithelial cells (MECs), which are responsible for milk synthesis and secretion during lactation. However, the MECs are in a state of active proliferation during pregnancy, when they give rise to network like structures in the mammary gland. Buffalo (Bubalus bubalis) constitute a major source of milk for human consumption, and the MECs are the major precursor cells which are mainly responsible for their lactation potential. The proteome of MECs defines their functional state and suggests their role in various cellular activities such as proliferation and lactation. To date, the proteome profile of MECs from buffalo origin is not available. In the present study, we have profiled in-depth proteome of in vitro cultured buffalo MECs (BuMECs) during active proliferation using high throughput tandem mass spectrometry (MS). MS analysis identified a total of 8330, 5970, 5289, 4818 proteins in four sub-cellular fractions (SCFs) that included cytosolic (SCF-I), membranous and membranous organelle's (SCF-II), nuclear (SCF-III), and cytoskeletal (SCF-IV). However, 792 proteins were identified in the conditioned media, which represented the secretome. Altogether, combined analysis of all the five fractions (SCFs-I to IV, and secretome) revealed a total of 12,609 non-redundant proteins. The KEGG analysis suggested that these proteins were associated with 325 molecular pathways. Some of the highly enriched molecular pathways observed were metabolic, MAPK, PI3-AKT, insulin, estrogen, and cGMP-PKG signalling pathway. The newly identified proteins in this study are reported to be involved in NOTCH signalling, transport and secretion processes.The mammary gland, a unique organ of mammals, is a derivative of ventral skin 1 and has evolved to provide nutrition and immune protection to the offspring. Though the anatomical and physiological features of a mammary gland are diverse, the basic structure is similar in all species. The mature mammary gland is constituted by a network of tubular-alveolar structures, with their lumen lined by a layer of polarized secretory mammary epithelial cells (MECs). The MECs are dynamic components of the mammary gland, which undergo repeated rounds of proliferation and differentiation during different developmental stages such as pregnancy, and lactation 2 . The quantity and functional differentiation of MECs in the udder determine the milk-producing ability in livestock. Bovine (ruminants) are physiologically distinct from other mammals such as humans, mice and rats. The present study on in-depth proteome analysis of buffalo (Bubalus bubalis) MECs (BuMECs) advances our knowledge on the protein machinery operating in mammary gland development and lactogenesis in buffalo, which can be extrapolated in other mammalian species as well. Among the domestic livestock species, cattle (Bos taurus) and

show abstract

“…K-casein, a protein present in human milk, has a C-terminal whose sequence is similar to that of mucin [24]. The concentration of this protein is increased after Holder pasteurization of human milk and could potentially prevent the attachment of bacteria to the mucus glycoproteins [25]. Mannose-binding lectins (MBL) are proteins also detected in human milk whose binding activity is not affected by Holder pasteurization [26,27].…”

Section: Role Of Dhm On the Adhesion Of Probioticsmentioning

confidence: 99%

“…Depending on the glycosylation and degradation degrees of the intestinal mucus, this could indicate that human milk MBLs with high affinity for MUC2 mannose receptors could potentially bind to the isolated mucus and interfere with the probiotic adhesion. In addition to that, mucin 1, which is thermally stable after pasteurization and rich in mannose structures, has been identified as one of the major mucins of human milk [25,29,30]. A recent analysis of the LGG genome has revealed two lectin-like proteins, Llp1 and Llp2, with high binding specificity for D-mannose and mannan [31].…”

Section: Role Of Dhm On the Adhesion Of Probioticsmentioning

confidence: 99%

The Effect of Donor Human Milk Fortification on The Adhesion of Probiotics In Vitro

et al. 2020

View full text Add to dashboard Cite

Preterm delivery complications are the primary cause of death among children under the age of five. Preventive strategies include the use of pasteurized donor human milk (DHM), its fortification with human milk fortifiers (protein supplements), and supplementation with probiotics. Our aim was to examine the impact of DHM and fortified DHM (FDHM) on the mucus adhesion properties of two widely used probiotics. The study covered two forms of human milk fortifier, liquid and powdered, with or without probiotics and storage at 4 °C for 24 h. To test the adhesion properties of the probiotic strains, DHM+probiotics and FDHM+probiotics were prepared and added to immobilized mucus isolated from the stool of healthy Finnish infants. The probiotic adhesion was then measured by liquid scintillation. Our results suggest that addition of liquid or powdered human milk fortifier in donor human milk had no impact on probiotic adhesion. In addition, given the increased adhesion of probiotics suspended in buffer, other matrices should be further studied. These factors need to be considered when designing future intervention strategies using probiotics in preterm infants.

show abstract

Changes in milk fat globule membrane proteome after pasteurization in human, bovine and caprine species

Cited by 78 publications

References 31 publications

Improving Human Health with Milk Fat Globule Membrane, Lactic Acid Bacteria, and Bifidobacteria

Improving Human Health with Milk Fat Globule Membrane, Lactic Acid Bacteria, and Bifidobacteria

In-depth proteome analysis of more than 12,500 proteins in buffalo mammary epithelial cell line identifies protein signatures for active proliferation and lactation

The Effect of Donor Human Milk Fortification on The Adhesion of Probiotics In Vitro

Contact Info

Product

Resources

About