Changes in Peripheral Lymphocyte Subsets in the Type 1 Diabetic Dogs Treated with Insulin Injections

Mori, Akira; Sagara, Fumi; Shimizu, Sumire; Mizutani, H.; Sako, Toshinori; Hirose, Hisashi; Yoshimura, Itaru; Uematsu, Yosuke; Yamaguchi, Takahiro; Arai, Toshiro

doi:10.1292/jvms.70.185

Cited by 10 publications

(11 citation statements)

References 18 publications

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“…Interestingly, the plasma IRI levels also significantly increased (approximately doubling). Explanation for the increase in IRI is that the IRI analysis method used in our study may cross-react with the insulin preparation used for administration [35]. As such, higher IRI values were observed after undergoing intensive glycemic control treatment.…”

Section: Discussionmentioning

confidence: 88%

“…The plasma adiponectin concentration was measured with a commercial kit (Mouse/Rat Adiponectin ELISA Kit, Otsuka, Tokyo, Japan). Plasma immunoreactive insulin (IRI) concentrations were measured by the ELISA method described previously [35].…”

Section: Animalsmentioning

confidence: 99%

“…Although, intensive insulin treatment should be considered and probably recommended for most patients with type 1 DM [13], its implementation is difficult due to the rigorous requirement of constant monitoring of the patient to prevent hypoglycemia. It has been reported that diabetic dogs with inadequate glycemic control have immune system abnormalities [35], especially increased susceptibility to infections [26] and a higher risk for rapid development of cataracts [17]. Interestingly, a prominent feature during immune response is an increase of glucose utilization by white blood cells.…”

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confidence: 99%

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Successful Intensive Insulin Treatment of Type 1 Diabetic Dogs Leads to Restoration of Peripheral Leukocyte Insulin Signaling Gene Expression and Enzyme Activities

Mori

Lee

Sako

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. The purpose of this study was to investigate whether intensive insulin treatment of dogs suffering from type 1 diabetes mellitus, resulting in tight glycemic control, could be reflected by changes in peripheral leukocyte metabolism. Specifically, plasma metabolites and enzyme activities were assessed. In addition, quantitative RT-PCR was used to determine changes in insulin signaling gene (insulin receptor substrate (IRS)-1, IRS-2 and phosphatidylinositol 3-kinase (PI3-K) P85) mRNA levels in peripheral leukocytes. Lastly, leukocyte enzymes involved in cellular energy metabolism were examined for changes in glucose utilization. Our results indicated that intensive insulin treatment was successful in type 1 DM dogs, leading to tight glycemic control. The mean glucose concentration and glycated albumin percentage significantly decreased to 156 mg/dl and 15.6%, respectively, following treatment. In peripheral leukocytes, the IRS-2 and PI3-K p85 mRNA levels significantly increased, and a significant increase in pyruvate kinase and pyruvate carboxylase activity, two enzymes involved in cellular energy metabolism, was also observed post treatment. Therefore, the observed changes in insulin signaling pathway activity and cellular energy metabolism enzyme activity in peripheral leukocytes are considered to be characteristics of amelioration of glucose metabolism by insulin action. As such, peripheral leukocytes are sufficiently sensitive to monitor for improving glycemic control during intensive insulin treatment of type 1 DM dogs. Blood cells such as leukocytes are much more readily available than muscle or adipose tissue for studies in dogs.KEY WORDS: diabetes mellitus, dogs, insulin receptor substrate, intensive glycemic control, leukocytes.

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Section: Discussionmentioning

confidence: 88%

Section: Animalsmentioning

confidence: 99%

mentioning

confidence: 99%

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Successful Intensive Insulin Treatment of Type 1 Diabetic Dogs Leads to Restoration of Peripheral Leukocyte Insulin Signaling Gene Expression and Enzyme Activities

Mori

Lee

Sako

et al. 2009

The Journal of Veterinary Medical Science

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“…Our rats led to an insulin deWcient state by STZ administration presented an opposite behavior than that observed in insulin-treated type 1 diabetes patients. This might be a result of the insulin deWcient state, as this hormone is a crucial growth factor and function limiting specially for lymphocytes (Mori et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Neutrophil response of anaerobic jump trained diabetic rats

et al. 2008

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This paper investigated the effect of jump training on blood biochemical parameters and neutrophil responses of diabetic rats. Male Wistar rats were divided into control, trained, diabetic and trained-diabetic groups. Diabetes was induced by i.v. injection of streptozotocin. Jump training consisted of six sets of ten jumps in water with overload of 50% of body mass with 1-min of resting, four times per week during 6 weeks. Plasma glucose, lactate, triacylglycerol and total cholesterol concentrations, differential leukocyte count, phagocytosis and anion superoxide production by neutrophils were evaluated. Diabetes caused hyperglycemia, hypertriacylglycerolemia, and body weight loss. Physical training reversed hypertriacylglycerolemia. Jump training increased phagocytosis and anion superoxide production by blood neutrophils from trained and trained-diabetic rats. Neutrophilia and lymphocytopenia occur in diabetic and trained-diabetic rats. Anaerobic jump training in diabetic rats reduced hypertriacylglycerolemia and increased neutrophil anion superoxide production. Phagocytosis was not altered in trained-diabetic rats.

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“…Absolute values for lymphocyte subsets were calculated using counts obtained from WBC analysis in combination with the flow cytometer. These procedures were conducted in accordance with Mori et al (2008).…”

Section: Flow Cytometric Analysis Of Peripheral Bloodmentioning

confidence: 99%

Investigations into the lymphocyte phenotypes and the presence of rheumatoid factor and antinuclear antibody in the peripheral blood of 515 dogs

Tamura¹,

Nagashima²,

Oda³

et al. 2012

Vet. Med. - Czech

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ABSTRACT:The levels of rheumatoid factor (RF), antinuclear antibody (ANA), and composition of peripheral lymphocyte subsets in 515 dogs were examined. Of these sample cases, 33 cases were diagnosed as immune-mediated fever that presented with high C-reactive protein (CRP), 31 cases were diagnosed with Hansen's Type 1 disc herniation and the remaining 415 cases were clinically healthy dogs, and served as controls. In the cases diagnosed with immune-mediated fever, 84% of the dogs tested positive to either RF or ANA (RF positive 60.6%; ANA positive 24.2%). By contrast, 16.2% of the healthy dogs were positive for either RF or ANA (RF positive 14.9%; ANA 1.3%). The CD4/CD8 ratio for peripheral lymphocyte was high for all analysed cases diagnosed with immune-mediated fever, and was significantly higher than those of healthy controls. These results indicate that the abnormal levels of lymphocytes may be an effective indicator for immune-mediated disease coupled to immune-mediated fever.

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Changes in Peripheral Lymphocyte Subsets in the Type 1 Diabetic Dogs Treated with Insulin Injections

Cited by 10 publications

References 18 publications

Successful Intensive Insulin Treatment of Type 1 Diabetic Dogs Leads to Restoration of Peripheral Leukocyte Insulin Signaling Gene Expression and Enzyme Activities

Successful Intensive Insulin Treatment of Type 1 Diabetic Dogs Leads to Restoration of Peripheral Leukocyte Insulin Signaling Gene Expression and Enzyme Activities

Neutrophil response of anaerobic jump trained diabetic rats

Investigations into the lymphocyte phenotypes and the presence of rheumatoid factor and antinuclear antibody in the peripheral blood of 515 dogs

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