Changes in Phospholipid/Ceramide Profiles and Eicosanoid Levels in the Plasma of Rats Irradiated with UV Rays and Treated Topically with Cannabidiol

Abstract

Chronic UV radiation causes oxidative stress and inflammation of skin and blood cells. Therefore, in this study, we assessed the effects of cannabidiol (CBD), a natural phytocannabinoid with antioxidant and anti-inflammatory properties, on the phospholipid (PL) and ceramide (CER) profiles in the plasma of nude rats irradiated with UVA/UVB and treated topically with CBD. The results obtained showed that UVA/UVB radiation increased the levels of phosphatidylcholines, lysophospholipids, and eicosanoids (PGE2, TxB2), while downregulation of sphingomyelins led to an increase in CER[NS] and CER[NDS]. Topical application of CBD to the skin of control rats significantly upregulated plasma ether-linked phosphatidylethanolamines (PEo) and ceramides. However, CBD administered to rats irradiated with UVA/UVB promoted further upregulation of CER and PEo and led to significant downregulation of lysophospholipids. This was accompanied by the anti-inflammatory effect of CBD, manifested by a reduction in the levels of proinflammatory PGE2 and TxB2 and a dramatic increase in the level of anti-inflammatory LPXA4. It can therefore be suggested that topical application of CBD to the skin of rats exposed to UVA/UVB radiation prevents changes in plasma phospholipid profile resulting in a reduction of inflammation by reducing the level of LPE and LPC species and increasing antioxidant capacity due to upregulation of PEo species.

Characterization of the Antitumor Potential of Extracts of Cannabis sativa Strains with High CBD Content in Human Neuroblastoma

“…In this context, we found that long-chain PCs that contained polyunsaturated fatty acids (PUFAs) were increased in the plant extract-treated cells, particularly PC 38:5 and PC 38:6. These data agree with the elevation of these long-chain PCs that was reported after situations of oxidative stress in [ 39 ]. By contrast, in our study, PCs with three unsaturations or less generally decreased due to the action of the Cannabis plant extracts.…”

Evidence of Cannabidiol Effectiveness Associated or Not with Tetrahydrocannabinol in Topical Administration: A Scope Review

“…Furthermore, a study presented a possible multidirectional anti-inflammatory effect of CBD after topical application in UVA/B exposure skins. Plasma samples demonstrated downregulation of relevant phospholipids (lyso-PE and lyso-PC), prostaglandin, and thromboxane, although, it also demonstrated upregulation of anti-inflammatory lipoxin (LPXA 4 ) [ 50 ].…”

Role of ceramides in diabetic foot ulcers (Review)

“…Deficiency of this type of ceramide may be associated with seasonal skin dryness ( 39 ). Exposure to ultraviolet irradiation can increase plasma ceramide levels and decrease SM levels in mice ( 40 ). Therefore, the level of epidermal ceramides is maintained in dynamic equilibrium by control from a number of factors and plays an important role in skin homeostasis ( 41 ).…”