Changes in reactive oxygen species begin early during replicative aging of Saccharomyces cerevisiae cells

Lam, Yuen Ting; Aung-Htut, May T.; Lim, Yu L.; Yang, Hongyuan; Dawes, Ian W.

doi:10.1016/j.freeradbiomed.2011.01.013

Cited by 55 publications

(65 citation statements)

References 47 publications

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“…Mitochondrial fragmentation begins to occur in cells after four generations and increases as cells age, and mitochondria are severely fragmented with no tubular structure at the 11th generation (39). These observations are consistent with our observations of aging cells at the 11th generation.…”

Section: Discussionsupporting

confidence: 92%

Changes in Transcription and Metabolism During the Early Stage of Replicative Cellular Senescence in Budding Yeast

Kamei

Tamada

Nakayama

et al. 2014

Journal of Biological Chemistry

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Background: Senescence is the biological process of age-related cellular and organismal deterioration in function. Results: The transcriptome and metabolome at early stages of replicative senescence of yeast cells were measured. Conclusion: The transcriptomic and metabolomic profiles drastically changed at about half of the average replicative lifespan. Significance: This is the first integrated information on transcriptome and metabolome of aging yeast cells.

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Section: Discussionsupporting

confidence: 92%

Changes in Transcription and Metabolism During the Early Stage of Replicative Cellular Senescence in Budding Yeast

Kamei

Tamada

Nakayama

et al. 2014

Journal of Biological Chemistry

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“…Serial growth and sorting allows progressively older populations of mother cells to be obtained and analyzed. This procedure has been used successfully to examine changes in cell physiology and gene expression during replicative aging of yeast 13,14,17,18 . The current method adapts this biotin labeling and magnetic sorting technique to enrich for mother cells with the purpose of measuring the accumulation of potentially rare mutations or other forms of genome instability assayed through phenotypic selections.…”

Section: Introductionmentioning

confidence: 99%

Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in <em>Saccharomyces cerevisiae</em>

Patterson

Maxwell

2014

JoVE

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Saccharomyces cerevisiae has been an excellent model system for examining mechanisms and consequences of genome instability. Information gained from this yeast model is relevant to many organisms, including humans, since DNA repair and DNA damage response factors are well conserved across diverse species. However, S. cerevisiae has not yet been used to fully address whether the rate of accumulating mutations changes with increasing replicative (mitotic) age due to technical constraints. For instance, measurements of yeast replicative lifespan through micromanipulation involve very small populations of cells, which prohibit detection of rare mutations. Genetic methods to enrich for mother cells in populations by inducing death of daughter cells have been developed, but population sizes are still limited by the frequency with which random mutations that compromise the selection systems occur. The current protocol takes advantage of magnetic sorting of surface-labeled yeast mother cells to obtain large enough populations of aging mother cells to quantify rare mutations through phenotypic selections. Mutation rates, measured through fluctuation tests, and mutation frequencies are first established for young cells and used to predict the frequency of mutations in mother cells of various replicative ages. Mutation frequencies are then determined for sorted mother cells, and the age of the mother cells is determined using flow cytometry by staining with a fluorescent reagent that detects bud scars formed on their cell surfaces during cell division. Comparison of predicted mutation frequencies based on the number of cell divisions to the frequencies experimentally observed for mother cells of a given replicative age can then identify whether there are age-related changes in the rate of accumulating mutations. Variations of this basic protocol provide the means to investigate the influence of alterations in specific gene functions or specific environmental conditions on mutation accumulation to address mechanisms underlying genome instability during replicative aging.

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“…63 Strikingly, when SIR2 is deleted, daughter cells have lower levels of ROS compared to WT daughters despite having more fragmented mitochondria and being shorter lived. 53 Additionally, in some cases, ROS levels in wild type yeast increase to high levels even in young cells. 53 Consequently, it has been proposed that ROS and mitochondrial dysfunctions, which increase with age, lead to the activation of a stress response mechanism called mitochondrial hormesis that promotes longevity.…”

Section: Segregating Fitness: Mother Cells Giving the Best To Their Bmentioning

confidence: 99%

A budding yeast's perspective on aging: The shape I'm in

Smith

Wright

Schneider

2015

Exp Biol Med (Maywood)

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Aging is exemplified by progressive, deleterious changes that increase the probability of death. However, while the effects of age are easy to recognize, identification of the processes involved has proved to be much more difficult. Somewhat surprisingly, research using the budding yeast has had a profound impact on our current understanding of the mechanisms involved in aging. Herein, we examine the biological significance and implications surrounding the observation that genetic pathways involved in the modulation of aging and the determination of lifespan in yeast are highly complicated and conserved.

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Changes in reactive oxygen species begin early during replicative aging of Saccharomyces cerevisiae cells

Cited by 55 publications

References 47 publications

Changes in Transcription and Metabolism During the Early Stage of Replicative Cellular Senescence in Budding Yeast

Changes in Transcription and Metabolism During the Early Stage of Replicative Cellular Senescence in Budding Yeast

Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in <em>Saccharomyces cerevisiae</em>

A budding yeast's perspective on aging: The shape I'm in

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