Changes in Serum Protein Profile, Cholesterol and Blood Glucose during Endotoxic Shock in Buffalo Calves Supplemented with Vitamin E and Selenium

Abstract

A study was conducted to monitor the changes in serum protein profile, cholesterol and blood glucose during endotoxic shock in buffalo calves and also to assess the role of prophylactic supplementation of vitamin E and selenium in alleviating the endotoxic effects. Fifteen male buffalo calves (6-8 months of age) were divided into three groups: Group I (control)-infused with 0.9% saline solution; Group II-infused with E. coli endotoxin at 5 µg/kg body weight in normal saline solution; Group III-supplemented prophylactically with 250 mg vitamin E and 7.5 mg selenium by i/m injections at weekly intervals for one month prior to the induction of endotoxic shock. The blood samples were collected at 0, 1, 3, 6, 9, 12, 24, 48 and 72 h after the induction of shock. Endotoxin caused a significant (p<0.05) hypoproteinemia from 3-12 h post infusion in group II but this hypoproteinemia was less pronounced and only from 3-9 h post infusion in vitamin E and selenium supplemented calves. Hypoglycemia was observed in group II from 3-24 h and blood glucose level returned to normal at 72 h. However hypoglycemia was mild in group III and blood glucose returned to normal at 48 h. Hypocholesterolaemia and hypoalbuminemia were found in both groups II and III but these changes were less pronounced in group III i.e. vitamin E and Se supplemented calves. Serum electrophoretic protein patterns of group III were quite similar to those of control group but animals of group II had different electrophoretic pattern. It was concluded that the antioxidant effects of vitamin E and Se prevent the liver against oxidative stress during endotoxic shock.

Cholesterol and Triglycerides Concentrations of Lipopolysaccharide-Induced Inflammatory Male Rat in Response to Petiveria alliacea L. Leaf Extract

“…The LPS directly cause dysfunction of lipid metabolism, thereby causing hepatotoxicity, renal failure and lipid peroxidation via the induction of free radicals 20,22,23 . This study agrees with these metabolic disorders and organ failure caused by LPS.…”

Effect of Rumen-protected Choline Addition on Milk Performance and Blood Metabolic Parameters in Transition Dairy Cows

“…Meanwhile, Pinotti et al (2004) observed that choline as a component of lipoprotein could increase plasma folate and vitamin E status in periparturient dairy cows. It has been observed that vitamin E has a positive effect on the immune system, and vitamin E supplementation may reduce oxidative stress in periparturient cows (Chatterjee et al, 2003) and buffalo heifers (Kahlon et al, 2004;Sharma et al, 2005).…”

Perinatal Nutrition of the Calf and Its Consequences for Lifelong Productivity

“…The immune status of the buffalo calf post-colostrum feeding is influenced also by the vitamin status of the dam: circulating Ig levels were increased by 80% in calves fed colostrum from their dams receiving bolus injections of vitamins A., D 3 and E late in pregnancy (Sikka and Lal, 2005). VitE and selenium also limit the adverse effects of endotoxin from E coli infections associated with many calf rearing systems (Sharma et al, 2005).…”